LOCUS       BBa_K1214011             976 bp    DNA     linear       10-Dec-2025
DEFINITION  pFru-RBS-GFP
ACCESSION   BBa_K1214011
VERSION     BBa_K1214011.1  
FEATURES             Location/Qualifiers
     promoter        1..180
                     /label=BBa_K1214007
     RBS             189..203
                     /label=RBS-1\Strong
     RBS             196..200
                     /label=RBS
     misc_feature    189..203
                     /label=BBa_B0030
     RBS             189..203
                     /label=BBa_B0030
     CDS             210..929
                     /label=GFP protein
     CDS             210..929
                     /label=BBa_E0040
     misc_feature    938..976
                     /label=B1006
     stem_loop       947..968
                     /label=modified thr terminator
     polyA_site      969..976
                     /label=PolyA
     polyA_site      938..946
                     /label=PolyA
     terminator      938..976
                     /label=BBa_B1006
     misc_feature    189..976
                     /label=BBa_K081012
ORIGIN
        1 aaaattgtgc agcacatcaa acttttgctc ataactttac ggctttcctt gcgtgctgaa
       61 acagaagtat tatgctttct tgaaacgttt cagcgcgatc ttgtctttaa ccctaagcca
      121 ggttggcgct ttttttctca tagaggctga atcgtttcaa ttcagcaaga gaggagaact
      181 tactagagat taaagaggag aaatactaga tgcgtaaagg agaagaactt ttcactggag
      241 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      301 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      361 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
      421 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
      481 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
      541 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
      601 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
      661 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
      721 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      781 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
      841 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
      901 atggcatgga tgaactatac aaataataat actagagaaa aaaaaacccc gcccctgaca
      961 gggcggggtt tttttt
//