LOCUS BBa_K1223012 5220 bp DNA linear 17-Dec-2024 DEFINITION pKD46 functional unit ACCESSION BBa_K1223012 VERSION BBa_K1223012.1 FEATURES Location/Qualifiers CDS 3..881 /label=AraC CDS CDS 1236..1652 /label=gamma protein CDS CDS 1658..2443 /label=beta protein CDS CDS 2440..3120 /label=exonuclease CDS CDS 3488..4438 /label=RepA101 protein CDS misc_feature 4442..5182 /label=OriR101 replication origin ORIGIN 1 tattatgaca acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg 61 ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa 121 ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc 181 tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa 241 agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa 301 ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg attatccatc 361 ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga 421 tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca 481 aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc cgtattggca 541 aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac 601 tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc tcctggcggg 661 aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt caccaccccc 721 tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa 781 aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac 841 gagtatcccg gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca 901 ttcagagaag aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac 961 tggctcttct cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc 1021 gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc 1081 cacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga 1141 ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt 1201 ttttgggaat tcgagctcta aggaggttat aaaaaatgga tattaatact gaaactgaga 1261 tcaagcaaaa gcattcacta accccctttc ctgttttcct aatcagcccg gcatttcgcg 1321 ggcgatattt tcacagctat ttcaggagtt cagccatgaa cgcttattac attcaggatc 1381 gtcttgaggc tcagagctgg gcgcgtcact accagcagct cgcccgtgaa gagaaagagg 1441 cagaactggc agacgacatg gaaaaaggcc tgccccagca cctgtttgaa tcgctatgca 1501 tcgatcattt gcaacgccac ggggccagca aaaaatccat tacccgtgcg tttgatgacg 1561 atgttgagtt tcaggagcgc atggcagaac acatccggta catggttgaa accattgctc 1621 accaccaggt tgatattgat tcagaggtat aaaacgaatg agtactgcac tcgcaacgct 1681 ggctgggaag ctggctgaac gtgtcggcat ggattctgtc gacccacagg aactgatcac 1741 cactcttcgc cagacggcat ttaaaggtga tgccagcgat gcgcagttca tcgcattact 1801 gatcgttgcc aaccagtacg gccttaatcc gtggacgaaa gaaatttacg cctttcctga 1861 taagcagaat ggcatcgttc cggtggtggg cgttgatggc tggtcccgca tcatcaatga 1921 aaaccagcag tttgatggca tggactttga gcaggacaat gaatcctgta catgccggat 1981 ttaccgcaag gaccgtaatc atccgatctg cgttaccgaa tggatggatg aatgccgccg 2041 cgaaccattc aaaactcgcg aaggcagaga aatcacgggg ccgtggcagt cgcatcccaa 2101 acggatgtta cgtcataaag ccatgattca gtgtgcccgt ctggccttcg gatttgctgg 2161 tatctatgac aaggatgaag ccgagcgcat tgtcgaaaat actgcataca ctgcagaacg 2221 tcagccggaa cgcgacatca ctccggttaa cgatgaaacc atgcaggaga ttaacactct 2281 gctgatcgcc ctggataaaa catgggatga cgacttattg ccgctctgtt cccagatatt 2341 tcgccgcgac attcgtgcat cgtcagaact gacacaggcc gaagcagtaa aagctcttgg 2401 attcctgaaa cagaaagccg cagagcagaa ggtggcagca tgacaccgga cattatcctg 2461 cagcgtaccg ggatcgatgt gagagctgtc gaacaggggg atgatgcgtg gcacaaatta 2521 cggctcggcg tcatcaccgc ttcagaagtt cacaacgtga tagcaaaacc ccgctccgga 2581 aagaagtggc ctgacatgaa aatgtcctac ttccacaccc tgcttgctga ggtttgcacc 2641 ggtgtggctc cggaagttaa cgctaaagca ctggcctggg gaaaacagta cgagaacgac 2701 gccagaaccc tgtttgaatt cacttccggc gtgaatgtta ctgaatcccc gatcatctat 2761 cgcgacgaaa gtatgcgtac cgcctgctct cccgatggtt tatgcagtga cggcaacggc 2821 cttgaactga aatgcccgtt tacctcccgg gatttcatga agttccggct cggtggtttc 2881 gaggccataa agtcagctta catggcccag gtgcagtaca gcatgtgggt gacgcgaaaa 2941 aatgcctggt actttgccaa ctatgacccg cgtatgaagc gtgaaggcct gcattatgtc 3001 gtgattgagc gggatgaaaa gtacatggcg agttttgacg agatcgtgcc ggagttcatc 3061 gaaaaaatgg acgaggcact ggctgaaatt ggttttgtat ttggggagca atggcgatga 3121 cgcatcctca cgataatatc cgggtaggcg caatcacttt cgtctactcc gttacaaagc 3181 gaggctgggt atttcccggc ctttctgtta tccgaaatcc actgaaagca cagcggctgg 3241 ctgaggagat aaataataaa cgaggggctg tatgcacaaa gcatcttctg ttgagttaag 3301 aacgagtatc gagatggcac atagccttgc tcaaattgga atcaggtttg tgccaatacc 3361 agtagaaaca gacgaagaat ccatgggtat ggacagtttt ccctttgata tgtaacggtg 3421 aacagttgtt ctacttttgt ttgttagtct tgatgcttca ctgatagata caagagccat 3481 aagaacctca gatccttccg tatttagcca gtatgttctc tagtgtggtt cgttgttttt 3541 gcgtgagcca tgagaacgaa ccattgagat catacttact ttgcatgtca ctcaaaaatt 3601 ttgcctcaaa actggtgagc tgaatttttg cagttaaagc atcgtgtagt gtttttctta 3661 gtccgttacg taggtaggaa tctgatgtaa tggttgttgg tattttgtca ccattcattt 3721 ttatctggtt gttctcaagt tcggttacga gatccatttg tctatctagt tcaacttgga 3781 aaatcaacgt atcagtcggg cggcctcgct tatcaaccac caatttcata ttgctgtaag 3841 tgtttaaatc tttacttatt ggtttcaaaa cccattggtt aagcctttta aactcatggt 3901 agttattttc aagcattaac atgaacttaa attcatcaag gctaatctct atatttgcct 3961 tgtgagtttt cttttgtgtt agttctttta ataaccactc ataaatcctc atagagtatt 4021 tgttttcaaa agacttaaca tgttccagat tatattttat gaattttttt aactggaaaa 4081 gataaggcaa tatctcttca ctaaaaacta attctaattt ttcgcttgag aacttggcat 4141 agtttgtcca ctggaaaatc tcaaagcctt taaccaaagg attcctgatt tccacagttc 4201 tcgtcatcag ctctctggtt gctttagcta atacaccata agcattttcc ctactgatgt 4261 tcatcatctg agcgtattgg ttataagtga acgataccgt ccgttctttc cttgtagggt 4321 tttcaatcgt ggggttgagt agtgccacac agcataaaat tagcttggtt tcatgctccg 4381 ttaagtcata gcgactaatc gctagttcat ttgctttgaa aacaactaat tcagacatac 4441 atctcaattg gtctaggtga ttttaatcac tataccaatt gagatgggct agtcaatgat 4501 aattactagt ccttttcctt tgagttgtgg gtatctgtaa attctgctag acctttgctg 4561 gaaaacttgt aaattctgct agaccctctg taaattccgc tagacctttg tgtgtttttt 4621 ttgtttatat tcaagtggtt ataatttata gaataaagaa agaataaaaa aagataaaaa 4681 gaatagatcc cagccctgtg tataactcac tactttagtc agttccgcag tattacaaaa 4741 ggatgtcgca aacgctgttt gctcctctac aaaacagacc ttaaaaccct aaaggcttaa 4801 gtagcaccct cgcaagctcg gttgcggccg caatcgggca aatcgctgaa tattcctttt 4861 gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt tcgctgcgct 4921 cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat ggattcatgc 4981 aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc gttttatggc 5041 gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc cctctgattt 5101 tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct aatgcaccca 5161 gtaaggcagc ggtatcatca acggggtctg acgctcagtg gaacgaaaac tcacgttaag //