LOCUS BBa_K1223007 880 bp DNA linear 26-Dec-2024 DEFINITION cI translational unit with His-tag ACCESSION BBa_K1223007 VERSION BBa_K1223007.1 FEATURES Location/Qualifiers promoter 38..43 /label=-33 Hexamer misc_feature 2..37 /label=AraC Binding Site misc_feature 44..57 /label=Symmetrical Synthetic Operator promoter 58..66 /label=-10 Hexamer misc_feature 72..102 /label=Lac Operon operator Sequence promoter 1..105 /label=BBa_K354000 misc_feature 118..121 /label=conserved RBS 114..125 /label=BBa_B0034 CDS 132..845 /label=Lambda cI CDS 132..845 /label=BBa_K1223004 misc_feature 1..845 /label=BBa_K1223005 misc_feature 854..877 /label=6X His-Tag misc_feature 878..880 /label=stop codon misc_feature 854..880 /label=BBa_K1223006 ORIGIN 1 gcatagcatt tttatccata agattagcgg atctaacctt tacaattgtg agcgctcaca 61 attatgatag attcaattgt gagcggataa caatttcaca cagattacta gagaaagagg 121 agaaatacta gatgggcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac 181 gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg 241 tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg 301 cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat 361 ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt 421 cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac 481 ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag 541 ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct 601 ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc 661 caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca 721 gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca 781 atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg 841 gctaatacta gaggtgcacc accaccacca tcacgtgtaa //