BBa_K123002
1
BBa_K123002
LacIQ ERE TetR
2008-10-27T12:00:00Z
2015-05-08T01:09:44Z
The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element
This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase.
false
false
_241_
0
2742
9
It's complicated
true
There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.
true
Jason Gardiner
annotation1992723
1
BBa_C0040
range1992723
1
44
703
annotation1992722
1
SsrA
range1992722
1
664
697
annotation1992718
1
-35
range1992718
1
3
8
annotation1992733
1
ERE
range1992733
1
43
57
annotation1992724
1
barcode
range1992724
1
704
728
annotation1992721
1
tetR
range1992721
1
47
663
annotation1992719
1
-10
range1992719
1
26
31
annotation1992720
1
BBa_I14032
range1992720
1
1
37
annotation1992717
1
P(Lac) IQ
range1992717
1
1
37
BBa_K123002_sequence
1
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z