BBa_K1217000 1 BBa_K1217000 pT7-ppk1(T.forsythia) 2013-09-14T11:00:00Z 2015-05-08T01:09:42Z The coding gene ppk1(E.C. 2.7.4.1) is amplified from Tannerella forsythia (ATCC 43037) genomic DNA. Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria. false false _1531_ 0 13531 9 It's complicated false We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites. false Ma Tsz Shan component2342588 1 BBa_K525998 component2342590 1 BBa_K1217001 annotation2342588 1 BBa_K525998 range2342588 1 1 32 annotation2342590 1 BBa_K1217001 range2342590 1 39 2078 BBa_K1217001 1 BBa_K1217001 ppk1(T.forsythia) 2013-09-14T11:00:00Z 2015-05-08T01:09:42Z It is from Tannerella forsythia (ATCC 43037) genomic DNA. Coding sequence of polyphosphate kinase (ppk1)(E.C. 2.7.4.1) from Tannerella forsythia (ATCC 43037), ppk1 catalyzes the formation of polyphosphate from ATP. false false _1531_ 0 13531 9 Not in stock false We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites. false Ma Tsz Shan annotation2342577 1 ppk1 range2342577 1 1 2040 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2127788 1 B0034 range2127788 1 21 32 annotation2129433 1 RBS range2129433 1 24 27 BBa_K1217001_sequence 1 atggaagagacaggatatcgctattttaaacgtgacgtcagttggctgtcgtttaattatcgggtattactcgaggcctcagacgaaacgcttccaatctatgaacggattcgtttcctgtccatctatgcgtcgaatttagaagagttttacgaaatccgggtggccgaacatcgtggtacgattatgaaggggatttttacggccggagacgtaggactggcggaagaaacgctgagcgagattacggatgaggtcaaccgtcagcagcaagccttctaccggattttcaacgaaagaatcctgcctgagttacgtcggcaaaagatctgcctctatcagagtgaagagccggagccgtttcatcgcgagtttgtccgcaattattttaacgaggaaatttttccgtatctctctcccgtaacgattcatgccggtttccgcacttttattcgtgaccggcgtctatatctgatcacccgcatcatccggaaagagacgggggaatcgctttacgtacttattaaagtgccgcattccaaggtgtcgcgttttattgccttgcctccgcataagggcatgcactatttcatgtttatcgacgatgtgatccgttacaacctgcccagcatttttcccggctatgaattggacggatgctacagcatcaaaatttcgcgcgatgccgatatttacgtcgaagaggagacgtcgcagactattgtacaggccatacgccgtaaggtggggaagcgcaagatcggagctttgagtcgttttatgtacgaccgtgccatgccgcgcgattttctggcgtatatttgcgaggctttcgatatcgctgatgatgatcttgttgtcggtggccgttatatgaatcttcaggatatgagcaaactgccgaatcctgtcggtcaggggctcgaacagcagattccgtcgcccatgcgcgtgccgtatctcgaagaggcggcgtcgatgtttggcgccgtaaaagcacgtgacgtattgctgtatttcccttatcagtcgttcgactatctgcttcgtttcctgatggaagcggcgttcgatccggatgtggaggagatcaagatcacgcagtaccgcgtagccgaaaactcggcggtgattcattcgctgatcagtgccgcgcaaaacggaaagaaggtgacggtgttcgtcgagctgaaagcgcgtttcgatgaagagaacaacatgagtacggccgaaaagatgaaaaaagccggcatcaaaatcatttacagtattccgggactcaaggttcatgcgaaggtggctgtcattctgcgtaaaggagaagcgacggattttgcctatgtgagtaccggcaattttaacgagaaaacggcgaaaatctacgccgacatggctttgctgacatgcaacaaggcgattgtcgacgatatcaaccgggtcttttccgtgctggaagggacgcttcaggagccgcaatttagttgtttgcttgtcgcgcgtttcaacatggtgccggaactgattcgtatgattcgtcgcgagacggaacatgtgaaagccggcaggaagggtcgaattgttttgaaaatgaatggtctgcacgaccggagaatgatcgacgagctgtatcgtgccggttcggcgggtgtagagatcgacctgttggtgcgcggtatctgctgtctggtgcccgaccagccgtatagtccgaatatccgtgtgacgcggattgtagacatgtttctcgaacatgcgcgtatctggtatttttataacgatggggcggaagatctgtatcttacctcgtcggactggatgaaccgtaacctgaatcgtcgcatcgagacggctgctcccgtgctgtcgccatccgtcaaacgtgaaatgatcgacatcctgcatatccagttgcaggataacgtcaaggcttgccatatcgatgcgcagttacggaatgtcttcgttcgtaacgacgcgccgcccatacgtgcccagcaggctatttacgactacatcaaagcaaagaccgagggctgttag BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K1217000_sequence 1 taatacgactcactatagggaaagaggagaaatactagatggaagagacaggatatcgctattttaaacgtgacgtcagttggctgtcgtttaattatcgggtattactcgaggcctcagacgaaacgcttccaatctatgaacggattcgtttcctgtccatctatgcgtcgaatttagaagagttttacgaaatccgggtggccgaacatcgtggtacgattatgaaggggatttttacggccggagacgtaggactggcggaagaaacgctgagcgagattacggatgaggtcaaccgtcagcagcaagccttctaccggattttcaacgaaagaatcctgcctgagttacgtcggcaaaagatctgcctctatcagagtgaagagccggagccgtttcatcgcgagtttgtccgcaattattttaacgaggaaatttttccgtatctctctcccgtaacgattcatgccggtttccgcacttttattcgtgaccggcgtctatatctgatcacccgcatcatccggaaagagacgggggaatcgctttacgtacttattaaagtgccgcattccaaggtgtcgcgttttattgccttgcctccgcataagggcatgcactatttcatgtttatcgacgatgtgatccgttacaacctgcccagcatttttcccggctatgaattggacggatgctacagcatcaaaatttcgcgcgatgccgatatttacgtcgaagaggagacgtcgcagactattgtacaggccatacgccgtaaggtggggaagcgcaagatcggagctttgagtcgttttatgtacgaccgtgccatgccgcgcgattttctggcgtatatttgcgaggctttcgatatcgctgatgatgatcttgttgtcggtggccgttatatgaatcttcaggatatgagcaaactgccgaatcctgtcggtcaggggctcgaacagcagattccgtcgcccatgcgcgtgccgtatctcgaagaggcggcgtcgatgtttggcgccgtaaaagcacgtgacgtattgctgtatttcccttatcagtcgttcgactatctgcttcgtttcctgatggaagcggcgttcgatccggatgtggaggagatcaagatcacgcagtaccgcgtagccgaaaactcggcggtgattcattcgctgatcagtgccgcgcaaaacggaaagaaggtgacggtgttcgtcgagctgaaagcgcgtttcgatgaagagaacaacatgagtacggccgaaaagatgaaaaaagccggcatcaaaatcatttacagtattccgggactcaaggttcatgcgaaggtggctgtcattctgcgtaaaggagaagcgacggattttgcctatgtgagtaccggcaattttaacgagaaaacggcgaaaatctacgccgacatggctttgctgacatgcaacaaggcgattgtcgacgatatcaaccgggtcttttccgtgctggaagggacgcttcaggagccgcaatttagttgtttgcttgtcgcgcgtttcaacatggtgccggaactgattcgtatgattcgtcgcgagacggaacatgtgaaagccggcaggaagggtcgaattgttttgaaaatgaatggtctgcacgaccggagaatgatcgacgagctgtatcgtgccggttcggcgggtgtagagatcgacctgttggtgcgcggtatctgctgtctggtgcccgaccagccgtatagtccgaatatccgtgtgacgcggattgtagacatgtttctcgaacatgcgcgtatctggtatttttataacgatggggcggaagatctgtatcttacctcgtcggactggatgaaccgtaacctgaatcgtcgcatcgagacggctgctcccgtgctgtcgccatccgtcaaacgtgaaatgatcgacatcctgcatatccagttgcaggataacgtcaaggcttgccatatcgatgcgcagttacggaatgtcttcgttcgtaacgacgcgccgcccatacgtgcccagcaggctatttacgactacatcaaagcaaagaccgagggctgttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z