LOCUS       BBa_K1223007             880 bp    DNA     linear       10-May-2024
DEFINITION  cI translational unit with His-tag
ACCESSION   BBa_K1223007
VERSION     BBa_K1223007.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-33 Hexamer
     misc_feature    2..37
                     /label=AraC Binding Site
     misc_feature    44..57
                     /label=Symmetrical Synthetic Operator
     promoter        58..66
                     /label=-10 Hexamer
     misc_feature    72..102
                     /label=Lac Operon operator Sequence
     promoter        1..105
                     /label=BBa_K354000
     misc_feature    118..121
                     /label=conserved
     RBS             114..125
                     /label=BBa_B0034
     CDS             132..845
                     /label=Lambda cI
     CDS             132..845
                     /label=BBa_K1223004
     misc_feature    1..845
                     /label=BBa_K1223005
     misc_feature    854..877
                     /label=6X His-Tag
     misc_feature    878..880
                     /label=stop codon
     misc_feature    854..880
                     /label=BBa_K1223006
ORIGIN
        1 gcatagcatt tttatccata agattagcgg atctaacctt tacaattgtg agcgctcaca
       61 attatgatag attcaattgt gagcggataa caatttcaca cagattacta gagaaagagg
      121 agaaatacta gatgggcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac
      181 gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg
      241 tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg
      301 cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat
      361 ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt
      421 cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac
      481 ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag
      541 ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct
      601 ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc
      661 caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca
      721 gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca
      781 atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg
      841 gctaatacta gaggtgcacc accaccacca tcacgtgtaa
//