BBa_K123009
type: ComponentDefinition
persistent identity: BBa K123009
display id: BBa_K123009
version: 1
was generated by: iGEM to SBOL conversion
description: lacIq->BisdB
created: 2008-10-28T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Bisphenolics
owned by: james
owned by: myers
mutable description: This Construct will allow for the detection and degredation of BPA in cells already containing the ER (K123003) biobrick. It works in two systems first. Together K123003 and K123009 produce a NOT gate where when Bisphenol A (BPA), Estrogen or any other estrogen mimic is in the system the product of K123003 dimerizes and attaches to the ERE site of K123009. This stops the production of TetR (R0040) by phsically stoping the Polymerase from transcribing it. This allows the pTet(C0040)promoter to turn on the production of RFP (E1010) for detection as well as BisdA(K123000) and BisdB(K123001)which allow for the degredation of BPA.
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: While BisdA and BisdB have only been shown to degrade BPA we have put in RFP to allow for the detection of other estrogen mimics as well.
star: true
creator: Jason Gardiner
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K123008
component: BBa_E1010
component: BBa_R0040
component: BBa_K123002
sequence annotation: BBa_K123002
sequence annotation: BBa_R0040
sequence annotation: BBa_E1010
sequence annotation: BBa_K123008
sequence: https://synbiohub.org/public/igem/BBa_K123009_sequence/1

BBa_K123008
type: Component
persistent identity: component2267240
display id: component2267240
version: 1
top level: BBa_K123009
definition: BBa_K123008
access: public

BBa_E1010
type: Component
persistent identity: component2267227
display id: component2267227
version: 1
top level: BBa_K123009
definition: mRFP1
access: public

BBa_R0040
type: Component
persistent identity: component2267220
display id: component2267220
version: 1
top level: BBa_K123009
definition: p(tetR)
access: public

BBa_K123002
type: Component
persistent identity: component2267219
display id: component2267219
version: 1
top level: BBa_K123009
definition: BBa_K123002
access: public

BBa_K123002
type: SequenceAnnotation
persistent identity: annotation2267219
display id: annotation2267219
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267219/range2267219/1
component: BBa_K123002

https://synbiohub.org/public/igem/BBa_K123009/annotation2267219/range2267219/1
type: Range
persistent identity: range2267219
display id: range2267219
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 1
end: 742
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2267220
display id: annotation2267220
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267220/range2267220/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123009/annotation2267220/range2267220/1
type: Range
persistent identity: range2267220
display id: range2267220
version: 1
top level: BBa_K123009
direction: direction/forward
start: 751
end: 804
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2267227
display id: annotation2267227
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267227/range2267227/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K123009/annotation2267227/range2267227/1
type: Range
persistent identity: range2267227
display id: range2267227
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 811
end: 1516
orientation: inline

BBa_K123008
type: SequenceAnnotation
persistent identity: annotation2267240
display id: annotation2267240
version: 1
top level: BBa_K123009
location: https://synbiohub.org/public/igem/BBa_K123009/annotation2267240/range2267240/1
component: BBa_K123008

https://synbiohub.org/public/igem/BBa_K123009/annotation2267240/range2267240/1
type: Range
persistent identity: range2267240
display id: range2267240
version: 1
top level: BBa_K123009
direction: direction/reverse
start: 1525
end: 3266
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_K123002
type: ComponentDefinition
persistent identity: BBa K123002
display id: BBa_K123002
version: 1
was generated by: iGEM to SBOL conversion
description: LacIQ ERE TetR
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element
owned by: james
owned by: myers
mutable description: This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase.
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.
star: true
creator: Jason Gardiner
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_I14032
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence annotation: ERE
sequence annotation: BBa_C0040
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: barcode
sequence: https://synbiohub.org/public/igem/BBa_K123002_sequence/1

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992720
display id: annotation1992720
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992720/range1992720/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992720/range1992720/1
type: Range
persistent identity: range1992720
display id: range1992720
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 1
end: 37
orientation: inline

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1992717
display id: annotation1992717
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992717/range1992717/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_K123002/annotation1992717/range1992717/1
type: Range
persistent identity: range1992717
display id: range1992717
version: 1
top level: BBa_K123002
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1992718
display id: annotation1992718
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992718/range1992718/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K123002/annotation1992718/range1992718/1
type: Range
persistent identity: range1992718
display id: range1992718
version: 1
top level: BBa_K123002
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1992719
display id: annotation1992719
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992719/range1992719/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K123002/annotation1992719/range1992719/1
type: Range
persistent identity: range1992719
display id: range1992719
version: 1
top level: BBa_K123002
direction: direction/forward
start: 26
end: 31
orientation: inline

