LOCUS       BBa_K1222001            1297 bp    DNA     linear       22-Dec-2024
DEFINITION  pGFP (T7 promoter+lac operator+GFP antisense"3"+T7 terminator+Lac 
            promoter+LacI)
ACCESSION   BBa_K1222001
VERSION     BBa_K1222001.1  
FEATURES             Location/Qualifiers
     promoter        1..23
                     /label=BBa_I719005
     misc_feature    32..96
                     /label=BBa_K1222985
     misc_feature    105..152
                     /label=T7 terminator
     terminator      105..152
                     /label=BBa_K731721
     misc_feature    161..197
                     /label=P(Lac) IQ
     promoter        163..168
                     /label=-35
     promoter        186..191
                     /label=-10
     promoter        161..197
                     /label=BBa_I14032
     CDS             206..1297
                     /label=BBa_K1222003
ORIGIN
        1 taatacgact cactataggg agatactaga gggaattgtg agcggataac aattcctcga
       61 ttctattaac aagggtatca ccttcaaact tgactttact agagctagca taaccccttg
      121 gggcctctaa acgggtcttg aggggttttt tgtactagag tggtgcaaaa cctttcgcgg
      181 tatggcatga tagcgcctac tagaggtggt gaatgtgaaa ccagtaacgt tatacgatgt
      241 cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca
      301 cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc
      361 caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc
      421 cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca
      481 actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc
      541 ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga
      601 tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga
      661 tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact
      721 gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt
      781 aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca
      841 aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac
      901 catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat
      961 ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc
     1021 ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat
     1081 caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca
     1141 gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac
     1201 cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     1261 ggcacgacag gtttcccgac tggaaagcgg gcagtga
//