BBa_K1218024
1
BBa_K1218024
spo0A + blue chromoprotein
2013-09-16T11:00:00Z
2015-05-08T01:09:42Z
This part was synthesized by DNA 2.0.
This composite part contains the promoter for spo0A (a sporulation regulator: BBa_K1218021), a ribosome binding site (BBa_B0034), and a blue chromoprotein developed by Uppsala (aeBlue: BBa_K864403).
false
false
_1532_
0
17531
9
Not in stock
false
None
false
Evie Pless
component2348889
1
BBa_B0034
component2348895
1
BBa_K864403
component2348887
1
BBa_K1218021
annotation2348889
1
BBa_B0034
range2348889
1
68
79
annotation2348895
1
BBa_K864403
range2348895
1
88
847
annotation2348887
1
BBa_K1218021
range2348887
1
1
59
BBa_K1218021
1
BBa_K1218021
spo0A promoter
2013-09-03T11:00:00Z
2015-05-08T01:09:42Z
The promoter was made from oligos ordered from Elim.
This is a basic part: the spo0A promoter. spo0A regulates sporulation in Bacillus subtilis.
false
false
_1532_
0
17531
9
In stock
false
None.
false
Evie Pless
BBa_J23110
1
BBa_J23110
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K864401
1
aeBlue
aeBlue blue chromoprotein
2012-09-24T11:00:00Z
2015-05-08T01:13:37Z
Source
aeBlue
false
false
_1124_
0
9827
9
It's complicated
true
Synthesized and codon optimized for E. coli by Bioneer Corp.
false
Erik Lundin
annotation2197206
1
aeBlue
range2197206
1
1
699
BBa_K864403
1
aeBlue
J23110-B0034-aeBlue (chromoprotein) (E.coli optimized)
2012-09-24T11:00:00Z
2015-05-08T01:13:37Z
The protein was discovered, extracted and analyzed in (Shkrob, 2005). It was synthesized by Bioneer Corporation, codon optimized for E.coli.
aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.
This part is a functionally active variant of BBa_K864401.
false
false
_1124_
0
14206
9
In stock
true
The promoter J23110 is sufficiently strong to yield expression levels making the blue protein clearly visible and eventually opaque.
false
Olle Nordesj??
component2198623
1
BBa_J23110
component2198627
1
BBa_K864401
component2198625
1
BBa_B0034
annotation2198623
1
BBa_J23110
range2198623
1
1
35
annotation2198625
1
BBa_B0034
range2198625
1
44
55
annotation2198627
1
BBa_K864401
range2198627
1
62
760
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K864403_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa
BBa_K1218024_sequence
1
cactaatattggtgattatgatttttttagagggtatatagcggttttgtcgaatgtaatactagagaaagaggagaaatactagagtttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1218021_sequence
1
cactaatattggtgattatgatttttttagagggtatatagcggttttgtcgaatgtaa
BBa_J23110_sequence
1
tttacggctagctcagtcctaggtacaatgctagc
BBa_K864401_sequence
1
atggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z