BBa_K1217000
1
BBa_K1217000
pT7-ppk1(T.forsythia)
2013-09-14T11:00:00Z
2015-05-08T01:09:42Z
The coding gene ppk1(E.C. 2.7.4.1) is amplified from Tannerella forsythia (ATCC 43037) genomic DNA.
Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217000, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.
false
false
_1531_
0
13531
9
It's complicated
false
We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites.
false
Ma Tsz Shan
component2342588
1
BBa_K525998
component2342590
1
BBa_K1217001
annotation2342588
1
BBa_K525998
range2342588
1
1
32
annotation2342590
1
BBa_K1217001
range2342590
1
39
2078
BBa_K1217001
1
BBa_K1217001
ppk1(T.forsythia)
2013-09-14T11:00:00Z
2015-05-08T01:09:42Z
It is from Tannerella forsythia (ATCC 43037) genomic DNA.
Coding sequence of polyphosphate kinase (ppk1)(E.C. 2.7.4.1) from Tannerella forsythia (ATCC 43037), ppk1 catalyzes the formation of polyphosphate from ATP.
false
false
_1531_
0
13531
9
Not in stock
false
We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites.
false
Ma Tsz Shan
annotation2342577
1
ppk1
range2342577
1
1
2040
BBa_K525998
1
pT7
Promoter T7 and RBS
2011-09-12T11:00:00Z
2015-05-08T01:12:35Z
Synthesis
Released HQ 2013
Promoter T7 and RBS
false
false
_690_
0
8543
9
In stock
false
Synthesis
false
Anna Drong
annotation2129432
1
T7 promoter
range2129432
1
1
20
annotation2127788
1
B0034
range2127788
1
21
32
annotation2129433
1
RBS
range2129433
1
24
27
BBa_K1217001_sequence
1
atggaagagacaggatatcgctattttaaacgtgacgtcagttggctgtcgtttaattatcgggtattactcgaggcctcagacgaaacgcttccaatctatgaacggattcgtttcctgtccatctatgcgtcgaatttagaagagttttacgaaatccgggtggccgaacatcgtggtacgattatgaaggggatttttacggccggagacgtaggactggcggaagaaacgctgagcgagattacggatgaggtcaaccgtcagcagcaagccttctaccggattttcaacgaaagaatcctgcctgagttacgtcggcaaaagatctgcctctatcagagtgaagagccggagccgtttcatcgcgagtttgtccgcaattattttaacgaggaaatttttccgtatctctctcccgtaacgattcatgccggtttccgcacttttattcgtgaccggcgtctatatctgatcacccgcatcatccggaaagagacgggggaatcgctttacgtacttattaaagtgccgcattccaaggtgtcgcgttttattgccttgcctccgcataagggcatgcactatttcatgtttatcgacgatgtgatccgttacaacctgcccagcatttttcccggctatgaattggacggatgctacagcatcaaaatttcgcgcgatgccgatatttacgtcgaagaggagacgtcgcagactattgtacaggccatacgccgtaaggtggggaagcgcaagatcggagctttgagtcgttttatgtacgaccgtgccatgccgcgcgattttctggcgtatatttgcgaggctttcgatatcgctgatgatgatcttgttgtcggtggccgttatatgaatcttcaggatatgagcaaactgccgaatcctgtcggtcaggggctcgaacagcagattccgtcgcccatgcgcgtgccgtatctcgaagaggcggcgtcgatgtttggcgccgtaaaagcacgtgacgtattgctgtatttcccttatcagtcgttcgactatctgcttcgtttcctgatggaagcggcgttcgatccggatgtggaggagatcaagatcacgcagtaccgcgtagccgaaaactcggcggtgattcattcgctgatcagtgccgcgcaaaacggaaagaaggtgacggtgttcgtcgagctgaaagcgcgtttcgatgaagagaacaacatgagtacggccgaaaagatgaaaaaagccggcatcaaaatcatttacagtattccgggactcaaggttcatgcgaaggtggctgtcattctgcgtaaaggagaagcgacggattttgcctatgtgagtaccggcaattttaacgagaaaacggcgaaaatctacgccgacatggctttgctgacatgcaacaaggcgattgtcgacgatatcaaccgggtcttttccgtgctggaagggacgcttcaggagccgcaatttagttgtttgcttgtcgcgcgtttcaacatggtgccggaactgattcgtatgattcgtcgcgagacggaacatgtgaaagccggcaggaagggtcgaattgttttgaaaatgaatggtctgcacgaccggagaatgatcgacgagctgtatcgtgccggttcggcgggtgtagagatcgacctgttggtgcgcggtatctgctgtctggtgcccgaccagccgtatagtccgaatatccgtgtgacgcggattgtagacatgtttctcgaacatgcgcgtatctggtatttttataacgatggggcggaagatctgtatcttacctcgtcggactggatgaaccgtaacctgaatcgtcgcatcgagacggctgctcccgtgctgtcgccatccgtcaaacgtgaaatgatcgacatcctgcatatccagttgcaggataacgtcaaggcttgccatatcgatgcgcagttacggaatgtcttcgttcgtaacgacgcgccgcccatacgtgcccagcaggctatttacgactacatcaaagcaaagaccgagggctgttag
BBa_K525998_sequence
1
taatacgactcactatagggaaagaggagaaa
BBa_K1217000_sequence
1
taatacgactcactatagggaaagaggagaaatactagatggaagagacaggatatcgctattttaaacgtgacgtcagttggctgtcgtttaattatcgggtattactcgaggcctcagacgaaacgcttccaatctatgaacggattcgtttcctgtccatctatgcgtcgaatttagaagagttttacgaaatccgggtggccgaacatcgtggtacgattatgaaggggatttttacggccggagacgtaggactggcggaagaaacgctgagcgagattacggatgaggtcaaccgtcagcagcaagccttctaccggattttcaacgaaagaatcctgcctgagttacgtcggcaaaagatctgcctctatcagagtgaagagccggagccgtttcatcgcgagtttgtccgcaattattttaacgaggaaatttttccgtatctctctcccgtaacgattcatgccggtttccgcacttttattcgtgaccggcgtctatatctgatcacccgcatcatccggaaagagacgggggaatcgctttacgtacttattaaagtgccgcattccaaggtgtcgcgttttattgccttgcctccgcataagggcatgcactatttcatgtttatcgacgatgtgatccgttacaacctgcccagcatttttcccggctatgaattggacggatgctacagcatcaaaatttcgcgcgatgccgatatttacgtcgaagaggagacgtcgcagactattgtacaggccatacgccgtaaggtggggaagcgcaagatcggagctttgagtcgttttatgtacgaccgtgccatgccgcgcgattttctggcgtatatttgcgaggctttcgatatcgctgatgatgatcttgttgtcggtggccgttatatgaatcttcaggatatgagcaaactgccgaatcctgtcggtcaggggctcgaacagcagattccgtcgcccatgcgcgtgccgtatctcgaagaggcggcgtcgatgtttggcgccgtaaaagcacgtgacgtattgctgtatttcccttatcagtcgttcgactatctgcttcgtttcctgatggaagcggcgttcgatccggatgtggaggagatcaagatcacgcagtaccgcgtagccgaaaactcggcggtgattcattcgctgatcagtgccgcgcaaaacggaaagaaggtgacggtgttcgtcgagctgaaagcgcgtttcgatgaagagaacaacatgagtacggccgaaaagatgaaaaaagccggcatcaaaatcatttacagtattccgggactcaaggttcatgcgaaggtggctgtcattctgcgtaaaggagaagcgacggattttgcctatgtgagtaccggcaattttaacgagaaaacggcgaaaatctacgccgacatggctttgctgacatgcaacaaggcgattgtcgacgatatcaaccgggtcttttccgtgctggaagggacgcttcaggagccgcaatttagttgtttgcttgtcgcgcgtttcaacatggtgccggaactgattcgtatgattcgtcgcgagacggaacatgtgaaagccggcaggaagggtcgaattgttttgaaaatgaatggtctgcacgaccggagaatgatcgacgagctgtatcgtgccggttcggcgggtgtagagatcgacctgttggtgcgcggtatctgctgtctggtgcccgaccagccgtatagtccgaatatccgtgtgacgcggattgtagacatgtttctcgaacatgcgcgtatctggtatttttataacgatggggcggaagatctgtatcttacctcgtcggactggatgaaccgtaacctgaatcgtcgcatcgagacggctgctcccgtgctgtcgccatccgtcaaacgtgaaatgatcgacatcctgcatatccagttgcaggataacgtcaaggcttgccatatcgatgcgcagttacggaatgtcttcgttcgtaacgacgcgccgcccatacgtgcccagcaggctatttacgactacatcaaagcaaagaccgagggctgttag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z