LOCUS BBa_K1217023 3514 bp DNA linear 23-Dec-2024 DEFINITION pT7-Sig_RFP+HisTag_EutSMNLK ACCESSION BBa_K1217023 VERSION BBa_K1217023.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 misc_feature 39..95 /label=Eut MCP-targeting sequence CDS 96..801 /label=BBa_E1010 CDS 39..801 /label=BBa_K1217018 misc_feature 1..801 /label=BBa_K1217019 misc_feature 830..841 /label=B0034 promoter 810..829 /label=T7 promoter RBS 833..836 /label=RBS promoter 810..841 /label=BBa_K525998 CDS 848..3514 /label=BBa_K1217012 misc_feature 810..3514 /label=BBa_K1217015 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat ggatcaaaaa cagattgaag 61 aaattgtacg tagcgtgatg gcgtcaatgg gacagatggc ttcctccgaa gacgttatca 121 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa 181 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta 241 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt 301 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg 361 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc 421 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact 481 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac 541 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag 601 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc 661 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca 721 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct 781 gatagtgcta gtgtagatcg ctactagagt aatacgactc actataggga aagaggagaa 841 atactagatg catcatcacc atcaccacgg tagcggcacg ggtatgaata aagaacgcat 901 tattcaggaa tttgtgccgg gcaaacaggt cacgctggcg catctcattg cgcaccctgg 961 tgaagaactg gcgaaaaaga tcggcgttcc cgatgctggc gcgattggca ttatgacttt 1021 aacgccgggg gaaacggcga tgatcgctgg cgatttagcg atgaaggctg cggatgtaca 1081 catcggtttt ctcgatagat ttagcggcgc gctggtgatt tatggcaccg tcggcgcagt 1141 agaagaagcg ctattacaga cggtcagcgg gctggggcga ttattaaact ttaccctgtg 1201 tgagttaaca aaaagttaag cggccgcctc gagcatcacc atcaccatca ctgattctcc 1261 ttacgcatct gtgcggtatt tcacaccgca tgtactagac ccgactggaa agcgggcagt 1321 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt 1381 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcgtct agtagaagga ggagatctat 1441 ggaagcatta ggaatgattg aaacccgggg cctggttgcg ctgattgagg cctccgatgc 1501 gatggtaaaa gccgcgcgcg tgaagctggt cggcgtgaag cagattggcg gtggcctgtg 1561 tactgccatg gtgcgtggcg atgtggcggc gtgcaaagcc gcaaccgatg ctggcgccgc 1621 tgcggcgcag cgcattggcg agttggtctc cgtacacgtg attccacgcc cgcacggcga 1681 tctggaagaa gtgttcccga tcagcttcaa aggcgacagc aacatttgat ggtaggccgg 1741 ataagacgcg cagcgtcgcc atccggcatg aatgcgcgcc attgcctgat ggtgcgcaga 1801 ttcaaggcct acgacccaac gggcaatcca tggaggcgga tatgaaactg gcagtcgtca 1861 caggacaaat cgtatgtacc gtccgccatc agggactggc gcatgacaaa ttgctgatgg 1921 tggaaatgat cgatgcccag ggcaaccccg acgggcagtg tgccgtcgct atcgacagta 1981 tcggggcggg aaccggagag tgggtactgc tggtcagcgg cagttccgca cgccaggcgc 2041 atcgcagcga attatcaccg gtcgatctgt gcgtcattgg catcgtcgat gaagtggtgg 2101 ctggcgggaa agtggttttc cataaatagg cggccgcctc gagcatcacc atcaccatca 2161 ctgattctcc ttacgcatct gtgcggtatt tcacaccgca tgtactagac ccgactggaa 2221 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 2281 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcgtct agtagaagga 2341 ggagatctat gcctgcatta gatttaattc gaccttcagt gactgccatg cgcgtgattg 2401 cctccgtgaa tgacggcttt gcgcgggaac ttaaattacc gccacatata cgtagtctcg 2461 gactcatcac ggcagattct gatgatgtga cctatattgc cgctgacgaa gcgacaaaac 2521 aggcgatggt ggaagtggtc tatggccgct ccctctatgc cggggcggct cacgggccgt 2581 cgcctaccgc tggtgaggtg ttgattatgc tgggcggacc gaacccggcg gaagtgcgcg 2641 ccggtctgga tgcaatggtc gccagcattg aaaacggcgc ggcgttccag tgggcgaacg 2701 atgcggaaaa taccgcattc ctggcgcatg tggtttcgcg taccggctcg tacctctcgt 2761 ctaccgcagg tatcgcgctg ggcgatccaa tggcctacct ggtggcgccg ccgctggaag 2821 cgacattcgg cattgacgcg gcgatgaaat ccgctgacgt tcagctggtg acttacgtgc 2881 cgccgccgtc ggaaaccaac tattcggccg cgttcttaac gggaagccag gccgcgtgta 2941 aagccgcctg caacgccttt accgacgccg tgctggatat cgcccgtaac cctgtccagc 3001 gtgcgtaacg gaggctgcca atgatcaatg ccctgggatt actggaagtg gacggaatgg 3061 tcgccgccgt cgatgcggcg gatgctatgc tgaaagcggc caacgtgcgt ttgctcagtc 3121 atcaagtgct cgaccctggc aggctcacgc tggtggtgga gggcgatctg gcggcgtgtc 3181 gtgcggcgct ggatgccgga agcgctgccg cgcagcgcac aggccgtgtg attagtcgca 3241 aagaaattgg tcgtccggaa gaggacaccc agtggctgat tggcgggttt gcgcgtgcga 3301 caacgccgac tgagaaagca ccccaggtac ccgcaacgcc tgagttcgct gaggcgttat 3361 tggcgctgct ggcatcagta cgccagggaa tgacggcggg agaggttgcc gcgcattttg 3421 gctggccgct ggagcaggcc agaaacgtgc tggaacaact cttttctgac ggagcgttgc 3481 gtaaacgcag tagtcgctat cgcatcaaaa ataa //