##gff-version 3
##sequence-region BBa_K123009 1 3266
BBa_K123009	.	CDS	811	1516	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K123009	.	CDS	811	1485	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K123009	.	engineered_tag	1492	1516	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K123009	.	promoter	751	804	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K123009	.	engineered_region	751	804	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K123009	.	promoter	770	775	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K123009	.	non_covalent_binding_site	776	794	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K123009	.	promoter	793	798	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K123009	.	non_covalent_binding_site	751	769	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K123009	.	engineered_region	1525	3266	.	+	0	ID=BBa_K123008;Name=BBa_K123008
BBa_K123009	.	promoter	1923	1976	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K123008
BBa_K123009	.	engineered_region	1923	1976	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K123009	.	promoter	1942	1947	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K123009	.	non_covalent_binding_site	1948	1966	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K123009	.	promoter	1965	1970	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K123009	.	non_covalent_binding_site	1923	1941	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K123009	.	CDS	1983	3266	.	+	0	ID=BBa_K123001;Name=BBa_K123001;Parent=BBa_K123008
BBa_K123009	.	promoter	1525	1578	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K123008
BBa_K123009	.	engineered_region	1525	1578	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K123009	.	promoter	1544	1549	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K123009	.	non_covalent_binding_site	1550	1568	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K123009	.	promoter	1567	1572	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K123009	.	non_covalent_binding_site	1525	1543	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K123009	.	CDS	1585	1914	.	+	0	ID=BBa_K123000;Name=BBa_K123000;Parent=BBa_K123008
BBa_K123009	.	promoter	1	742	.	+	0	ID=BBa_K123002;Name=BBa_K123002
BBa_K123009	.	non_covalent_binding_site	43	57	.	+	0	ID=annotation1992733;Name=ERE;Parent=BBa_K123002
BBa_K123009	.	CDS	47	663	.	+	0	ID=annotation1992721;Name=tetR;Parent=BBa_K123002
BBa_K123009	.	engineered_region	1	37	.	+	0	ID=annotation1992720;Name=BBa_I14032;Parent=BBa_K123002
BBa_K123009	.	engineered_region	704	728	.	+	0	ID=annotation1992724;Name=barcode;Parent=BBa_K123002
BBa_K123009	.	promoter	3	8	.	+	0	ID=annotation1992718;Name=-35;Parent=BBa_K123002
BBa_K123009	.	tag	664	697	.	+	0	ID=annotation1992722;Name=SsrA;Parent=BBa_K123002
BBa_K123009	.	engineered_region	44	703	.	+	0	ID=annotation1992723;Name=BBa_C0040;Parent=BBa_K123002
BBa_K123009	.	promoter	26	31	.	+	0	ID=annotation1992719;Name=-10;Parent=BBa_K123002
BBa_K123009	.	operator	1	37	.	+	0	ID=annotation1992717;Name=P(Lac) IQ;Parent=BBa_K123002
>BBa_K123009
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagta
aagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagct
aggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgtta
gataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagtttta
gatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactct
cgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcat
tttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagta
tgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaatt
gatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagct
taataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgata
gagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaagg
ttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactg
aaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacg
ttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaactt
cgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgt
ggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacc
cggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaa
aaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccac
aacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagt
gctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacta
ctagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttc
ctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgcc
acgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggt
ctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccg
ccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagca
ctactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaac
cgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagca
agcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccga
tgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctc
agggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaac
ttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgt
cgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccct
aaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctgg
cggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgtt
gcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggct
ggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactctta
ccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcg
aacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaat
cttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggct
tcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtat
tcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccg
ctggtctgggacaaaaacaccggttaa