LOCUS BBa_K1223010 4850 bp DNA linear 15-Nov-2024 DEFINITION pUC-57-P.A.S.E.1 ACCESSION BBa_K1223010 VERSION BBa_K1223010.1 FEATURES Location/Qualifiers misc_feature 19..73 /label=upstream homology region CDS 234..1049 /label=KanR CDS promoter 1059..1107 /label=cI regulated promoter misc_feature 1059..2088 /label=P.A.S.E 1 death operaton CDS 1134..1610 /label=Lambda lyzosime CDS 1631..1948 /label=Lambda holin CDS misc_feature 2094..2148 /label=downstream homology region misc_feature 1..2148 /label=BBa_K1223001 CDS 2780..3640 /label=b-lactamase CDS (ampR) misc_feature 4400..4401 /label=Rep. origin misc_feature 2155..4850 /label=BBa_K1223009 ORIGIN 1 tcgacgggcc cgggatccgc gtggcccggc gatcttatat tgatcgccta aagtcatcgc 61 tacactacca ctacgggaca cctgactgat ccttcaactc agcaaaagtt cgatttattc 121 aacaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 181 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc 241 atattcaacg ggaaacgtct tgctcccgtc cgcgcttaaa ctccaacatg gacgctgatt 301 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct 361 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca 421 atgatgttac agatgagatg gtccgtctca actggctgac ggagtttatg cctctcccga 481 ccatcaagca ttttatccgt actcctgatg atgcgtggtt actcaccacc gcgattcctg 541 ggaaaacagc cttccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg 601 cgctggccgt gttcctgcgc cggttacatt cgattcctgt ttgtaattgt ccttttaaca 661 gcgatcgtgt atttcgtctt gctcaggcgc aatcacgcat gaataacggt ttggttgatg 721 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc 781 acaagctctt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata 841 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacgg gtcggaatcg 901 cagaccgtta ccaggacctt gccattcttt ggaactgcct cggtgagttt tctccttcat 961 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt 1021 ttcatttgat gctcgatgag tttttctaat aaacctgcta acaccgtgcg tgttgactat 1081 tttacctctg gcggtgataa tggttgcata aacctccaca gtagaagatg gtagaaatca 1141 ataatcaacg taaggcgttc ctcgatatgc tggcgtggtc ggagggaact gataacggac 1201 gtcagaaaac cagaaatcat ggttatgacg tcattgtagg cggagagcta ttcactgatt 1261 actccgatca ccctcgcaaa cttgtcacgc taaacccaaa actcaaatca acaggcgccg 1321 gacgctacca gcttctttcc cgttggtggg atgcctaccg caagcagctt ggcctgaaag 1381 acttctctcc gaaaagtcag gacgctgtgg cattgcagca gattaaggag cgtggcgctt 1441 tacctatgat tgatcgtggt gatatccgtc aggcaatcga ccgttgcagc aatatctggg 1501 cttcactgcc gggcgctggt tatggtcagt tcgagcataa ggctgacagc ctgattgcaa 1561 aattcaaaga agcgggcgga acggtcagag agattgatgt atgaggatct attgggggta 1621 agacctgaag atgccagaaa aacatgacct gttggccgcc attctcgcgg caaaggaaca 1681 aggcatcggg gcaatccttg cgtttgcaat ggcgtacctt cgcggcagat ataatggcgg 1741 tgcgtttaca aaaacagtaa tcgacgcaac gatgtgcgcc attatcgcct ggttcattcg 1801 tgaccttctc gacttcgccg gactaagtag caatctcgct tatataacga gcgtgtttat 1861 cggctacatc ggtactgact cgattggttc gcttatcaaa cgcttcgctg ctaaaaaagc 1921 cggagtagaa gatggtagaa atcaataagg atctgatctc caggcatcaa ataaaacgaa 1981 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2041 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatagg gatcgcacca 2101 acggcgacac gctgaaggcc ttcgaaatga atatctttca ctacgaacta ctagatgcat 2161 tcgcgaggta ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa 2221 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 2281 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 2341 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg 2401 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac 2461 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt 2521 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag 2581 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc 2641 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt 2701 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 2761 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 2821 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 2881 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 2941 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 3001 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca 3061 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 3121 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 3181 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 3241 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 3301 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 3361 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 3421 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 3481 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 3541 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 3601 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 3661 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 3721 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 3781 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 3841 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 3901 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 3961 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 4021 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 4081 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 4141 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 4201 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 4261 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 4321 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 4381 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 4441 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 4501 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 4561 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 4621 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 4681 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 4741 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 4801 ccatgattac gccaagcttg catgcaggcc tctgcagtcg acgggcccgg //