LOCUS BBa_K1216005 1536 bp DNA linear 29-Nov-2024 DEFINITION Alkaline phosphatase (phoA) from Citrobacter with TEV and poly-HIS tags ACCESSION BBa_K1216005 VERSION BBa_K1216005.1 FEATURES Location/Qualifiers misc_feature 1486..1506 /label=TEV-tag CDS 31..1485 /label=phoA misc_feature 1513..1520 /label=HIS-tag ORIGIN 1 tttgtttaac tttaagaagg agatatacat atgtttttta ttttaactgt atttatacat 61 ggagaaaata aagtgaaaca aagcgctatg atttttgcct tgctgccttt gctgttggct 121 ccggccagtc aggcggacac cacaacaata tccgtgctgg ataatcgtgc ggcgcagggc 181 gatattacta cgcccggcgg cgcgcgccga ttaaccggcg atcaaacggc ggcgctgcgt 241 gaatcgctaa atgataaacc ggcgaaaaat attattctgc tgatcggcga cggaatggga 301 gattctgaaa taaccgccgc acgaaattat gccgaaggcg ccggcggctt ttttaaaggt 361 attgatgccc tgccgctgac cggacaatat acccactatg cgctgaataa aaaaaccggc 421 aaaccagatt acgtgaccga ttccgccgcc tcagccaccg cgtggtccac aggggtgaaa 481 acctacaacg gcgcgctggg ggtcgatatt catgaaaaag atcatctgac cattctcgaa 541 atggcgaagg ccgcaggtct ggctaccggc aacgtctcga ccgcggagtt gcaggacgcc 601 accccggcag cgctgattgc ccatgtcacc tcgcgcaaat gctatggccc gacggcgacc 661 agcgaaaaat gtccgacaaa cgcgctggaa aagggcggca aaggctcgat tactgaacag 721 ctcctgaacg cgcgggcgga cgtcaccctg ggcggcggcg cgaaaacctt cgccgagacg 781 gcaaccgccg gcgagtggca ggggaaaacg ctgcgtgagc aggcgcagat gcgcggctac 841 cagttagtcg gcgatgccgc ctctttagcg gcgatcgacg aagcgaatca ggacaaaccg 901 ctgctgggac tgttcgcaga ggggaatatg ccggtgcgct gggaagggcc gaaggcctcg 961 taccacggca atattgataa acctgccgtg acctgtacgc caaatccgaa acgcaatgac 1021 gcgattccca cgctggcgca gatgaccgac aaagccatcg aactgttgag caaaaatgag 1081 cggggcttct ttttacaggt ggaaggcgcg tctatcgata agcaggatca cgccgcgaac 1141 ccgtgcggac agattggcga gacggtggat cttgatgaag ccgtacagag ggcgctggcc 1201 tttgcgaaga aagacggcaa tacgctggtg atcgttaccg ccgatcatgc tcatgccagc 1261 cagatcgtgg cgcccgaaac gaaagcgccg gggctgacgc aggcgctgaa caccaaagat 1321 ggcgcggtga tggtcatcag ctacggcaac tcggaagaag attcccagga gcataccggc 1381 agccagctgc gcatcgccgc ttacgggccg catgcggcga acgtggtcgg gctgaccgat 1441 caaaccgatc tgttctacac catgaaagcg gcgctgggcc tgaaggagaa tctttatttt 1501 cagggcctcg agcaccacca ccaccaccac taataa //