LOCUS       BBa_K1217011            2565 bp    DNA     linear       14-Jun-2024
DEFINITION  pT7-ppk1(K.oralis)+HisTag_EutS
ACCESSION   BBa_K1217011
VERSION     BBa_K1217011.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     misc_feature    39..95
                     /label=Eut MCP-targeting signal sequence
     CDS             96..2147
                     /label=BBa_K1217002
     CDS             39..2147
                     /label=BBa_K1217007
     misc_feature    1..2147
                     /label=BBa_K1217005
     misc_feature    2176..2187
                     /label=B0034
     promoter        2156..2175
                     /label=T7 promoter
     RBS             2179..2182
                     /label=RBS
     promoter        2156..2187
                     /label=BBa_K525998
     misc_feature    2197..2214
                     /label=6 His
     misc_feature    2215..2229
                     /label=linker
     CDS             2230..2565
                     /label=K1217013
     CDS             2194..2565
                     /label=BBa_K1217014
     misc_feature    2156..2565
                     /label=BBa_K1217017
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat ggatcaaaaa cagattgaag
       61 aaattgtacg tagcgtgatg gcgtcaatgg gacagatgcc gcaatctgct cacattcttt
      121 gccgcgaact gtcgctgttg gcgtttaacc gccgcgtgtt ggcgcaagcg caagacccgc
      181 gcgtgccgct gttggaacga ctgcgctttt tgtgcatcgt gtcgtccaat ctggacgaat
      241 tttttgaagt gcgcatggcg tatttgcgcc gtgaacaacg cgccaacccc acgcaagtgt
      301 tccaatcggg acaaacgccc agcgaaacca tcacgctggt ggcgcaagaa gcgcacaaga
      361 ttattgaaga gcaatatcaa atatttaacc aagaattgct gcccgcgctt gccgaggaaa
      421 atattgtgtt ttaccgccgc aagcagtgga caagcgcgca gcgcgaatgg attgaagatt
      481 atttcaaccg cgagctgctg ccgattctca cgccgattgg cttggatgcc tcgcatccgt
      541 ttccgcgtcc gctgaataaa tcgttgaatt ttgtggtgga attggatggc aaagatgcgt
      601 ttggacgcgc ttcgggcatg gcaatcgtgc aagcgccgcg cattttgccg cgtgcgattc
      661 ggctgcctga aaatatttgc gaaggcaaag acggctttgt gtttttgtcg tccattgtgc
      721 atgagtatgt gtacaagctg tttcgcggca tgacggtgaa aggctgccat caatttcgcc
      781 taacgcgcga tagcgatttg acggtggaaa acgatgattt gagcaacctg cgcacggcgg
      841 tgcaaaatga attgcgcgac cgcgattttg gcgacggagt gcggctggaa attgccgacc
      901 aatgtcctga ttatattagc caatttttgc tggaacagtt taagctcacg cgcaatgagt
      961 tgtatcaagt ggaaggtcct gtgaatttgg tgcggctgat gtcggtgccc gatatggtga
     1021 accgtcccga tttgaaattc gcgccgttca cgccgcaata tccgcgcgcg ttgcgcaaat
     1081 cggcatcgct gttggacgaa atcgctaagg gcgatatttt gctgcaccat ccgtatcaat
     1141 cgtttgatcc gattgtgaaa tttatcaacg atgccacgcg cgatcctgat gtggtggcga
     1201 ttaagatgac gatttaccgc acgggtagcc attccgagct ggcgcgcgct ttgatggcgg
     1261 cggcgctggc gggcaagcaa gttaccgtgg tggtggaatt gatggcgcgt tttgacgaag
     1321 cgaataatgt gtcgtgggca accaagctgg aagacgcggg cgcgcacgtg gtttatggcg
     1381 tgtttggcta caaggtgcac gccaaaatgg cgttggtgat tcgccgcgaa caaggcagcc
     1441 tgaaaaccta tgcgcatctg ggcacgggca attatcacca aggcacatcg cgcatctaca
     1501 ccgatttcgg cttgctcacc gccaacacgg cgataacgcg cgacatcaac acgctattta
     1561 tggaaatcac cggcttgggg caggcgaaca agctgaaaaa aatctaccaa agcccgttca
     1621 cgctgcacaa aatgattatg gacagcatcg cgcgcgaaac cgaaaacgcg aaaaacggca
     1681 agcctgcgcg gattatcgcc aagctcaatt cgctgattga gccgcaagtg atagaggcgt
     1741 tgtatgccgc cagcgcggcg ggggtgcaga ttgatttgat tgtgcgtggc atgtgcgctt
     1801 tgcgcccaca agtggcgggg ctgtcggaaa acatccgcgt gcgctccatt attgggcggc
     1861 tgctggaaca cgcgcgggtt tattattttt acaacgatgg cgcggaaaac gtgtttattt
     1921 ccagcgcgga ttggatgggg cgcaattttt tcagccgcat tgaaacctgc acgccgattg
     1981 aatcgcccgc gctcaaacag cgcgtgatag aagagggctt actgctggcg ttgcgcgaca
     2041 acgtgaaggc ttggcagatg cacggcgacg gcagctacac gcggattgtg gcggacggcg
     2101 acgaggtggg cattcaagaa acgctgttgg cgcgatatac ggaatgatac tagagtaata
     2161 cgactcacta tagggaaaga ggagaaatac tagatgcatc atcaccatca ccacggtagc
     2221 ggcacgggta tgaataaaga acgcattatt caggaatttg tgccgggcaa acaggtcacg
     2281 ctggcgcatc tcattgcgca ccctggtgaa gaactggcga aaaagatcgg cgttcccgat
     2341 gctggcgcga ttggcattat gactttaacg ccgggggaaa cggcgatgat cgctggcgat
     2401 ttagcgatga aggctgcgga tgtacacatc ggttttctcg atagatttag cggcgcgctg
     2461 gtgatttatg gcaccgtcgg cgcagtagaa gaagcgctat tacagacggt cagcgggctg
     2521 gggcgattat taaactttac cctgtgtgag ttaacaaaaa gttaa
//