LOCUS BBa_K1230015 2868 bp DNA linear 22-Dec-2024 DEFINITION araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator ACCESSION BBa_K1230015 VERSION BBa_K1230015.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 21..899 /label=AraC misc_feature 929..944 /label=AraO misc_feature 1086..1097 /label=AraO1 promoter 1173..1201 /label=AraPromoter promoter 21..1229 /label=BBa_K808000 misc_feature 1..1229 /label=BBa_K1230009 CDS 1236..1355 /label=LL-37 misc_feature 1236..1238 /label=Start misc_feature 1349..1355 /label=Stop misc_feature 1236..1355 /label=BBa_K1230001 misc_feature 1..1355 /label=BBa_K1230011 RBS 1364..1378 /label=RBS-1\Strong RBS 1371..1375 /label=RBS misc_feature 1364..1378 /label=BBa_B0030 RBS 1364..1378 /label=BBa_B0030 CDS 1385..2104 /label=GFP protein CDS 1385..2104 /label=BBa_E0040 stem_loop 2124..2167 /label=stem_loop misc_feature 2113..2192 /label=BBa_B0010 terminator 2113..2192 /label=BBa_B0010 stem_loop 2208..2227 /label=T7 TE misc_feature 2234..2234 /label=stop polyA_site 2228..2241 /label=polya misc_feature 2201..2241 /label=BBa_B0012 terminator 2201..2241 /label=BBa_B0012 misc_feature 1364..2241 /label=BBa_E0840 CDS 2250..2337 /label=end of LacI coding region (inactive) misc_feature 2250..2449 /label=BBa_R0010 misc_feature 2338..2375 /label=CAP binding site promoter 2386..2391 /label=-35 promoter 2410..2415 /label=-10 misc_feature 2415..2449 /label=LacI binding site misc_feature 2422..2422 /label=start promoter 2250..2449 /label=BBa_R0010 misc_feature 2462..2465 /label=conserved RBS 2458..2469 /label=BBa_B0034 misc_feature 2250..2469 /label=BBa_J04500 CDS 2476..2868 /label=MarA misc_feature 2863..2868 /label=stop misc_feature 2476..2478 /label=start misc_feature 2607..2607 /label=G to C mutation CDS 2476..2868 /label=BBa_K1230000 misc_feature 2250..2868 /label=BBa_K1230004 misc_feature 1364..2868 /label=BBa_K1230014 ORIGIN 1 aaagaggaga aatactagag ttatgacaac ttgacggcta catcattcac tttttcttca 61 caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa 121 tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg 181 ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc 241 cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg 301 acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg 361 cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt 421 aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg 481 gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga 541 aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc 601 ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga 661 tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc 721 tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc 781 agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc 841 accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata 901 cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc 961 cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa 1021 agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat 1081 aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata 1141 gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca actctctact 1201 gtttctccat acccgttttt tgggctaact actagatgct gctgggtgat ttcttccgga 1261 aatctaaaga gaagattggc aaagagttta aaagaattgt ccagagaatc aaggattttt 1321 tgcggaatct tgtacccagg acagagtcct aataatacta gagattaaag aggagaaata 1381 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga 1441 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata 1501 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac 1561 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa 1621 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt 1681 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct 1741 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca 1801 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa 1861 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc 1921 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca 1981 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt 2041 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata 2101 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 2161 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 2221 gtgggccttt ctgcgtttat atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg 2281 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 2341 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct 2401 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa 2461 gaggagaaat actagatgac gatgtccaga cgcaatactg acgctattac cattcatagc 2521 attttggact ggatcgagga caacctggaa tcgccactgt cactggagaa agtgtcagag 2581 cgttcgggtt actccaaatg gcacctccaa cggatgttta aaaaagaaac cggtcattca 2641 ttaggccaat acatccgcag ccgtaagatg acggaaatcg cgcaaaagct gaaggaaagt 2701 aacgagccga tactctatct ggcagaacga tatggcttcg agtcgcaaca aactctgacc 2761 cgaaccttca aaaattactt tgatgttccg ccgcataaat accggatgac caatatgcag 2821 ggcgaatcgc gctttttaca tccattaaat cattacaaca gctaataa //