BBa_K1222001 1 BBa_K1222001 pGFP (T7 promoter+lac operator+GFP antisense"3"+T7 terminator+Lac promoter+LacI) 2013-09-23T11:00:00Z 2015-05-08T01:09:43Z This part is composed of previous igem biobrick got form igem foundation(BBa_I719005, BBa_K731721, BBa_I14032, BBa_C0012)and parts we constructed(BBa_K1222987, BBa_K1222986, BBa_K1222985). This part is IPTG inducible for regulating the production of GFP antisense.We have designed four sets of GFP antisense to inhibit GFP.(BBa_K1222987, BBa_K1222986, BBa_K1222985)but we have only shown one(GFP antisense 3) of the four antisense as the other three are relatively similar just the GFP antisense is different. The GFP designed is used to test its efficiency on inhibiting(knockdown) the target genes. false false _1536_ 0 18248 9 Not in stock true no. false Tingling Ke component2359962 1 BBa_K1222985 component2359968 1 BBa_I14032 component2359969 1 BBa_K1222003 component2359961 1 BBa_I719005 component2359964 1 BBa_K731721 annotation2359962 1 BBa_K1222985 range2359962 1 32 96 annotation2359968 1 BBa_I14032 range2359968 1 161 197 annotation2359964 1 BBa_K731721 range2359964 1 105 152 annotation2359969 1 BBa_K1222003 range2359969 1 206 1297 annotation2359961 1 BBa_I719005 range2359961 1 1 23 BBa_K1222003 1 BBa_K1222003 LacI 2013-09-23T11:00:00Z 2015-05-08T01:09:43Z We coded LacI from pET11d. Transcription of the Lac promoter is regulated by Lacl, (type of repressor protein) and then combines with the Lac operator sequence thereby inhibiting the transcription process. false false _1536_ 0 18248 9 Not in stock false no. false Tingling Ke BBa_I719005 1 pT7 T7 Promoter 2007-10-23T11:00:00Z 2015-08-31T04:07:53Z --- Released HQ 2013 Just a T7 Promoter false false _128_ 0 2097 9 In stock true None true Imperial 2007 BBa_K1222985 1 BBa_K1222985 Lac operator+GFP antisense 3/pSB1C3 2013-08-25T11:00:00Z 2015-05-08T01:09:43Z We designed and made it by artificial synthesis. A short sequence that inhibits GFP gene is regulated by lac operator . false false _1536_ 0 18249 9 Not in stock false no false Yu-Chun Huang BBa_I14032 1 P(Lac) IQ promoter P(Lac) IQ 2004-08-03T11:00:00Z 2015-08-31T04:07:37Z Plasmid pMAL-p2X Released HQ 2013 Constitutive Promoter, High Transcription false true _4_ 0 171 7 In stock false true Vikram Vijayan, Allen Hsu, Lawrence Fomundam annotation1028344 1 -10 range1028344 1 26 31 annotation1028342 1 P(Lac) IQ range1028342 1 1 37 annotation1028343 1 -35 range1028343 1 3 8 BBa_K731721 1 BBa_K731721 T7 terminator 2012-08-14T11:00:00Z 2015-05-08T01:13:06Z We amplified it directly from pET21b. Released HQ 2013 This is a short terminator by T7phage that we tested with our new teminators' characterization system. false false _977_ 0 12063 9 In stock true This is a standard sequence, very used in many constructs. As i don't have any design considerations i can say that generally to make screening gels of such short segments is preferable to use TBE1X instead of TAE1X. false Giacomo Giacomelli, Anna Depetris annotation2180632 1 T7 terminator range2180632 1 1 48 BBa_I719005_sequence 1 taatacgactcactatagggaga BBa_K1222001_sequence 1 taatacgactcactatagggagatactagagggaattgtgagcggataacaattcctcgattctattaacaagggtatcaccttcaaacttgactttactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttgtactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga BBa_K1222985_sequence 1 ggaattgtgagcggataacaattcctcgattctattaacaagggtatcaccttcaaacttgactt BBa_I14032_sequence 1 tggtgcaaaacctttcgcggtatggcatgatagcgcc BBa_K731721_sequence 1 ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_K1222003_sequence 1 gtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z