LOCUS BBa_K125000 9047 bp DNA linear 05-Apr-2025 DEFINITION Broad host range BioBrick plasmid derived from pRL1383a with low to medium copy number ACCESSION BBa_K125000 VERSION BBa_K125000.1 FEATURES Location/Qualifiers promoter 8200..8230 /label=P5 promoter primer_bind 78..97 /label=VR primer_bind 8908..8927 /label=VF2 misc_feature 3476..3633 /label=oriT misc_feature 2819..3243 /label=oriR misc_feature 2664..8356 /label=RSF1010 derived region CDS 463..1255 /label=omega interposon CDS 933..1943 /label=spectinomycin marker promoter 8880..8898 /label=T7 promoter misc_feature 8616..8705 /label=cosN misc_feature 8831..8864 /label=loxP stem_loop 343..374 /label=T4 32 terminator stem_loop 2249..2280 /label=T4 32 terminator misc_feature 1..21 /label=BioBrick suffix misc_feature 9026..9047 /label=BioBrick prefix ORIGIN 1 tactagtagc ggccgctgca ggcttcctcg ctcactgact cgctgcgctc gtcgtgtgga 61 tgcggcgagc ggtatcagct cactcaaagg cggtaatgga tcctatagtg tcacctaaat 121 cgtatgcggc cgctcgtaag aggttccaac tttcaccata atgaaataag atcactaccg 181 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa 241 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca 301 tttcagtcag ttgctcaatg tacctataac cagaccgttc agaaaataaa aaaggggacc 361 tctagggtcc ccaattaatt agtaatataa tctattaaag gtcattcaaa aggtcatcca 421 ccggatcaat tcccctgctc gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc 481 cgatccttgg agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc 541 ttgacccgca gttgcaaacc ctcactgatc cgcccgttcc atacagaagc tgggcgaaca 601 aacgatgctc gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca 661 ccggcaagcg ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc 721 acagcacctt gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg 781 aaaccttgcg ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg 841 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct 901 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct 961 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt 1021 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc 1081 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa 1141 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag 1201 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg 1261 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa 1321 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc 1381 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca 1441 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca 1501 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa 1561 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg 1621 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc 1681 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg 1741 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc 1801 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag 1861 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga 1921 tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg 1981 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca 2041 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga 2101 tatatcatga aaggctggct ttttcttgtt atcgcaatag ttggcgaagt aatcgcaaca 2161 tccgcattaa aatctagcga gggctttact aagctgatcc ggtggatgac cttttgaatg 2221 acctttaata gattatatta ctaattaatt ggggacccta gaggtcccct tttttatttt 2281 aacaaagaat acaagaaaat atttacaaaa aatcaattta acaattcctt aaaacatgca 2341 ggaattgacg atttaaacaa tattagcttt gaacaattct tatctctttt caatagctat 2401 aaattattta ataagtaagt taagggatgc aggtcgagta cccgtacaag cctaattgtg 2461 tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg 2521 gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca 2581 gcgaaccaat cagcagggtc atcgctagaa atcatcctta gcgaaagcta aggatttttt 2641 ttatctgaat taattctcgc gagtcctggc cgccacatga ggcatgttcc gcgtccttgc 2701 aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc tcagccgaaa 2761 tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta actgtcacga 2821 acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct gcaataactg 2881 tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt tggaaaaatc 2941 catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct tgtggggcgc 3001 tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt cgctgcgcta 3061 ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc ccgctaaacc 3121 ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc ggcctttccc 3181 cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg tcgatcattc 3241 agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg tggtcgcgtt 3301 caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg ctgttcacct 3361 tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg cgctcttgct 3421 gctgttccct tgcccgctcc cgctgaattt cggcattgat tcgcgctcgt tgttcttcga 3481 gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca ccccattgtt 3541 aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga ccctactttg 3601 taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc cacccttcgg 3661 gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac agcgaggcag 3721 catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc aatcggccag 3781 ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg atgaagtctt 3841 gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact actgggatgc 3901 tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat ttgccctgcc 3961 ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc agcacctgac 4021 cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg agaacccgca 4081 ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg ccgctcagtg 4141 gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga ccgaagcgct 4201 caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg ccaaccgggc 