##gff-version 3
##sequence-region BBa_K1230016 1 1741
BBa_K1230016	.	engineered_region	1	619	.	+	0	ID=BBa_K1230004;Name=BBa_K1230004
BBa_K1230016	.	CDS	227	619	.	+	0	ID=BBa_K1230000;Name=BBa_K1230000;Parent=BBa_K1230004
BBa_K1230016	.	start_codon	227	229	.	+	0	ID=annotation2335367;Name=start;Parent=BBa_K1230000
BBa_K1230016	.	stop_codon	614	619	.	+	0	ID=annotation2335366;Name=stop;Parent=BBa_K1230000
BBa_K1230016	.	CDS	227	619	.	+	0	ID=annotation2335365;Name=MarA;Parent=BBa_K1230000
BBa_K1230016	.	point_mutation	358	358	.	+	0	ID=annotation2335368;Name=G to C mutation;Parent=BBa_K1230000
BBa_K1230016	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500;Parent=BBa_K1230004
BBa_K1230016	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_K1230016	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K1230016	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K1230016	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K1230016	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K1230016	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K1230016	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K1230016	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K1230016	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_K1230016	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1230016	.	engineered_region	628	1741	.	+	0	ID=BBa_K1230012;Name=BBa_K1230012
BBa_K1230016	.	polypeptide_domain	1622	1741	.	+	0	ID=BBa_K1230001;Name=BBa_K1230001;Parent=BBa_K1230012
BBa_K1230016	.	start_codon	1622	1624	.	+	0	ID=annotation2335370;Name=Start;Parent=BBa_K1230001
BBa_K1230016	.	stop_codon	1735	1741	.	+	0	ID=annotation2335371;Name=Stop;Parent=BBa_K1230001
BBa_K1230016	.	CDS	1622	1741	.	+	0	ID=annotation2335369;Name=LL-37;Parent=BBa_K1230001
BBa_K1230016	.	engineered_region	628	1615	.	+	0	ID=BBa_K1230008;Name=BBa_K1230008;Parent=BBa_K1230012
BBa_K1230016	.	promoter	1514	1615	.	+	0	ID=BBa_K117002;Name=BBa_K117002;Parent=BBa_K1230008
BBa_K1230016	.	ribosome_entry_site	1625	1636	.	+	0	ID=annotation1979372;Name=RBS BBa_B0034;Parent=BBa_K117002
BBa_K1230016	.	engineered_region	628	1505	.	+	0	ID=BBa_E0840;Name=BBa_E0840;Parent=BBa_K1230008
BBa_K1230016	.	CDS	649	1368	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1230016	.	CDS	649	1368	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1230016	.	terminator	1465	1505	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1230016	.	engineered_region	1465	1505	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1230016	.	stem_loop	1472	1491	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1230016	.	stop_codon	1498	1498	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1230016	.	polyA_site	1492	1505	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1230016	.	terminator	1377	1456	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1230016	.	engineered_region	1377	1456	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1230016	.	stem_loop	1388	1431	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1230016	.	ribosome_entry_site	628	642	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1230016	.	ribosome_entry_site	635	639	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1230016	.	engineered_region	628	642	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1230016	.	ribosome_entry_site	628	642	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_K1230016
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgacgatgtc
cagacgcaatactgacgctattaccattcatagcattttggactggatcgaggacaacctggaatcgccactgtcactg
gagaaagtgtcagagcgttcgggttactccaaatggcacctccaacggatgtttaaaaaagaaaccggtcattcattag
gccaatacatccgcagccgtaagatgacggaaatcgcgcaaaagctgaaggaaagtaacgagccgatactctatctggc
agaacgatatggcttcgagtcgcaacaaactctgacccgaaccttcaaaaattactttgatgttccgccgcataaatac
cggatgaccaatatgcagggcgaatcgcgctttttacatccattaaatcattacaacagctaataatactagagattaa
agaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgat
gttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttattt
gcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagata
cccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttc
aaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaag
gtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcat
ggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagca
gaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctg
ccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatgg
catggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagcacaacatcaactgagtgcatgccttatttaatcatgtaactattcttacgacttcaaacgtcaaac
agtcttaacacttatttaattaaaaagaggagaaatactagatgctgctgggtgatttcttccggaaatctaaagagaa
gattggcaaagagtttaaaagaattgtccagagaatcaaggattttttgcggaatcttgtacccaggacagagtcctaa
taa