ERE
type: SequenceAnnotation
persistent identity: annotation1992733
display id: annotation1992733
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992733/range1992733/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K123002/annotation1992733/range1992733/1
type: Range
persistent identity: range1992733
display id: range1992733
version: 1
top level: BBa_K123002
direction: direction/forward
start: 43
end: 57
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1992723
display id: annotation1992723
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992723/range1992723/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992723/range1992723/1
type: Range
persistent identity: range1992723
display id: range1992723
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 44
end: 703
orientation: inline

tetR
type: SequenceAnnotation
persistent identity: annotation1992721
display id: annotation1992721
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992721/range1992721/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K123002/annotation1992721/range1992721/1
type: Range
persistent identity: range1992721
display id: range1992721
version: 1
top level: BBa_K123002
direction: direction/forward
start: 47
end: 663
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1992722
display id: annotation1992722
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992722/range1992722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K123002/annotation1992722/range1992722/1
type: Range
persistent identity: range1992722
display id: range1992722
version: 1
top level: BBa_K123002
direction: direction/forward
start: 664
end: 697
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1992724
display id: annotation1992724
version: 1
top level: BBa_K123002
location: https://synbiohub.org/public/igem/BBa_K123002/annotation1992724/range1992724/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K123002/annotation1992724/range1992724/1
type: Range
persistent identity: range1992724
display id: range1992724
version: 1
top level: BBa_K123002
direction: direction/reverse
start: 704
end: 728
orientation: inline

BBa_K123008
type: ComponentDefinition
persistent identity: BBa K123008
display id: BBa_K123008
version: 1
was generated by: iGEM to SBOL conversion
description: BisdA+BisdB
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Bisphenolics
owned by: james
owned by: myers
mutable description: This part when used will allow the production of TetR to increase the production of BisdA and BisdB. When produced BisdA and BisdB will cause the degredation of Bisphenol A.
discontinued: false
dominant: false
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: N/A
star: false
creator: Jason Gardiner
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_R0040
component: BBa_K123001
component: BBa_K123000
sequence annotation: BBa_R0040
sequence annotation: BBa_K123000
sequence annotation: BBa_K123001
sequence: https://synbiohub.org/public/igem/BBa_K123008_sequence/1

BBa_R0040
type: Component
persistent identity: component1992756
display id: component1992756
version: 1
top level: BBa_K123008
definition: p(tetR)
access: public

BBa_K123001
type: Component
persistent identity: component1992767
display id: component1992767
version: 1
top level: BBa_K123008
definition: BisdB
access: public

BBa_R0040
type: Component
persistent identity: component1992762
display id: component1992762
version: 1
top level: BBa_K123008
definition: p(tetR)
access: public

BBa_K123000
type: Component
persistent identity: component1992761
display id: component1992761
version: 1
top level: BBa_K123008
definition: BisdA
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992756
display id: annotation1992756
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992756/range1992756/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123008/annotation1992756/range1992756/1
type: Range
persistent identity: range1992756
display id: range1992756
version: 1
top level: BBa_K123008
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_K123000
type: SequenceAnnotation
persistent identity: annotation1992761
display id: annotation1992761
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992761/range1992761/1
component: BBa_K123000

https://synbiohub.org/public/igem/BBa_K123008/annotation1992761/range1992761/1
type: Range
persistent identity: range1992761
display id: range1992761
version: 1
top level: BBa_K123008
direction: direction/reverse
start: 61
end: 390
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1992762
display id: annotation1992762
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992762/range1992762/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K123008/annotation1992762/range1992762/1
type: Range
persistent identity: range1992762
display id: range1992762
version: 1
top level: BBa_K123008
direction: direction/forward
start: 399
end: 452
orientation: inline

BBa_K123001
type: SequenceAnnotation
persistent identity: annotation1992767
display id: annotation1992767
version: 1
top level: BBa_K123008
location: https://synbiohub.org/public/igem/BBa_K123008/annotation1992767/range1992767/1
component: BBa_K123001

https://synbiohub.org/public/igem/BBa_K123008/annotation1992767/range1992767/1
type: Range
persistent identity: range1992767
display id: range1992767
version: 1
top level: BBa_K123008
direction: direction/reverse
start: 459
end: 1742
orientation: inline