4261 attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg cgcagggcat 4321 cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag gccggggcat 4381 ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc agatcatcga 4441 cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg aagaaatcca 4501 gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg gcgacactgg 4561 ccgacgaagc ccggcaggtc atgagccaga ccaagcaggc cagcgaggcg caggcggcgg 4621 agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc aaagagttgc 4681 gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg tcgcgggggt 4741 ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg gtggtgctgc 4801 tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac ggctcgatct 4861 ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag gccgacagct 4921 caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgcaccca ccggccagat 4981 gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc tcaagcggat 5041 gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc atggtctggt 5101 gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg gccgggagcc 5161 tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc 5221 cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt acgacgccga 5281 cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca accgcaagga 5341 caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa 5401 gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg agcaggccca 5461 gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag 5521 ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc tggtcaagcg 5581 cttcggtcat gacctcagca agtgcgactt tatcgccgcg cagaagctgg ccagccgggg 5641 ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc tggcagagcg 5701 caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca tgggtctgcc 5761 cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc agcgaggcat 5821 ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac gcctgttata 5881 ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg cttcagggtc 5941 ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg gttatacgtc 6001 aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta accttgacgg 6061 ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt ttctcggtga 6121 ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc cctgacgctg 6181 tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag cgcaagttct 6241 ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc 6301 agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc 6361 aatggctacc cataagccta tcaatattct ggaggcgttc gcagcagcgc cgccaccgct 6421 ggactacgtt ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg 6481 tgccggtaaa tccatgctgg ccctgcaact ggccgcacag attgcaggcg ggccggatct 6541 gctggaggtg ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg aagacccgcc 6601 caccgccatt catcaccgcc tgcacgccct tggggcgcac ctcagcgccg aggaacggca 6661 agccgtggct gacggcctgc tgatccagcc gctgatcggc agcctgccca acatcatggc 6721 cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga 6781 cacgctgcgc cggttccaca tcgaggaaga aaacgccagc ggccccatgg cccaggtcat 6841 cggtcgcatg gaggccatcg ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc 6901 cagcaagggc gcggccatga tgggcgcagg cgaccagcag caggccagcc ggggcagctc 6961 ggtactggtc gataacatcc gctggcagtc ctacctgtcg agcatgacca gcgccgaggc 7021 cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa 7081 ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg gcggggtgct 7141 caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta 7201 agaacaagca cagcctcagc cacgtccggc acgacccggc gcactgtctg gcccccggcc 7261 tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg acgtatgact 7321 acggcgacgg caagcggatc gagttcagcg gcccggagcc gctgggcgct gatgatctgc 7381 gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt ggcccggaac 7441 ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg gaggccgtca 7501 ccgctgaatg ccatgtggtc aaaggtagct atcgggcgct ggcaaaggaa atcggggcag 7561 aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc ctttggaagg 7621 tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg 7681 ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg 7741 tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg 7801 aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc aaaaccggca 7861 aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt ggttcgacca 7921 tgcgcaagcg ccgcaagcgg gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga 7981 cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg gcaggctgac 8041 cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg cttattttcc 8101 tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca atattttgaa 8161 aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc tttgcttcca 8221 gatgtatgct cttctgctcc cgaacgccag caagacgtag cccagcgcgt cggccagctt 8281 gcaattcgcg ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 8341 acctgtcgtg ccagctcgcg agaattaatt caaaaaaagc ccgctcatta ggcgggctaa 8401 ttcgagggcg agacgaggac ttccattgtt cattccacgg acaaaaacag agaaaggaaa 8461 cgacagaggc caaaaagcct cgctttcagc acctgtcgtt tcctttcttt tcagagggta 8521 ttttaaataa aaacattaag ttatgacgaa gaagaacgga aacgccttaa accggaaaat 8581 tttcataaat agcgaaaacc cgcgaggtcg ccgccccgta acctgtcgga tcaccggaaa 8641 ggacccgtaa agtgataatg attatcatct acatatcaca acgtgcgtgg aggccatcaa 8701 accacgtcaa ataatcaatt atgacgcagg tatcgtatta attgatctgc atcaacttaa 8761 cgtaaaaaca acttcagaca atacaaatca gcgacactga atacggggca acctcatgtc 8821 ctcatcgcta ataacttcgt atagcataca ttatacgaag ttatattcga tgcggccgct 8881 aatacgactc actataggga gaagctttgc cacctgacgt ctaagaaacc attattatca 8941 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct 9001 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagag //