LOCUS       BBa_K1223009            2696 bp    DNA     linear       06-May-2024
DEFINITION  pUC57 backbone (REVERSED)
ACCESSION   BBa_K1223009
VERSION     BBa_K1223009.1  
FEATURES             Location/Qualifiers
     CDS             626..1486
                     /label=b-lactamase CDS (ampR)
     misc_feature    2246..2247
                     /label=Rep. origin
ORIGIN
        1 atgcattcgc gaggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg
       61 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      121 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      181 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
      241 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
      301 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      361 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
      421 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      481 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      541 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      601 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      661 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      721 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      781 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      841 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
      901 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
      961 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1021 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1081 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1141 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1201 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1261 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1321 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1381 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1441 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1501 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1561 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1621 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1681 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1741 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1801 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1861 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1921 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1981 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2041 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2101 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2161 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2221 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2281 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2341 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2401 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2461 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2521 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2581 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2641 ctatgaccat gattacgcca agcttgcatg caggcctctg cagtcgacgg gcccgg
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