BBa_K1217002
1
BBa_K1217002
ppk1(K.oralis)
2013-09-14T11:00:00Z
2015-05-08T01:09:42Z
It is from Kingella oralis (ATCC 51147) genomic DNA
Coding sequence of polyphosphate kinase (ppk1)(E.C. 2.7.4.1) from Kingella oralis (ATCC 51147), ppk1 catalyzes the formation of polyphosphate from ATP.
false
false
_1531_
0
13531
9
Not in stock
false
We choose to amplify ppk1 gene from Tannerella forsythia (ATCC 43037) since the sequence does not contain any of the illegal restriction sites.
false
Ma Tsz Shan
annotation2343057
1
ppk1
range2343057
1
1
2052
BBa_K1217003
1
BBa_K1217003
pT7-ppk1(K.oralis)
2013-09-14T11:00:00Z
2015-05-08T01:09:42Z
The coding gene ppk1(E.C. 2.7.4.1) is amplified from Kingella oralis (ATCC 51147) genomic DNA.
Polyphosphate kinase (ppk1) (E.C. 2.7.4.1) catalyzes the formation of polyphosphate from ATP. In BBa_K1217003, ppk1 was under the control of T7 promotor. Under iptg induction, over-expression of ppk1 can reduce the phosphate level in the medium by its incorporation into polyphosphate synthesis inside the bacteria.
false
false
_1531_
0
13531
9
In stock
true
We choose to amplify ppk1 gene from Kingella oralis (ATCC 51147) since the sequence does not contain any of the illegal restriction sites.
false
Ma Tsz Shan
component2343090
1
BBa_K525998
component2343092
1
BBa_K1217002
annotation2343090
1
BBa_K525998
range2343090
1
1
32
annotation2343092
1
BBa_K1217002
range2343092
1
39
2090
BBa_K525998
1
pT7
Promoter T7 and RBS
2011-09-12T11:00:00Z
2015-05-08T01:12:35Z
Synthesis
Released HQ 2013
Promoter T7 and RBS
false
false
_690_
0
8543
9
In stock
false
Synthesis
false
Anna Drong
annotation2129432
1
T7 promoter
range2129432
1
1
20
annotation2127788
1
B0034
range2127788
1
21
32
annotation2129433
1
RBS
range2129433
1
24
27
BBa_K1217002_sequence
1
atgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga
BBa_K525998_sequence
1
taatacgactcactatagggaaagaggagaaa
BBa_K1217003_sequence
1
taatacgactcactatagggaaagaggagaaatactagatgccgcaatctgctcacattctttgccgcgaactgtcgctgttggcgtttaaccgccgcgtgttggcgcaagcgcaagacccgcgcgtgccgctgttggaacgactgcgctttttgtgcatcgtgtcgtccaatctggacgaattttttgaagtgcgcatggcgtatttgcgccgtgaacaacgcgccaaccccacgcaagtgttccaatcgggacaaacgcccagcgaaaccatcacgctggtggcgcaagaagcgcacaagattattgaagagcaatatcaaatatttaaccaagaattgctgcccgcgcttgccgaggaaaatattgtgttttaccgccgcaagcagtggacaagcgcgcagcgcgaatggattgaagattatttcaaccgcgagctgctgccgattctcacgccgattggcttggatgcctcgcatccgtttccgcgtccgctgaataaatcgttgaattttgtggtggaattggatggcaaagatgcgtttggacgcgcttcgggcatggcaatcgtgcaagcgccgcgcattttgccgcgtgcgattcggctgcctgaaaatatttgcgaaggcaaagacggctttgtgtttttgtcgtccattgtgcatgagtatgtgtacaagctgtttcgcggcatgacggtgaaaggctgccatcaatttcgcctaacgcgcgatagcgatttgacggtggaaaacgatgatttgagcaacctgcgcacggcggtgcaaaatgaattgcgcgaccgcgattttggcgacggagtgcggctggaaattgccgaccaatgtcctgattatattagccaatttttgctggaacagtttaagctcacgcgcaatgagttgtatcaagtggaaggtcctgtgaatttggtgcggctgatgtcggtgcccgatatggtgaaccgtcccgatttgaaattcgcgccgttcacgccgcaatatccgcgcgcgttgcgcaaatcggcatcgctgttggacgaaatcgctaagggcgatattttgctgcaccatccgtatcaatcgtttgatccgattgtgaaatttatcaacgatgccacgcgcgatcctgatgtggtggcgattaagatgacgatttaccgcacgggtagccattccgagctggcgcgcgctttgatggcggcggcgctggcgggcaagcaagttaccgtggtggtggaattgatggcgcgttttgacgaagcgaataatgtgtcgtgggcaaccaagctggaagacgcgggcgcgcacgtggtttatggcgtgtttggctacaaggtgcacgccaaaatggcgttggtgattcgccgcgaacaaggcagcctgaaaacctatgcgcatctgggcacgggcaattatcaccaaggcacatcgcgcatctacaccgatttcggcttgctcaccgccaacacggcgataacgcgcgacatcaacacgctatttatggaaatcaccggcttggggcaggcgaacaagctgaaaaaaatctaccaaagcccgttcacgctgcacaaaatgattatggacagcatcgcgcgcgaaaccgaaaacgcgaaaaacggcaagcctgcgcggattatcgccaagctcaattcgctgattgagccgcaagtgatagaggcgttgtatgccgccagcgcggcgggggtgcagattgatttgattgtgcgtggcatgtgcgctttgcgcccacaagtggcggggctgtcggaaaacatccgcgtgcgctccattattgggcggctgctggaacacgcgcgggtttattatttttacaacgatggcgcggaaaacgtgtttatttccagcgcggattggatggggcgcaattttttcagccgcattgaaacctgcacgccgattgaatcgcccgcgctcaaacagcgcgtgatagaagagggcttactgctggcgttgcgcgacaacgtgaaggcttggcagatgcacggcgacggcagctacacgcggattgtggcggacggcgacgaggtgggcattcaagaaacgctgttggcgcgatatacggaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z