LOCUS       BBa_K123009             3266 bp    DNA     linear       26-Nov-2024
DEFINITION  lacIq->BisdB
ACCESSION   BBa_K123009
VERSION     BBa_K123009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     misc_feature    1..37
                     /label=BBa_I14032
     CDS             47..663
                     /label=tetR
     misc_feature    664..697
                     /label=SsrA
     misc_feature    44..703
                     /label=BBa_C0040
     misc_feature    704..728
                     /label=barcode
     misc_feature    43..57
                     /label=ERE
     promoter        1..742
                     /label=BBa_K123002
     misc_feature    751..769
                     /label=TetR 1
     misc_feature    751..804
                     /label=BBa_R0040
     promoter        770..775
                     /label=-35
     misc_feature    776..794
                     /label=TetR 2
     promoter        793..798
                     /label=-10
     promoter        751..804
                     /label=BBa_R0040
     CDS             811..1485
                     /label=mrfp1
     misc_feature    1492..1516
                     /label=Help:Barcodes
     CDS             811..1516
                     /label=BBa_E1010
     misc_feature    1525..1543
                     /label=TetR 1
     misc_feature    1525..1578
                     /label=BBa_R0040
     promoter        1544..1549
                     /label=-35
     misc_feature    1550..1568
                     /label=TetR 2
     promoter        1567..1572
                     /label=-10
     promoter        1525..1578
                     /label=BBa_R0040
     CDS             1585..1914
                     /label=BBa_K123000
     misc_feature    1923..1941
                     /label=TetR 1
     misc_feature    1923..1976
                     /label=BBa_R0040
     promoter        1942..1947
                     /label=-35
     misc_feature    1948..1966
                     /label=TetR 2
     promoter        1965..1970
                     /label=-10
     promoter        1923..1976
                     /label=BBa_R0040
     CDS             1983..3266
                     /label=BBa_K123001
     misc_feature    1525..3266
                     /label=BBa_K123008
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagaggtcag agtgaccatg
       61 tccagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga
      121 atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg
      181 tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat
      241 aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat
      301 aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta
      361 ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc
      421 caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact
      481 ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct
      541 actactgata gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt
      601 gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt
      661 aaatgtgaaa gtgggtccgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac
      721 tgatagtgct agtgtagatc actactagag tccctatcag tgatagagat tgacatccct
      781 atcagtgata gagatactga gcactactag atggcttcct ccgaagacgt tatcaaagag
      841 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
      901 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
      961 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
     1021 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
     1081 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
     1141 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
     1201 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
     1261 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
     1321 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
     1381 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     1441 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag
     1501 tgctagtgta gatcgctact agagtcccta tcagtgatag agattgacat ccctatcagt
     1561 gatagagata ctgagcacta ctagatggcc ggccctcata tccaagtgac tacccgcgat
     1621 ggggagatac gcgaactcga cgtcgcagcc tccgggttcc tgatggaagc ccttcgcgac
     1681 gccaatatcg acggcgtcga ggcgatgtgc ggcggatgct gctcctgcgc gacctgccac
     1741 gtctacatcg acgctgctcc cgccgggacc ctgccgccgg tctcctccga cgaggagatg
     1801 ctgctttccg gcctggtctc gacccccgga cggtcgcggc tctcctgcca aattccggtc
     1861 acggccgaac tggatggcct taagctcacg atcccgccgg actccaccgg ttaatactag
     1921 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact
     1981 agatggccgg caaccctcag acactgccgg tctttccaga cctcgacatc ttttcgcccg
     2041 aatatgcctg caaccgcgaa aaatacgcag cccgcgccct tagggactat ccgctgcact
     2101 tctataagcc gctgaatttg tggatcgtca gcaagcacaa ggatgtgcgc tcggccttgt
     2161 tcactccgca ggtcttttct tctgtcgcgt ttggcctgct ccccccgccc gatgatatcg
     2221 cgccgcgcgt gccggacctt tacaccgacg tgcatctgcc ctcgatggac ccgccggagc
     2281 acaccaagct cagggttccc gtccagcaag ccctcctccc cggtcggctg gtgggtaagg
     2341 acgaggtcgt tcgccggatc gccaacgaac ttatcgatac cttcatcgac aagggcgaat
     2401 gcgatctcct ccacgatttc agctacaaac ttgcgctcta cttgatcgtc gatctgctcg
     2461 ggatccccaa ggaacgggcg gaagattatc atcgctggtc caactgcttc ttccagctgt
     2521 tcacccctaa ggtaccggag cgcgccgacg caagattctt cgtgccgatg ccggaagagg
     2581 tactgcggca gaactgggaa ggcctggcgg aggcgaacga ctacctgcgc gaagtcgtcg
     2641 agaaccttga tcgcaatccc ggcaacaaca tgctgtcaaa cctgttgcag ctgcgcgaac
     2701 cggacggctc acgcacgatc acgatcagcg ccaatgtttg caacgcactg gaatttgggg
     2761 cggctggtca tgacaccacg gcaacgctga tcgcgcacct cacctatttc gtgctgacca
     2821 cgccggacct caaggacact cttaccgagg atccgtcgct gatccccgcg gcgatttccg
     2881 aaacgctgcg ccgtcgctgt ccggtcgacg ggctgttccg gcgaacgctg tcggatgtag
     2941 aactatgcgg ccagaaaatc gaatccggat cgatcgtcta cctcgatctc accgctgcaa
     3001 atcttgaccc cgatgtattc cccgaacctg agacgtttcg cctgaaccgc gacaatatca
     3061 aggaaatggt ctctttcggc ttcggtcgtc acgtctgcgc aggacagtac ctgtcccgta
     3121 tcgaagcaaa agcggcctac gaagaactga tgcgccgtat tcccaacatg cgtctcgctg
     3181 atggtttcaa gctggaatac atgccttccg tggcgacgac ggttctcaag gggcttccgc
     3241 tggtctggga caaaaacacc ggttaa
//