BisdA
type: ComponentDefinition
persistent identity: BBa K123000
display id: BBa_K123000
version: 1
was generated by: iGEM to SBOL conversion
description: BisdA
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
mutable description: When used in conjunction with BisdB this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: These parts where designed to include a his tag to make it easier to view enzyme presence
star: true
creator: Jason Gardiner
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K123000_sequence/1

BisdB
type: ComponentDefinition
persistent identity: BBa K123001
display id: BBa_K123001
version: 1
was generated by: iGEM to SBOL conversion
description: BisdB
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: Sphingomonas sp. strain AO1
owned by: james
owned by: myers
mutable description: When used in conjunction with BisdA this enzyme can be used to degrade Bisphenol A. Activity has previously been recorded in E.coli by Miho Sasaki1 et al. 2005. Miho Sasaki1, Jun-ichi Maki, Ko-ichi Oshiman, Yoshinobu Matsumura1, and Tetsuaki Tsuchido.2005, Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp. strain AO1. Biodegredation 16: 449-459
discontinued: false
dominant: false
experience: experience/None
group u list: _241_
m user id: 0
owner id: 2742
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: When used with BisdA the degredation of Bisphenol A sharply increases
star: true
creator: Jason Gardiner
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K123001_sequence/1

https://synbiohub.org/public/igem/BBa_K123009_sequence/1
type: Sequence
persistent identity: BBa K123009 sequence
display id: BBa_K123009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcag…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123002_sequence/1
type: Sequence
persistent identity: BBa K123002 sequence
display id: BBa_K123002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagaggtcagagtgaccatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123008_sequence/1
type: Sequence
persistent identity: BBa K123008 sequence
display id: BBa_K123008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagatggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123000_sequence/1
type: Sequence
persistent identity: BBa K123000 sequence
display id: BBa_K123000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggccctcatatccaagtgactacccgcgatggggagatacgcgaactcgacgtcgcagcctccgggttcctgatggaagcccttcgcgacgccaatatcgacggcgtcgaggcgatgtgcggcggatgctgctcctgcgcgacctgccacgtctacatcgacgctgctcccgccgggaccctgccgccggtctcctccgacgaggagatgctgctttccggcctggtctcgacccccggacggtcgcggctctcctgccaaattccggtcacggccgaactggatggccttaagctcacgatcccgccggactccaccggttaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K123001_sequence/1
type: Sequence
persistent identity: BBa K123001 sequence
display id: BBa_K123001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccggcaaccctcagacactgccggtctttccagacctcgacatcttttcgcccgaatatgcctgcaaccgcgaaaaatacgcagcccgcgcccttagggactatccgctgcacttctataagccgctgaatttgtggatcgtcagcaagcacaaggatgtgcgctcggccttgttcactccgcaggtcttttcttctgtcgcgtttggcctgctccccccgcccgatgatatcgcgccgcgcgtgccggacctttacaccgacgtgcatctgccctcgatggacccgccggagcacaccaagctcagggttcccgtccagcaagccctcctccccggtcggctggtgggtaaggacgaggtcgttcgccggatcgccaacgaacttatcgataccttcatcgacaagggcgaatgcgatctcctccacgatttcagctacaaacttgcgctctacttgatcgtcgatctgctcgggatccccaaggaacgggcggaagattatcatcgctggtccaactgcttcttccagctgttcacccctaaggtaccggagcgcgccgacgcaagattcttcgtgccgatgccggaagaggtactgcggcagaactgggaaggcctggcggaggcgaacgactacctgcgcgaagtcgtcgagaaccttgatcgcaatcccggcaacaacatgctgtcaaacctgttgcagctgcgcgaaccggacggctcacgcacgatcacgatcagcgccaatgtttgcaacgcactggaatttggggcggctggtcatgacaccacggcaacgctgatcgcgcacctcacctatttcgtgctgaccacgccggacctcaaggacactcttaccgaggatccgtcgctgatccccgcggcgatttccgaaacgctgcgccgtcgctgtccggtcgacgggctgttccggcgaacgctgtcggatgtagaactatgcggccagaaaatcgaatccggatcgatcgtctacctcgatctcaccgctgcaaatcttgaccccgatgtattccccgaacctgagacgtttcgcctgaaccgcgacaatatcaaggaaatggtctctttcggcttcggtcgtcacgtctgcgcaggacagtacctgtcccgtatcgaagcaaaagcggcctacgaagaactgatgcgccgtattcccaacatgcgtctcgctgatggtttcaagctggaatacatgccttccgtggcgacgacggttctcaaggggcttccgctggtctgggacaaaaacaccggttaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z