LOCUS BBa_K1218013 5080 bp DNA linear 26-Nov-2024 DEFINITION dCas9 Repressor ACCESSION BBa_K1218013 VERSION BBa_K1218013.1 FEATURES Location/Qualifiers ORIGIN 1 ttacgaaatc atcctgtgga gcttagtagg tttagcaaga tggcagcgcc taaatgtaga 61 atgataaaag gattaagaga ttaatttccc taaaaatgat aaaacaagcg ttttgaaagc 121 gcttgttttt ttggtttgca gtcagagtag aatagaagta tcaaaaaaag caccgactcg 181 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat gctgttttga 241 atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag 301 aaatttatca gccataaaac aatacttaat actatagaat gataacaaaa taaactactt 361 tttaaaagaa ttttgtgtta taatctattt attattaagt attgggtaat attttttgaa 421 gagatatttt gaaaaagaaa aattaaagca tattaaacta atttcggagg tcattaaaac 481 tattattgaa atcatcaaac tcattatgga tttaatttaa actttttatt ttaggaggca 541 aaaatggata agaaatactc aataggctta gctatcggca caaatagcgt cggatgggcg 601 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac 661 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg 721 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt 781 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat 841 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt 901 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga 961 aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg 1021 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt 1081 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac 1141 cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca 1201 agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg 1261 aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca 1321 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg 1381 gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct 1441 attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct 1501 tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt 1561 cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat 1621 gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt 1681 ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg 1741 cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt gggtgagctg 1801 catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag 1861 attgaaaaaa tcttgacttt tcgtattcct tattatgttg gtccattggc gcgtggcaat 1921 agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa 1981 gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat 2041 aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg 2101 gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt 2161 ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta 2221 accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa 2281 atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa 2341 attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt 2401 gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat 2461 gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg 2521 ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata 2581 ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat 2641 gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt 2701 ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag 2761 actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc 2821 gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag 2881 cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat 2941 cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga 3001 agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat 3061 gccattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt 3121 tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg 3181 aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat 3241 ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc 3301 caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg 3361 aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa 3421 tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac 3481 aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag 3541 aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt 3601 aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac 3661 tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa 3721 cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat 3781 tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa 3841 gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt 3901 attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta 3961 gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc 4021 gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt 4081 gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct 4141 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa 4201 ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct 4261 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg 4321 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt 4381 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac 4441 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga 4501 gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca 4561 aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc 4621 attgatttga gtcagctagg aggtgactga agtatatttt agatgaagat tatttcttaa 4681 taactaaaaa tatggtataa tactcttaat aaatgcagta atacaggggc ttttcaagac 4741 tgaagtctag ctgagacaaa tagtgcgatt acgaaatttt ttagacaaaa atagtctacg 4801 aggttttaga gctatgctgt tttgaatggt cccaaaactg agaccagtct cggaagctca 4861 aaggtctcgt tttagagcta tgctgttttg aatggtccca aaacttcagc acactgagac 4921 ttgttgagtt ccatgtttta gagctatgct gttttgaatg gactccattc aacattgccg 4981 atgataactt gagaaagagg gttaatacca gcagtcggat accttcctat tctttctgtt 5041 aaagcgtttt catgttataa taggcaaaag aagagtagtg //