LOCUS BBa_K1216004 1890 bp DNA linear 26-Nov-2024 DEFINITION β-Glucuronidase (gusA) from Bacillis Subtilis with TEV and poly-HIS tags ACCESSION BBa_K1216004 VERSION BBa_K1216004.1 FEATURES Location/Qualifiers CDS 31..1839 /label=gusA misc_feature 1840..1860 /label=TEV-tag misc_feature 1867..1884 /label=HIS-tag ORIGIN 1 tttgtttaac tttaagaagg agatatacat atgttacgtc ctgtagaaac cccaacccgt 61 gaaatcaaaa aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt 121 gatcagcgtt ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt 181 tttaacgatc agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag 241 cgcgaagtct ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg 301 gtcactcatt acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc 361 tatacgccat ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc 421 accgtttgtg tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc 481 gacgaaaacg gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc 541 catcgcagcg taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg 601 acgcatgtcg cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt 661 gatgtcagcg ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact 721 agcgggactt tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat 781 gaactgtgcg tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc 841 atccggtcag tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt 901 actggctttg gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg 961 atggtgcacg accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat 1021 tacccttacg ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat 1081 gaaactgctg ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag 1141 ccgaaagaac tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag 1201 gcgattaaag agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt 1261 gccaacgaac cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa 1321 gcaacgcgta aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac 1381 gctcacaccg ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga 1441 tggtatgtcc aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg 1501 gcctggcagg agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta 1561 gccgggctgc actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg 1621 gatatgtatc accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat 1681 ttcgccgatt ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc 1741 ttcactcgcg accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc 1801 atgaacttcg gtgaaaaacc gcagcaggga ggcaaacaag agaatcttta ttttcagggc 1861 ctcgagcacc accaccacca ccactaataa //