LOCUS BBa_K1217009 4860 bp DNA linear 23-Dec-2024 DEFINITION Localizing polyphosphate kinase (ppk1) into Histag modified Eut microcompartment (BBa_K311004) ACCESSION BBa_K1217009 VERSION BBa_K1217009.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 misc_feature 39..95 /label=Eut MCP-targeting signal sequence CDS 96..2147 /label=BBa_K1217002 CDS 39..2147 /label=BBa_K1217007 misc_feature 1..2147 /label=BBa_K1217005 misc_feature 2176..2187 /label=B0034 promoter 2156..2175 /label=T7 promoter RBS 2179..2182 /label=RBS promoter 2156..2187 /label=BBa_K525998 CDS 2194..4860 /label=BBa_K1217012 misc_feature 2156..4860 /label=BBa_K1217015 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat ggatcaaaaa cagattgaag 61 aaattgtacg tagcgtgatg gcgtcaatgg gacagatgcc gcaatctgct cacattcttt 121 gccgcgaact gtcgctgttg gcgtttaacc gccgcgtgtt ggcgcaagcg caagacccgc 181 gcgtgccgct gttggaacga ctgcgctttt tgtgcatcgt gtcgtccaat ctggacgaat 241 tttttgaagt gcgcatggcg tatttgcgcc gtgaacaacg cgccaacccc acgcaagtgt 301 tccaatcggg acaaacgccc agcgaaacca tcacgctggt ggcgcaagaa gcgcacaaga 361 ttattgaaga gcaatatcaa atatttaacc aagaattgct gcccgcgctt gccgaggaaa 421 atattgtgtt ttaccgccgc aagcagtgga caagcgcgca gcgcgaatgg attgaagatt 481 atttcaaccg cgagctgctg ccgattctca cgccgattgg cttggatgcc tcgcatccgt 541 ttccgcgtcc gctgaataaa tcgttgaatt ttgtggtgga attggatggc aaagatgcgt 601 ttggacgcgc ttcgggcatg gcaatcgtgc aagcgccgcg cattttgccg cgtgcgattc 661 ggctgcctga aaatatttgc gaaggcaaag acggctttgt gtttttgtcg tccattgtgc 721 atgagtatgt gtacaagctg tttcgcggca tgacggtgaa aggctgccat caatttcgcc 781 taacgcgcga tagcgatttg acggtggaaa acgatgattt gagcaacctg cgcacggcgg 841 tgcaaaatga attgcgcgac cgcgattttg gcgacggagt gcggctggaa attgccgacc 901 aatgtcctga ttatattagc caatttttgc tggaacagtt taagctcacg cgcaatgagt 961 tgtatcaagt ggaaggtcct gtgaatttgg tgcggctgat gtcggtgccc gatatggtga 1021 accgtcccga tttgaaattc gcgccgttca cgccgcaata tccgcgcgcg ttgcgcaaat 1081 cggcatcgct gttggacgaa atcgctaagg gcgatatttt gctgcaccat ccgtatcaat 1141 cgtttgatcc gattgtgaaa tttatcaacg atgccacgcg cgatcctgat gtggtggcga 1201 ttaagatgac gatttaccgc acgggtagcc attccgagct ggcgcgcgct ttgatggcgg 1261 cggcgctggc gggcaagcaa gttaccgtgg tggtggaatt gatggcgcgt tttgacgaag 1321 cgaataatgt gtcgtgggca accaagctgg aagacgcggg cgcgcacgtg gtttatggcg 1381 tgtttggcta caaggtgcac gccaaaatgg cgttggtgat tcgccgcgaa caaggcagcc 1441 tgaaaaccta tgcgcatctg ggcacgggca attatcacca aggcacatcg cgcatctaca 1501 ccgatttcgg cttgctcacc gccaacacgg cgataacgcg cgacatcaac acgctattta 1561 tggaaatcac cggcttgggg caggcgaaca agctgaaaaa aatctaccaa agcccgttca 1621 cgctgcacaa aatgattatg gacagcatcg cgcgcgaaac cgaaaacgcg aaaaacggca 1681 agcctgcgcg gattatcgcc aagctcaatt cgctgattga gccgcaagtg atagaggcgt 1741 tgtatgccgc cagcgcggcg ggggtgcaga ttgatttgat tgtgcgtggc atgtgcgctt 1801 tgcgcccaca agtggcgggg ctgtcggaaa acatccgcgt gcgctccatt attgggcggc 1861 tgctggaaca cgcgcgggtt tattattttt acaacgatgg cgcggaaaac gtgtttattt 1921 ccagcgcgga ttggatgggg cgcaattttt tcagccgcat tgaaacctgc acgccgattg 1981 aatcgcccgc gctcaaacag cgcgtgatag aagagggctt actgctggcg ttgcgcgaca 2041 acgtgaaggc ttggcagatg cacggcgacg gcagctacac gcggattgtg gcggacggcg 2101 acgaggtggg cattcaagaa acgctgttgg cgcgatatac ggaatgatac tagagtaata 