LOCUS BBa_K1217021 3580 bp DNA linear 23-Dec-2024 DEFINITION pT7-Sig_RFP+EutSMNLK ACCESSION BBa_K1217021 VERSION BBa_K1217021.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 misc_feature 39..95 /label=Eut MCP-targeting sequence CDS 96..801 /label=BBa_E1010 CDS 39..801 /label=BBa_K1217018 misc_feature 1..801 /label=BBa_K1217019 promoter 810..933 /label=modified plac misc_feature 949..1284 /label=Eut S promoter 1365..1488 /label=modified plac misc_feature 1504..1794 /label=EutM misc_feature 1895..2194 /label=EutN promoter 2275..2398 /label=modified plac misc_feature 2414..3073 /label=EutL misc_feature 3086..3580 /label=EutK misc_feature 810..3580 /label=BBa_K311004 misc_feature 810..3580 /label=BBa_K311004 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat ggatcaaaaa cagattgaag 61 aaattgtacg tagcgtgatg gcgtcaatgg gacagatggc ttcctccgaa gacgttatca 121 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa 181 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta 241 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt 301 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg 361 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc 421 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact 481 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac 541 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag 601 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc 661 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca 721 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct 781 gatagtgcta gtgtagatcg ctactagagc ccgactggaa agcgggcagt gagcgcaacg 841 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 901 ctcgtatgtt gtgtggaatt gtgagcgtct agtagaagga ggagatctat gaataaagaa 961 cgcattattc aggaatttgt gccgggcaaa caggtcacgc tggcgcatct cattgcgcac 1021 cctggtgaag aactggcgaa aaagatcggc gttcccgatg ctggcgcgat tggcattatg 1081 actttaacgc cgggggaaac ggcgatgatc gctggcgatt tagcgatgaa ggctgcggat 1141 gtacacatcg gttttctcga tagatttagc ggcgcgctgg tgatttatgg caccgtcggc 1201 gcagtagaag aagcgctatt acagacggtc agcgggctgg ggcgattatt aaactttacc 1261 ctgtgtgagt taacaaaaag ttaagcggcc gcctcgagca tcaccatcac catcactgat 1321 tctccttacg catctgtgcg gtatttcaca ccgcatgtac tagacccgac tggaaagcgg 1381 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac 1441 actttatgct tccggctcgt atgttgtgtg gaattgtgag cgtctagtag aaggaggaga 1501 tctatggaag cattaggaat gattgaaacc cggggcctgg ttgcgctgat tgaggcctcc 1561 gatgcgatgg taaaagccgc gcgcgtgaag ctggtcggcg tgaagcagat tggcggtggc 1621 ctgtgtactg ccatggtgcg tggcgatgtg gcggcgtgca aagccgcaac cgatgctggc 1681 gccgctgcgg cgcagcgcat tggcgagttg gtctccgtac acgtgattcc acgcccgcac 1741 ggcgatctgg aagaagtgtt cccgatcagc ttcaaaggcg acagcaacat ttgatggtag 1801 gccggataag acgcgcagcg tcgccatccg gcatgaatgc gcgccattgc ctgatggtgc 1861 gcagattcaa ggcctacgac ccaacgggca atccatggag gcggatatga aactggcagt 1921 cgtcacagga caaatcgtat gtaccgtccg ccatcaggga ctggcgcatg acaaattgct 1981 gatggtggaa atgatcgatg cccagggcaa ccccgacggg cagtgtgccg tcgctatcga 2041 cagtatcggg gcgggaaccg gagagtgggt actgctggtc agcggcagtt ccgcacgcca 2101 ggcgcatcgc agcgaattat caccggtcga tctgtgcgtc attggcatcg tcgatgaagt 2161 ggtggctggc gggaaagtgg ttttccataa ataggcggcc gcctcgagca tcaccatcac 2221 catcactgat tctccttacg catctgtgcg gtatttcaca ccgcatgtac tagacccgac 2281 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 2341 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cgtctagtag 2401 aaggaggaga tctatgcctg cattagattt aattcgacct tcagtgactg ccatgcgcgt 2461 gattgcctcc gtgaatgacg gctttgcgcg ggaacttaaa ttaccgccac atatacgtag 2521 tctcggactc atcacggcag attctgatga tgtgacctat attgccgctg acgaagcgac 2581 aaaacaggcg atggtggaag tggtctatgg ccgctccctc tatgccgggg cggctcacgg 2641 gccgtcgcct accgctggtg aggtgttgat tatgctgggc ggaccgaacc cggcggaagt 2701 gcgcgccggt ctggatgcaa tggtcgccag cattgaaaac ggcgcggcgt tccagtgggc 2761 gaacgatgcg gaaaataccg cattcctggc gcatgtggtt tcgcgtaccg gctcgtacct 2821 ctcgtctacc gcaggtatcg cgctgggcga tccaatggcc tacctggtgg cgccgccgct 2881 ggaagcgaca ttcggcattg acgcggcgat gaaatccgct gacgttcagc tggtgactta 2941 cgtgccgccg ccgtcggaaa ccaactattc ggccgcgttc ttaacgggaa gccaggccgc 3001 gtgtaaagcc gcctgcaacg cctttaccga cgccgtgctg gatatcgccc gtaaccctgt 3061 ccagcgtgcg taacggaggc tgccaatgat caatgccctg ggattactgg aagtggacgg 3121 aatggtcgcc gccgtcgatg cggcggatgc tatgctgaaa gcggccaacg tgcgtttgct 3181 cagtcatcaa gtgctcgacc ctggcaggct cacgctggtg gtggagggcg atctggcggc 3241 gtgtcgtgcg gcgctggatg ccggaagcgc tgccgcgcag cgcacaggcc gtgtgattag 3301 tcgcaaagaa attggtcgtc cggaagagga cacccagtgg ctgattggcg ggtttgcgcg 3361 tgcgacaacg ccgactgaga aagcacccca ggtacccgca acgcctgagt tcgctgaggc 3421 gttattggcg ctgctggcat cagtacgcca gggaatgacg gcgggagagg ttgccgcgca 3481 ttttggctgg ccgctggagc aggccagaaa cgtgctggaa caactctttt ctgacggagc 3541 gttgcgtaaa cgcagtagtc gctatcgcat caaaaattaa //