LOCUS       BBa_K1230010            2244 bp    DNA     linear       09-Jun-2024
DEFINITION  araC-pBad-rbs-LL37-rbs-GFP
ACCESSION   BBa_K1230010
VERSION     BBa_K1230010.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             21..899
                     /label=AraC
     misc_feature    929..944
                     /label=AraO
     misc_feature    1086..1097
                     /label=AraO1
     promoter        1173..1201
                     /label=AraPromoter
     promoter        21..1229
                     /label=BBa_K808000
     misc_feature    1..1229
                     /label=BBa_K1230009
     misc_feature    1236..1238
                     /label=start
     CDS             1236..1358
                     /label=BBa_K875009
     RBS             1367..1381
                     /label=RBS-1\Strong
     RBS             1374..1378
                     /label=RBS
     misc_feature    1367..1381
                     /label=BBa_B0030
     RBS             1367..1381
                     /label=BBa_B0030
     CDS             1388..2107
                     /label=GFP protein
     CDS             1388..2107
                     /label=BBa_E0040
     stem_loop       2127..2170
                     /label=stem_loop
     misc_feature    2116..2195
                     /label=BBa_B0010
     terminator      2116..2195
                     /label=BBa_B0010
     stem_loop       2211..2230
                     /label=T7 TE
     misc_feature    2237..2237
                     /label=stop
     polyA_site      2231..2244
                     /label=polya
     misc_feature    2204..2244
                     /label=BBa_B0012
     terminator      2204..2244
                     /label=BBa_B0012
     misc_feature    1367..2244
                     /label=BBa_E0840
     misc_feature    1236..2244
                     /label=BBa_K1230003
ORIGIN
        1 aaagaggaga aatactagag ttatgacaac ttgacggcta catcattcac tttttcttca
       61 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa
      121 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg
      181 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc
      241 cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg
      301 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg
      361 cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt
      421 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg
      481 gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga
      541 aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc
      601 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga
      661 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc
      721 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc
      781 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc
      841 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata
      901 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc
      961 cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa
     1021 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat
     1081 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata
     1141 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact
     1201 gtttctccat acccgttttt tgggctaact actagatgct gctgggcgat ttttttcgca
     1261 aaagcaaaga aaaaattggc aaagaattta aacgcattgt gcagcgcatt aaagattttc
     1321 tgcgcaacct ggtgccgcgc accgaaagct aataataata ctagagatta aagaggagaa
     1381 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     1441 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     1501 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1561 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
     1621 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     1681 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     1741 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     1801 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     1861 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1921 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1981 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     2041 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     2101 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2161 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2221 cgggtgggcc tttctgcgtt tata
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