##gff-version 3
##sequence-region BBa_K1230014 1 1505
BBa_K1230014	.	engineered_region	887	1505	.	+	0	ID=BBa_K1230004;Name=BBa_K1230004
BBa_K1230014	.	CDS	1113	1505	.	+	0	ID=BBa_K1230000;Name=BBa_K1230000;Parent=BBa_K1230004
BBa_K1230014	.	point_mutation	1244	1244	.	+	0	ID=annotation2335368;Name=G to C mutation;Parent=BBa_K1230000
BBa_K1230014	.	start_codon	1113	1115	.	+	0	ID=annotation2335367;Name=start;Parent=BBa_K1230000
BBa_K1230014	.	stop_codon	1500	1505	.	+	0	ID=annotation2335366;Name=stop;Parent=BBa_K1230000
BBa_K1230014	.	CDS	1113	1505	.	+	0	ID=annotation2335365;Name=MarA;Parent=BBa_K1230000
BBa_K1230014	.	engineered_region	887	1106	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1230004
BBa_K1230014	.	promoter	887	1086	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1230014	.	promoter	1047	1052	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1230014	.	promoter	1023	1028	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1230014	.	start_codon	1059	1059	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1230014	.	non_covalent_binding_site	1052	1086	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1230014	.	CDS	887	974	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1230014	.	non_covalent_binding_site	975	1012	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1230014	.	engineered_region	887	1086	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1230014	.	ribosome_entry_site	1095	1106	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1230014	.	conserved_region	1099	1102	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1230014	.	engineered_region	1	878	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1230014	.	CDS	22	741	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1230014	.	CDS	22	741	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1230014	.	terminator	750	829	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1230014	.	engineered_region	750	829	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1230014	.	stem_loop	761	804	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1230014	.	terminator	838	878	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1230014	.	stem_loop	845	864	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1230014	.	stop_codon	871	871	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1230014	.	engineered_region	838	878	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1230014	.	polyA_site	865	878	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1230014	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1230014	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1230014	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1230014	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
>BBa_K1230014
attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatg
gtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatt
tatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcg
agatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatat
ttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagtt
aaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatac
atcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaac
tagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacaca
atctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattaca
catggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg
tttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttac
actttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
tactagatgacgatgtccagacgcaatactgacgctattaccattcatagcattttggactggatcgaggacaacctgg
aatcgccactgtcactggagaaagtgtcagagcgttcgggttactccaaatggcacctccaacggatgtttaaaaaaga
aaccggtcattcattaggccaatacatccgcagccgtaagatgacggaaatcgcgcaaaagctgaaggaaagtaacgag
ccgatactctatctggcagaacgatatggcttcgagtcgcaacaaactctgacccgaaccttcaaaaattactttgatg
ttccgccgcataaataccggatgaccaatatgcagggcgaatcgcgctttttacatccattaaatcattacaacagcta
ataa