LOCUS BBa_K1223008 3894 bp DNA linear 01-Jan-2026 DEFINITION pUC-57-P.A.S.E.2 ACCESSION BBa_K1223008 VERSION BBa_K1223008.1 FEATURES Location/Qualifiers misc_feature 425..430 /label=XbaI site misc_feature 430..490 /label=Tyr synthetase genom homology region misc_feature 473..475 /label=TAG promoter 512..617 /label=AraC/Lac promoter (BBa_K354000) CDS 626..1420 /label=Neo/KanR CDS RBS 497..508 /label=RBS misc_feature 1566..1618 /label=genome homology region misc_feature 1619..1624 /label=BamHI site misc_feature 2074..2075 /label=Rep. origin CDS 2834..3694 /label=b-lactamase CDS (ampR) ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa 421 tgcatctaga cgtcacctgg gctaccagcc cccgctcttg caattgttta atctagttac 481 tgcttgccat ctagtatttc tcctctttcg ctatctgtgt gaaattgtta tccgctcaca 541 attgaatcta tcataattgt gagcgctcac aattgtaaag gttagatccg ctaatcttat 601 ggataaaaat gctatgcagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga 661 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc 721 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca 781 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg 841 gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga 901 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat 961 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt 1021 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg 1081 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca 1141 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc 1201 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg 1261 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg 1321 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag 1381 cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg 1441 tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc 1501 agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt 1561 cgcttgcatc ttttcagggt gcaagacgat caatcttcca cgcatcattt tcacgctggg 1621 atcccgggcc cgtcgactgc agaggcctgc atgcaagctt ggcgtaatca tggtcatagc 1681 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 1741 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 1801 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 1861 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 1921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 1981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 2041 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 2101 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 2161 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 2221 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 2281 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 2341 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 2401 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 2461 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 2521 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 2581 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 2641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 2701 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 2761 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 2821 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 2881 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 2941 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 3001 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 3061 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 3121 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 3181 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 3241 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 3301 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 3361 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 3421 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 3481 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 3541 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 3601 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 3661 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 3721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 3781 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 3841 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc //