LOCUS       BBa_K1217000            2078 bp    DNA     linear       21-May-2024
DEFINITION  pT7-ppk1(T.forsythia)
ACCESSION   BBa_K1217000
VERSION     BBa_K1217000.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..2078
                     /label=ppk1
     CDS             39..2078
                     /label=BBa_K1217001
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat ggaagagaca ggatatcgct
       61 attttaaacg tgacgtcagt tggctgtcgt ttaattatcg ggtattactc gaggcctcag
      121 acgaaacgct tccaatctat gaacggattc gtttcctgtc catctatgcg tcgaatttag
      181 aagagtttta cgaaatccgg gtggccgaac atcgtggtac gattatgaag gggattttta
      241 cggccggaga cgtaggactg gcggaagaaa cgctgagcga gattacggat gaggtcaacc
      301 gtcagcagca agccttctac cggattttca acgaaagaat cctgcctgag ttacgtcggc
      361 aaaagatctg cctctatcag agtgaagagc cggagccgtt tcatcgcgag tttgtccgca
      421 attattttaa cgaggaaatt tttccgtatc tctctcccgt aacgattcat gccggtttcc
      481 gcacttttat tcgtgaccgg cgtctatatc tgatcacccg catcatccgg aaagagacgg
      541 gggaatcgct ttacgtactt attaaagtgc cgcattccaa ggtgtcgcgt tttattgcct
      601 tgcctccgca taagggcatg cactatttca tgtttatcga cgatgtgatc cgttacaacc
      661 tgcccagcat ttttcccggc tatgaattgg acggatgcta cagcatcaaa atttcgcgcg
      721 atgccgatat ttacgtcgaa gaggagacgt cgcagactat tgtacaggcc atacgccgta
      781 aggtggggaa gcgcaagatc ggagctttga gtcgttttat gtacgaccgt gccatgccgc
      841 gcgattttct ggcgtatatt tgcgaggctt tcgatatcgc tgatgatgat cttgttgtcg
      901 gtggccgtta tatgaatctt caggatatga gcaaactgcc gaatcctgtc ggtcaggggc
      961 tcgaacagca gattccgtcg cccatgcgcg tgccgtatct cgaagaggcg gcgtcgatgt
     1021 ttggcgccgt aaaagcacgt gacgtattgc tgtatttccc ttatcagtcg ttcgactatc
     1081 tgcttcgttt cctgatggaa gcggcgttcg atccggatgt ggaggagatc aagatcacgc
     1141 agtaccgcgt agccgaaaac tcggcggtga ttcattcgct gatcagtgcc gcgcaaaacg
     1201 gaaagaaggt gacggtgttc gtcgagctga aagcgcgttt cgatgaagag aacaacatga
     1261 gtacggccga aaagatgaaa aaagccggca tcaaaatcat ttacagtatt ccgggactca
     1321 aggttcatgc gaaggtggct gtcattctgc gtaaaggaga agcgacggat tttgcctatg
     1381 tgagtaccgg caattttaac gagaaaacgg cgaaaatcta cgccgacatg gctttgctga
     1441 catgcaacaa ggcgattgtc gacgatatca accgggtctt ttccgtgctg gaagggacgc
     1501 ttcaggagcc gcaatttagt tgtttgcttg tcgcgcgttt caacatggtg ccggaactga
     1561 ttcgtatgat tcgtcgcgag acggaacatg tgaaagccgg caggaagggt cgaattgttt
     1621 tgaaaatgaa tggtctgcac gaccggagaa tgatcgacga gctgtatcgt gccggttcgg
     1681 cgggtgtaga gatcgacctg ttggtgcgcg gtatctgctg tctggtgccc gaccagccgt
     1741 atagtccgaa tatccgtgtg acgcggattg tagacatgtt tctcgaacat gcgcgtatct
     1801 ggtattttta taacgatggg gcggaagatc tgtatcttac ctcgtcggac tggatgaacc
     1861 gtaacctgaa tcgtcgcatc gagacggctg ctcccgtgct gtcgccatcc gtcaaacgtg
     1921 aaatgatcga catcctgcat atccagttgc aggataacgt caaggcttgc catatcgatg
     1981 cgcagttacg gaatgtcttc gttcgtaacg acgcgccgcc catacgtgcc cagcaggcta
     2041 tttacgacta catcaaagca aagaccgagg gctgttag
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