LOCUS BBa_K1217008 4926 bp DNA linear 23-Dec-2024 DEFINITION Localizing polyphosphate kinase (ppk1) into native Eut microcompartment (BBa_K311004) ACCESSION BBa_K1217008 VERSION BBa_K1217008.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 misc_feature 39..95 /label=Eut MCP-targeting signal sequence CDS 96..2147 /label=BBa_K1217002 CDS 39..2147 /label=BBa_K1217007 misc_feature 1..2147 /label=BBa_K1217005 promoter 2156..2279 /label=modified plac misc_feature 2295..2630 /label=Eut S promoter 2711..2834 /label=modified plac misc_feature 2850..3140 /label=EutM misc_feature 3241..3540 /label=EutN promoter 3621..3744 /label=modified plac misc_feature 3760..4419 /label=EutL misc_feature 4432..4926 /label=EutK misc_feature 2156..4926 /label=BBa_K311004 misc_feature 2156..4926 /label=BBa_K311004 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat ggatcaaaaa cagattgaag 61 aaattgtacg tagcgtgatg gcgtcaatgg gacagatgcc gcaatctgct cacattcttt 121 gccgcgaact gtcgctgttg gcgtttaacc gccgcgtgtt ggcgcaagcg caagacccgc 181 gcgtgccgct gttggaacga ctgcgctttt tgtgcatcgt gtcgtccaat ctggacgaat 241 tttttgaagt gcgcatggcg tatttgcgcc gtgaacaacg cgccaacccc acgcaagtgt 301 tccaatcggg acaaacgccc agcgaaacca tcacgctggt ggcgcaagaa gcgcacaaga 361 ttattgaaga gcaatatcaa atatttaacc aagaattgct gcccgcgctt gccgaggaaa 421 atattgtgtt ttaccgccgc aagcagtgga caagcgcgca gcgcgaatgg attgaagatt 481 atttcaaccg cgagctgctg ccgattctca cgccgattgg cttggatgcc tcgcatccgt 541 ttccgcgtcc gctgaataaa tcgttgaatt ttgtggtgga attggatggc aaagatgcgt 601 ttggacgcgc ttcgggcatg gcaatcgtgc aagcgccgcg cattttgccg cgtgcgattc 661 ggctgcctga aaatatttgc gaaggcaaag acggctttgt gtttttgtcg tccattgtgc 721 atgagtatgt gtacaagctg tttcgcggca tgacggtgaa aggctgccat caatttcgcc 781 taacgcgcga tagcgatttg acggtggaaa acgatgattt gagcaacctg cgcacggcgg 841 tgcaaaatga attgcgcgac cgcgattttg gcgacggagt gcggctggaa attgccgacc 901 aatgtcctga ttatattagc caatttttgc tggaacagtt taagctcacg cgcaatgagt 961 tgtatcaagt ggaaggtcct gtgaatttgg tgcggctgat gtcggtgccc gatatggtga 1021 accgtcccga tttgaaattc gcgccgttca cgccgcaata tccgcgcgcg ttgcgcaaat 1081 cggcatcgct gttggacgaa atcgctaagg gcgatatttt gctgcaccat ccgtatcaat 1141 cgtttgatcc gattgtgaaa tttatcaacg atgccacgcg cgatcctgat gtggtggcga 1201 ttaagatgac gatttaccgc acgggtagcc attccgagct ggcgcgcgct ttgatggcgg 1261 cggcgctggc gggcaagcaa gttaccgtgg tggtggaatt gatggcgcgt tttgacgaag 1321 cgaataatgt gtcgtgggca accaagctgg aagacgcggg cgcgcacgtg gtttatggcg 1381 tgtttggcta caaggtgcac gccaaaatgg cgttggtgat tcgccgcgaa caaggcagcc 1441 tgaaaaccta tgcgcatctg ggcacgggca attatcacca aggcacatcg cgcatctaca 1501 ccgatttcgg cttgctcacc gccaacacgg cgataacgcg cgacatcaac acgctattta 1561 tggaaatcac cggcttgggg caggcgaaca agctgaaaaa aatctaccaa agcccgttca 1621 cgctgcacaa aatgattatg gacagcatcg cgcgcgaaac cgaaaacgcg aaaaacggca 1681 agcctgcgcg gattatcgcc aagctcaatt cgctgattga gccgcaagtg atagaggcgt 1741 tgtatgccgc cagcgcggcg ggggtgcaga ttgatttgat tgtgcgtggc atgtgcgctt 1801 tgcgcccaca agtggcgggg ctgtcggaaa acatccgcgt gcgctccatt attgggcggc 1861 tgctggaaca cgcgcgggtt tattattttt acaacgatgg cgcggaaaac gtgtttattt 1921 ccagcgcgga ttggatgggg cgcaattttt tcagccgcat tgaaacctgc acgccgattg 1981 aatcgcccgc gctcaaacag cgcgtgatag aagagggctt actgctggcg ttgcgcgaca 2041 acgtgaaggc ttggcagatg cacggcgacg gcagctacac gcggattgtg gcggacggcg 2101 acgaggtggg