BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1218021 1 BBa_K1218021 spo0A promoter 2013-09-03T11:00:00Z 2015-05-08T01:09:42Z The promoter was made from oligos ordered from Elim. This is a basic part: the spo0A promoter. spo0A regulates sporulation in Bacillus subtilis. false false _1532_ 0 17531 9 In stock false None. false Evie Pless BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1218024 1 BBa_K1218024 spo0A + blue chromoprotein 2013-09-16T11:00:00Z 2015-05-08T01:09:42Z This part was synthesized by DNA 2.0. This composite part contains the promoter for spo0A (a sporulation regulator: BBa_K1218021), a ribosome binding site (BBa_B0034), and a blue chromoprotein developed by Uppsala (aeBlue: BBa_K864403). false false _1532_ 0 17531 9 Not in stock false None false Evie Pless component2348889 1 BBa_B0034 component2348895 1 BBa_K864403 component2348887 1 BBa_K1218021 annotation2348889 1 BBa_B0034 range2348889 1 68 79 annotation2348895 1 BBa_K864403 range2348895 1 88 847 annotation2348887 1 BBa_K1218021 range2348887 1 1 59 BBa_K864403 1 aeBlue J23110-B0034-aeBlue (chromoprotein) (E.coli optimized) 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z The protein was discovered, extracted and analyzed in (Shkrob, 2005). It was synthesized by Bioneer Corporation, codon optimized for E.coli. aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities. This part is a functionally active variant of BBa_K864401. false false _1124_ 0 14206 9 In stock true The promoter J23110 is sufficiently strong to yield expression levels making the blue protein clearly visible and eventually opaque. false Olle Nordesj?? component2198625 1 BBa_B0034 component2198627 1 BBa_K864401 component2198623 1 BBa_J23110 annotation2198627 1 BBa_K864401 range2198627 1 62 760 annotation2198625 1 BBa_B0034 range2198625 1 44 55 annotation2198623 1 BBa_J23110 range2198623 1 1 35 BBa_K864401 1 aeBlue aeBlue blue chromoprotein 2012-09-24T11:00:00Z 2015-05-08T01:13:37Z Source aeBlue false false _1124_ 0 9827 9 It's complicated true Synthesized and codon optimized for E. coli by Bioneer Corp. false Erik Lundin annotation2197206 1 aeBlue range2197206 1 1 699 BBa_K864403_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa BBa_K1218024_sequence 1 cactaatattggtgattatgatttttttagagggtatatagcggttttgtcgaatgtaatactagagaaagaggagaaatactagagtttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1218021_sequence 1 cactaatattggtgattatgatttttttagagggtatatagcggttttgtcgaatgtaa BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc BBa_K864401_sequence 1 atggcttcactggttaaaaaagacatgtgcatcaaaatgacgatggaaggaacagtaaacggtcaccatttcaagtgtgtaggagaaggcgaaggcaaaccatttgaagggacccaggtggaaaagatacgcatcactgaaggtgggcccttaccatttgcgtatgatattttggccccttgttgcatgtatggcagtaaaaccttcattaagcatgtgtcgggtattccggattactttaaggagtcttttcctgagggctttacctgggaaagaacacaaatcttcgaggatggcggctatctcaccatacaccaggacacgagccttcagggtaataattttattttcaaagttaatgtcatcggtgccaacttccctgcaaacggtcccgtgatgcagaaaaaaacagctggatgggaaccgtgcgttgagatgctttatccgcgggacggcgtcctgtgtggtcagagcctgatggccctgaaatgcactgatggcaatcatctgacgtcccacctgcgcactacctatcgttctcgcaagccatccaatgcagttaacatgccggaatttcattttggggatcatcgcattgagattttgaaagctgaacaaggtaaattttatgaacaatacgagtcagcggtggcccgttactgtgaggcggcaccgagtaaattagggcatcactaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z