BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K121017 1 BBa_K121017 promoterless gfp 2008-10-25T11:00:00Z 2015-05-08T01:09:41Z BBa_I7100 Released HQ 2013 Restriction sites of Sal I, Bam HI, Bgl II, and Mlu I in front of strong RBS and gfp false false _220_ 0 765 76 In stock false We deleted the promoter of BBa_I7100 and inserted newly designed prefix and suffix each of which has 2 restriction-enzyme-sites(prefix: SalI and BamHI,surfix: BglII and MulI) But we did not insert new promoter. false Shotaro Ayukawa component1990496 1 BBa_B0010 component1990493 1 BBa_K121016 component1990495 1 BBa_E0040 component1990498 1 BBa_B0012 annotation1990495 1 BBa_E0040 range1990495 1 71 790 annotation1990493 1 BBa_K121016 range1990493 1 1 64 annotation1990498 1 BBa_B0012 range1990498 1 887 927 annotation1990496 1 BBa_B0010 range1990496 1 799 878 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K121016 1 BBa_K121016 Tokyo Standard prefix suffix RBS 2008-10-25T11:00:00Z 2015-05-08T01:09:41Z RBS is from BBa_0030 Restriction sites of Sal I, Bam HI, Bgl II, and Mlu I in front of strong RBS false false _220_ 0 765 76 Not in stock false We designed new prefix(Sal I and Bam HI) and suffix(Bgl II and Mul I) for insertions of promoters and regulator binding sites in front of RBS. false Shotaro Ayukawa annotation1988216 1 B0030 range1988216 1 50 64 annotation1988248 1 Bgl II range1988248 1 26 31 annotation1988246 1 Sal I range1988246 1 1 6 annotation1988250 1 Mlu I range1988250 1 41 46 annotation1988247 1 Bam HI range1988247 1 15 20 BBa_K121014 1 BBa_K121014 promoter (lambda cI regulated) 2008-10-15T11:00:00Z 2015-05-08T01:09:41Z na promoter (lambda cI regulated) false false _220_ 0 3180 9 Not in stock false na false Masashi Kajita annotation1981851 1 -35 range1981851 1 47 52 annotation1981850 1 -10 range1981850 1 70 75 annotation1981849 1 OL1 range1981849 1 57 73 annotation1981848 1 OL2 range1981848 1 33 49 annotation1981846 1 K121014 range1981846 1 1 90 annotation1981847 1 OL3 range1981847 1 13 29 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K121998 1 BBa_K121998 test 2008-10-25T11:00:00Z 2015-05-08T01:09:42Z test test false false _220_ 0 765 76 It's complicated false test false Shotaro Ayukawa component2222217 1 BBa_K121014 component2222232 1 BBa_K121017 annotation2222217 1 BBa_K121014 range2222217 1 1 90 annotation2222232 1 BBa_K121017 range2222232 1 99 1025 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K121014_sequence 1 gcatgcacagataaccatctgcggtgataaattatctctggcggtgttgacataaataccactggcggttataatgagcacatcagcagg BBa_K121998_sequence 1 gcatgcacagataaccatctgcggtgataaattatctctggcggtgttgacataaataccactggcggttataatgagcacatcagcaggtactagaggtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K121016_sequence 1 gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K121017_sequence 1 gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z