##gff-version 3
##sequence-region BBa_K1230015 1 2868
BBa_K1230015	.	engineered_region	1364	2868	.	+	0	ID=BBa_K1230014;Name=BBa_K1230014
BBa_K1230015	.	engineered_region	2250	2868	.	+	0	ID=BBa_K1230004;Name=BBa_K1230004;Parent=BBa_K1230014
BBa_K1230015	.	engineered_region	2250	2469	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1230004
BBa_K1230015	.	promoter	2250	2449	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1230015	.	non_covalent_binding_site	2338	2375	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1230015	.	start_codon	2422	2422	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1230015	.	promoter	2386	2391	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1230015	.	CDS	2250	2337	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1230015	.	engineered_region	2250	2449	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1230015	.	promoter	2410	2415	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1230015	.	non_covalent_binding_site	2415	2449	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1230015	.	ribosome_entry_site	2458	2469	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1230015	.	conserved_region	2462	2465	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1230015	.	CDS	2476	2868	.	+	0	ID=BBa_K1230000;Name=BBa_K1230000;Parent=BBa_K1230004
BBa_K1230015	.	CDS	2476	2868	.	+	0	ID=annotation2335365;Name=MarA;Parent=BBa_K1230000
BBa_K1230015	.	point_mutation	2607	2607	.	+	0	ID=annotation2335368;Name=G to C mutation;Parent=BBa_K1230000
BBa_K1230015	.	start_codon	2476	2478	.	+	0	ID=annotation2335367;Name=start;Parent=BBa_K1230000
BBa_K1230015	.	stop_codon	2863	2868	.	+	0	ID=annotation2335366;Name=stop;Parent=BBa_K1230000
BBa_K1230015	.	engineered_region	1364	2241	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K1230014
BBa_K1230015	.	ribosome_entry_site	1364	1378	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1230015	.	ribosome_entry_site	1371	1375	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1230015	.	ribosome_entry_site	1364	1378	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1230015	.	engineered_region	1364	1378	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1230015	.	terminator	2113	2192	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1230015	.	engineered_region	2113	2192	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1230015	.	stem_loop	2124	2167	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1230015	.	CDS	1385	2104	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1230015	.	CDS	1385	2104	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1230015	.	terminator	2201	2241	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1230015	.	engineered_region	2201	2241	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1230015	.	stop_codon	2234	2234	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1230015	.	polyA_site	2228	2241	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1230015	.	stem_loop	2208	2227	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1230015	.	engineered_region	1	1355	.	+	0	ID=BBa_K1230011;Name=BBa_K1230011
BBa_K1230015	.	engineered_region	1	1229	.	+	0	ID=BBa_K1230009;Name=BBa_K1230009;Parent=BBa_K1230011
BBa_K1230015	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K1230009
BBa_K1230015	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1230015	.	promoter	21	1229	.	+	0	ID=BBa_K808000;Name=BBa_K808000;Parent=BBa_K1230009
BBa_K1230015	.	non_covalent_binding_site	929	944	.	+	0	ID=annotation2200758;Name=AraO;Parent=BBa_K808000
BBa_K1230015	.	promoter	1173	1201	.	+	0	ID=annotation2200760;Name=AraPromoter;Parent=BBa_K808000
BBa_K1230015	.	CDS	21	899	.	+	0	ID=annotation2200757;Name=AraC;Parent=BBa_K808000
BBa_K1230015	.	non_covalent_binding_site	1086	1097	.	+	0	ID=annotation2200759;Name=AraO1;Parent=BBa_K808000
BBa_K1230015	.	polypeptide_domain	1236	1355	.	+	0	ID=BBa_K1230001;Name=BBa_K1230001;Parent=BBa_K1230011
BBa_K1230015	.	start_codon	1236	1238	.	+	0	ID=annotation2335370;Name=Start;Parent=BBa_K1230001
BBa_K1230015	.	stop_codon	1349	1355	.	+	0	ID=annotation2335371;Name=Stop;Parent=BBa_K1230001
BBa_K1230015	.	CDS	1236	1355	.	+	0	ID=annotation2335369;Name=LL-37;Parent=BBa_K1230001
>BBa_K1230015
aaagaggagaaatactagagttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgc
tcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggt
ggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgcta
atccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaa
aattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactc
gttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttcccct
tgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtat
tggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattc
gcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaat
tctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggt
cgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccgg
cagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgc
atcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattc
tgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattga
ttatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgcttttta
tcgcaactctctactgtttctccatacccgttttttgggctaactactagatgctgctgggtgatttcttccggaaatc
taaagagaagattggcaaagagtttaaaagaattgtccagagaatcaaggattttttgcggaatcttgtacccaggaca
gagtcctaataatactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccc
aattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatac
ggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtt
atggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtta
tgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacc
cttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaact
ataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacat
tgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagac
aaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttg
taacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcatt
aatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactca
ttaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
tactagagaaagaggagaaatactagatgacgatgtccagacgcaatactgacgctattaccattcatagcattttgga
ctggatcgaggacaacctggaatcgccactgtcactggagaaagtgtcagagcgttcgggttactccaaatggcacctc
caacggatgtttaaaaaagaaaccggtcattcattaggccaatacatccgcagccgtaagatgacggaaatcgcgcaaa
agctgaaggaaagtaacgagccgatactctatctggcagaacgatatggcttcgagtcgcaacaaactctgacccgaac
cttcaaaaattactttgatgttccgccgcataaataccggatgaccaatatgcagggcgaatcgcgctttttacatcca
ttaaatcattacaacagctaataa