LOCUS       BBa_K1223002            1210 bp    DNA     linear       05-May-2024
DEFINITION  P.A.S.E. 2 cassette
ACCESSION   BBa_K1223002
VERSION     BBa_K1223002.1  
FEATURES             Location/Qualifiers
     misc_feature    5..64
                     /label=TyrS Homology region
     promoter        87..191
                     /label=AraC\Lac promoter
     RBS             71..81
                     /label=RBS
     misc_feature    200..1132
                     /label=KanR
     CDS             200..994
                     /label=KanR CDS
     promoter        1083..1132
                     /label=Neo/Kan promoter
     misc_feature    1140..1192
                     /label=homology region
     misc_feature    47..49
                     /label=stop codon (TAG)
     misc_feature    62..64
                     /label=start codon
     misc_feature    200..202
                     /label=stop codon
     misc_feature    992..994
                     /label=start codon
ORIGIN
        1 tagacgtcac ctgggctacc agcccccgct cttgcaattg tttaatctag ttactgcttg
       61 ccatctagta tttctcctct ttcgctatct gtgtgaaatt gttatccgct cacaattgaa
      121 tctatcataa ttgtgagcgc tcacaattgt aaaggttaga tccgctaatc ttatggataa
      181 aaatgctatg cagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
      241 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      301 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      361 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      421 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
      481 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      541 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
      601 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      661 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      721 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      781 tggccagcca cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt
      841 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
      901 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
      961 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
     1021 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     1081 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg
     1141 catcttttca gggtgcaaga cgatcaatct tccacgcatc attttcacgc tgggatcccg
     1201 ggcccgtcga
//