cattcaagaa acgctgttgg cgcgatatac ggaatgatac tagagcccga 2161 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 2221 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcgtctagta 2281 gaaggaggag atctatgaat aaagaacgca ttattcagga atttgtgccg ggcaaacagg 2341 tcacgctggc gcatctcatt gcgcaccctg gtgaagaact ggcgaaaaag atcggcgttc 2401 ccgatgctgg cgcgattggc attatgactt taacgccggg ggaaacggcg atgatcgctg 2461 gcgatttagc gatgaaggct gcggatgtac acatcggttt tctcgataga tttagcggcg 2521 cgctggtgat ttatggcacc gtcggcgcag tagaagaagc gctattacag acggtcagcg 2581 ggctggggcg attattaaac tttaccctgt gtgagttaac aaaaagttaa gcggccgcct 2641 cgagcatcac catcaccatc actgattctc cttacgcatc tgtgcggtat ttcacaccgc 2701 atgtactaga cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 2761 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 2821 tgtgagcgtc tagtagaagg aggagatcta tggaagcatt aggaatgatt gaaacccggg 2881 gcctggttgc gctgattgag gcctccgatg cgatggtaaa agccgcgcgc gtgaagctgg 2941 tcggcgtgaa gcagattggc ggtggcctgt gtactgccat ggtgcgtggc gatgtggcgg 3001 cgtgcaaagc cgcaaccgat gctggcgccg ctgcggcgca gcgcattggc gagttggtct 3061 ccgtacacgt gattccacgc ccgcacggcg atctggaaga agtgttcccg atcagcttca 3121 aaggcgacag caacatttga tggtaggccg gataagacgc gcagcgtcgc catccggcat 3181 gaatgcgcgc cattgcctga tggtgcgcag attcaaggcc tacgacccaa cgggcaatcc 3241 atggaggcgg atatgaaact ggcagtcgtc acaggacaaa tcgtatgtac cgtccgccat 3301 cagggactgg cgcatgacaa attgctgatg gtggaaatga tcgatgccca gggcaacccc 3361 gacgggcagt gtgccgtcgc tatcgacagt atcggggcgg gaaccggaga gtgggtactg 3421 ctggtcagcg gcagttccgc acgccaggcg catcgcagcg aattatcacc ggtcgatctg 3481 tgcgtcattg gcatcgtcga tgaagtggtg gctggcggga aagtggtttt ccataaatag 3541 gcggccgcct cgagcatcac catcaccatc actgattctc cttacgcatc tgtgcggtat 3601 ttcacaccgc atgtactaga cccgactgga aagcgggcag tgagcgcaac gcaattaatg 3661 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 3721 tgtgtggaat tgtgagcgtc tagtagaagg aggagatcta tgcctgcatt agatttaatt 3781 cgaccttcag tgactgccat gcgcgtgatt gcctccgtga atgacggctt tgcgcgggaa 3841 cttaaattac cgccacatat acgtagtctc ggactcatca cggcagattc tgatgatgtg 3901 acctatattg ccgctgacga agcgacaaaa caggcgatgg tggaagtggt ctatggccgc 3961 tccctctatg ccggggcggc tcacgggccg tcgcctaccg ctggtgaggt gttgattatg 4021 ctgggcggac cgaacccggc ggaagtgcgc gccggtctgg atgcaatggt cgccagcatt 4081 gaaaacggcg cggcgttcca gtgggcgaac gatgcggaaa ataccgcatt cctggcgcat 4141 gtggtttcgc gtaccggctc gtacctctcg tctaccgcag gtatcgcgct gggcgatcca 4201 atggcctacc tggtggcgcc gccgctggaa gcgacattcg gcattgacgc ggcgatgaaa 4261 tccgctgacg ttcagctggt gacttacgtg ccgccgccgt cggaaaccaa ctattcggcc 4321 gcgttcttaa cgggaagcca ggccgcgtgt aaagccgcct gcaacgcctt taccgacgcc 4381 gtgctggata tcgcccgtaa ccctgtccag cgtgcgtaac ggaggctgcc aatgatcaat 4441 gccctgggat tactggaagt ggacggaatg gtcgccgccg tcgatgcggc ggatgctatg 4501 ctgaaagcgg ccaacgtgcg tttgctcagt catcaagtgc tcgaccctgg caggctcacg 4561 ctggtggtgg agggcgatct ggcggcgtgt cgtgcggcgc tggatgccgg aagcgctgcc 4621 gcgcagcgca caggccgtgt gattagtcgc aaagaaattg gtcgtccgga agaggacacc 4681 cagtggctga ttggcgggtt tgcgcgtgcg acaacgccga ctgagaaagc accccaggta 4741 cccgcaacgc ctgagttcgc tgaggcgtta ttggcgctgc tggcatcagt acgccaggga 4801 atgacggcgg gagaggttgc cgcgcatttt ggctggccgc tggagcaggc cagaaacgtg 4861 ctggaacaac tcttttctga cggagcgttg cgtaaacgca gtagtcgcta tcgcatcaaa 4921 aattaa //