LOCUS       BBa_K1217004            2135 bp    DNA     linear       26-Dec-2024
DEFINITION  Signal sequence fused ppk1 (T.forsythis) under T7 promoter
ACCESSION   BBa_K1217004
VERSION     BBa_K1217004.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..95
                     /label=Eut MCP-targeting sequence
     CDS             96..2135
                     /label=BBa_K1217001
     CDS             39..2135
                     /label=BBa_K1217006
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat ggatcaaaaa cagattgaag
       61 aaattgtacg tagcgtgatg gcgtcaatgg gacagatgga agagacagga tatcgctatt
      121 ttaaacgtga cgtcagttgg ctgtcgttta attatcgggt attactcgag gcctcagacg
      181 aaacgcttcc aatctatgaa cggattcgtt tcctgtccat ctatgcgtcg aatttagaag
      241 agttttacga aatccgggtg gccgaacatc gtggtacgat tatgaagggg atttttacgg
      301 ccggagacgt aggactggcg gaagaaacgc tgagcgagat tacggatgag gtcaaccgtc
      361 agcagcaagc cttctaccgg attttcaacg aaagaatcct gcctgagtta cgtcggcaaa
      421 agatctgcct ctatcagagt gaagagccgg agccgtttca tcgcgagttt gtccgcaatt
      481 attttaacga ggaaattttt ccgtatctct ctcccgtaac gattcatgcc ggtttccgca
      541 cttttattcg tgaccggcgt ctatatctga tcacccgcat catccggaaa gagacggggg
      601 aatcgcttta cgtacttatt aaagtgccgc attccaaggt gtcgcgtttt attgccttgc
      661 ctccgcataa gggcatgcac tatttcatgt ttatcgacga tgtgatccgt tacaacctgc
      721 ccagcatttt tcccggctat gaattggacg gatgctacag catcaaaatt tcgcgcgatg
      781 ccgatattta cgtcgaagag gagacgtcgc agactattgt acaggccata cgccgtaagg
      841 tggggaagcg caagatcgga gctttgagtc gttttatgta cgaccgtgcc atgccgcgcg
      901 attttctggc gtatatttgc gaggctttcg atatcgctga tgatgatctt gttgtcggtg
      961 gccgttatat gaatcttcag gatatgagca aactgccgaa tcctgtcggt caggggctcg
     1021 aacagcagat tccgtcgccc atgcgcgtgc cgtatctcga agaggcggcg tcgatgtttg
     1081 gcgccgtaaa agcacgtgac gtattgctgt atttccctta tcagtcgttc gactatctgc
     1141 ttcgtttcct gatggaagcg gcgttcgatc cggatgtgga ggagatcaag atcacgcagt
     1201 accgcgtagc cgaaaactcg gcggtgattc attcgctgat cagtgccgcg caaaacggaa
     1261 agaaggtgac ggtgttcgtc gagctgaaag cgcgtttcga tgaagagaac aacatgagta
     1321 cggccgaaaa gatgaaaaaa gccggcatca aaatcattta cagtattccg ggactcaagg
     1381 ttcatgcgaa ggtggctgtc attctgcgta aaggagaagc gacggatttt gcctatgtga
     1441 gtaccggcaa ttttaacgag aaaacggcga aaatctacgc cgacatggct ttgctgacat
     1501 gcaacaaggc gattgtcgac gatatcaacc gggtcttttc cgtgctggaa gggacgcttc
     1561 aggagccgca atttagttgt ttgcttgtcg cgcgtttcaa catggtgccg gaactgattc
     1621 gtatgattcg tcgcgagacg gaacatgtga aagccggcag gaagggtcga attgttttga
     1681 aaatgaatgg tctgcacgac cggagaatga tcgacgagct gtatcgtgcc ggttcggcgg
     1741 gtgtagagat cgacctgttg gtgcgcggta tctgctgtct ggtgcccgac cagccgtata
     1801 gtccgaatat ccgtgtgacg cggattgtag acatgtttct cgaacatgcg cgtatctggt
     1861 atttttataa cgatggggcg gaagatctgt atcttacctc gtcggactgg atgaaccgta
     1921 acctgaatcg tcgcatcgag acggctgctc ccgtgctgtc gccatccgtc aaacgtgaaa
     1981 tgatcgacat cctgcatatc cagttgcagg ataacgtcaa ggcttgccat atcgatgcgc
     2041 agttacggaa tgtcttcgtt cgtaacgacg cgccgcccat acgtgcccag caggctattt
     2101 acgactacat caaagcaaag accgagggct gttag
//