2161 cgactcacta tagggaaaga ggagaaatac tagatgcatc atcaccatca ccacggtagc 2221 ggcacgggta tgaataaaga acgcattatt caggaatttg tgccgggcaa acaggtcacg 2281 ctggcgcatc tcattgcgca ccctggtgaa gaactggcga aaaagatcgg cgttcccgat 2341 gctggcgcga ttggcattat gactttaacg ccgggggaaa cggcgatgat cgctggcgat 2401 ttagcgatga aggctgcgga tgtacacatc ggttttctcg atagatttag cggcgcgctg 2461 gtgatttatg gcaccgtcgg cgcagtagaa gaagcgctat tacagacggt cagcgggctg 2521 gggcgattat taaactttac cctgtgtgag ttaacaaaaa gttaagcggc cgcctcgagc 2581 atcaccatca ccatcactga ttctccttac gcatctgtgc ggtatttcac accgcatgta 2641 ctagacccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 2701 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 2761 gcgtctagta gaaggaggag atctatggaa gcattaggaa tgattgaaac ccggggcctg 2821 gttgcgctga ttgaggcctc cgatgcgatg gtaaaagccg cgcgcgtgaa gctggtcggc 2881 gtgaagcaga ttggcggtgg cctgtgtact gccatggtgc gtggcgatgt ggcggcgtgc 2941 aaagccgcaa ccgatgctgg cgccgctgcg gcgcagcgca ttggcgagtt ggtctccgta 3001 cacgtgattc cacgcccgca cggcgatctg gaagaagtgt tcccgatcag cttcaaaggc 3061 gacagcaaca tttgatggta ggccggataa gacgcgcagc gtcgccatcc ggcatgaatg 3121 cgcgccattg cctgatggtg cgcagattca aggcctacga cccaacgggc aatccatgga 3181 ggcggatatg aaactggcag tcgtcacagg acaaatcgta tgtaccgtcc gccatcaggg 3241 actggcgcat gacaaattgc tgatggtgga aatgatcgat gcccagggca accccgacgg 3301 gcagtgtgcc gtcgctatcg acagtatcgg ggcgggaacc ggagagtggg tactgctggt 3361 cagcggcagt tccgcacgcc aggcgcatcg cagcgaatta tcaccggtcg atctgtgcgt 3421 cattggcatc gtcgatgaag tggtggctgg cgggaaagtg gttttccata aataggcggc 3481 cgcctcgagc atcaccatca ccatcactga ttctccttac gcatctgtgc ggtatttcac 3541 accgcatgta ctagacccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt 3601 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt 3661 ggaattgtga gcgtctagta gaaggaggag atctatgcct gcattagatt taattcgacc 3721 ttcagtgact gccatgcgcg tgattgcctc cgtgaatgac ggctttgcgc gggaacttaa 3781 attaccgcca catatacgta gtctcggact catcacggca gattctgatg atgtgaccta 3841 tattgccgct gacgaagcga caaaacaggc gatggtggaa gtggtctatg gccgctccct 3901 ctatgccggg gcggctcacg ggccgtcgcc taccgctggt gaggtgttga ttatgctggg 3961 cggaccgaac ccggcggaag tgcgcgccgg tctggatgca atggtcgcca gcattgaaaa 4021 cggcgcggcg ttccagtggg cgaacgatgc ggaaaatacc gcattcctgg cgcatgtggt 4081 ttcgcgtacc ggctcgtacc tctcgtctac cgcaggtatc gcgctgggcg atccaatggc 4141 ctacctggtg gcgccgccgc tggaagcgac attcggcatt gacgcggcga tgaaatccgc 4201 tgacgttcag ctggtgactt acgtgccgcc gccgtcggaa accaactatt cggccgcgtt 4261 cttaacggga agccaggccg cgtgtaaagc cgcctgcaac gcctttaccg acgccgtgct 4321 ggatatcgcc cgtaaccctg tccagcgtgc gtaacggagg ctgccaatga tcaatgccct 4381 gggattactg gaagtggacg gaatggtcgc cgccgtcgat gcggcggatg ctatgctgaa 4441 agcggccaac gtgcgtttgc tcagtcatca agtgctcgac cctggcaggc tcacgctggt 4501 ggtggagggc gatctggcgg cgtgtcgtgc ggcgctggat gccggaagcg ctgccgcgca 4561 gcgcacaggc cgtgtgatta gtcgcaaaga aattggtcgt ccggaagagg acacccagtg 4621 gctgattggc gggtttgcgc gtgcgacaac gccgactgag aaagcacccc aggtacccgc 4681 aacgcctgag ttcgctgagg cgttattggc gctgctggca tcagtacgcc agggaatgac 4741 ggcgggagag gttgccgcgc attttggctg gccgctggag caggccagaa acgtgctgga 4801 acaactcttt tctgacggag cgttgcgtaa acgcagtagt cgctatcgca tcaaaaataa //