LOCUS       BBa_K1230002            1035 bp    DNA     linear       26-Dec-2024
DEFINITION  YFP generator with LsrA promoter, with terminator as a prefix
ACCESSION   BBa_K1230002
VERSION     BBa_K1230002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..39
                     /label=B1006
     stem_loop       10..31
                     /label=modified thr terminator
     polyA_site      32..39
                     /label=PolyA
     polyA_site      1..9
                     /label=PolyA
     terminator      1..39
                     /label=BBa_B1006
     RBS             159..170
                     /label=RBS BBa_B0034
     promoter        48..149
                     /label=BBa_K117002
     misc_feature    162..165
                     /label=conserved
     RBS             158..169
                     /label=BBa_B0034
     CDS             176..898
                     /label=BBa_E0030
     stem_loop       918..961
                     /label=stem_loop
     misc_feature    907..986
                     /label=BBa_B0010
     terminator      907..986
                     /label=BBa_B0010
     stem_loop       1002..1021
                     /label=T7 TE
     misc_feature    1028..1028
                     /label=stop
     polyA_site      1022..1035
                     /label=polya
     misc_feature    995..1035
                     /label=BBa_B0012
     terminator      995..1035
                     /label=BBa_B0012
     misc_feature    48..1035
                     /label=BBa_K117008
ORIGIN
        1 aaaaaaaaac cccgcccctg acagggcggg gttttttttt actagagcac aacatcaact
       61 gagtgcatgc cttatttaat catgtaacta ttcttacgac ttcaaacgtc aaacagtctt
      121 aacacttatt taattaaaaa gaggagaaat actagagaaa gaggagaaat actagatggt
      181 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      241 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      301 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      361 gaccaccttc ggctacggcc tgcaatgctt cgcccgctac cccgaccaca tgaagctgca
      421 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      481 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      541 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      601 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
      661 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
      721 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
      781 gagctaccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
      841 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaataata
      901 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      961 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1021 ctttctgcgt ttata
//