LOCUS BBa_K125010 6996 bp DNA linear 17-May-2024 DEFINITION Broad-Host-Range RSF1010 derived plasmid ACCESSION BBa_K125010 VERSION BBa_K125010.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 66..102 /label=P(Lac) IQ promoter 68..73 /label=-35 promoter 91..96 /label=-10 promoter 66..102 /label=BBa_I14032 misc_feature 115..118 /label=conserved RBS 111..122 /label=BBa_B0034 misc_feature 131..167 /label=I14032 misc_feature 176..190 /label=B0030 CDS 197..1168 /label=RepB' promoter 1158..1209 /label=Promoter[EFRepAC] CDS 1229..3356 /label=[EFRepAC] stem_loop 3483..3515 /label=Native Terminator CDS 131..3515 /label=BBa_K125510 misc_feature 66..3515 /label=BBa_K125800 misc_feature 3524..3560 /label=P(Lac) IQ promoter 3526..3531 /label=-35 promoter 3549..3554 /label=-10 promoter 3524..3560 /label=BBa_I14032 misc_feature 3573..3576 /label=conserved RBS 3569..3580 /label=BBa_B0034 misc_feature 3589..3625 /label=P(Lac) IQ promoter 3591..3596 /label=-35 promoter 3614..3619 /label=-10 promoter 3589..3625 /label=BBa_I14032 misc_feature 3638..3641 /label=conserved RBS 3634..3645 /label=BBa_B0034 CDS 3652..4443 /label=BBa_K125520 misc_feature 3589..4443 /label=BBa_K125530 oriT 4452..4550 /label=BBa_K125320 misc_feature 4559..4579 /label=Suffix misc_feature 4560..4565 /label=SpeI misc_feature 4574..4579 /label=PstI misc_feature 4567..4574 /label=NotI misc_feature 4559..4579 /label=BBa_G00001 misc_feature 4580..4584 /label=Nt.BstNB I recognition site misc_feature 4585..4587 /label=Arbitrary sequence (could be any bases) misc_feature 4588..4592 /label=Topoisomerase I recognition site misc_feature 4588..4588 /label=Generated T overhang misc_feature 4580..4592 /label=BBa_B0044 misc_feature 4594..4596 /label=stop codon (+2 frame) misc_feature 4593..4595 /label=stop codon (-1 frame) misc_feature 4598..4600 /label=stop codon (+3 frame) misc_feature 4602..4604 /label=stop codon (+1 frame) misc_feature 4601..4603 /label=stop codon (-2 frame) misc_feature 4597..4599 /label=stop codon (-3 frame) misc_feature 4593..4604 /label=BBa_B0042 stem_loop 4615..4646 /label=stem loop misc_feature 4608..4647 /label=identical to E. coli MG1655 between tonB and yciA terminator 4605..4673 /label=BBa_B0054 primer_bind 4674..4693 /label=VR misc_feature 4674..4693 /label=BBa_G00102 stem_loop 4707..4726 /label=stem_loop terminator 4694..4734 /label=BBa_B0062 misc_feature 4735..4740 /label=NheI site misc_feature 4735..4740 /label=BBa_B0045 CDS 4741..5604 /label=ampicillin resistance RBS 5609..5613 /label=ampR RBS promoter 5622..5627 /label=ampR -10 promoter 5648..5653 /label=ampR -35 misc_feature 4741..5683 /label=BBa_P1006 misc_feature 5684..5689 /label=NheI site misc_feature 5684..5689 /label=BBa_B0045 stem_loop 5698..5732 /label=stem_loop terminator 5690..5761 /label=BBa_B0053 primer_bind 5762..5781 /label=VF2 primer 5762..5781 /label=BBa_G00100 stem_loop 5817..5838 /label=stem loop from bacteriophage T3 misc_feature 5809..5847 /label=identical to bacteriophage T3 terminator 5782..5859 /label=BBa_B0055 misc_feature 5861..5863 /label=stop codon (+2 frame) misc_feature 5860..5862 /label=stop codon (-1 frame) misc_feature 5865..5867 /label=stop codon (+3 frame) misc_feature 5869..5871 /label=stop codon (+1 frame) misc_feature 5868..5870 /label=stop codon (-2 frame) misc_feature 5864..5866 /label=stop codon (-3 frame) misc_feature 5860..5871 /label=BBa_B0042 misc_feature 5872..5876 /label=Topoisomerase I recognition site misc_feature 5880..5884 /label=Nt.BstNB I recognition site misc_feature 5877..5879 /label=Arbitrary sequence (could be any bases) misc_feature 5876..5876 /label=Generated T overhang misc_feature 5872..5884 /label=BBa_B0043 misc_feature 5885..5890 /label=EcoRI site misc_feature 5900..5905 /label=XbaI site misc_feature 5885..5906 /label=Prefix misc_feature 5891..5898 /label=NotI site misc_feature 5885..5906 /label=BBa_G00000 promoter 5947..5952 /label=-35 promoter 5970..5975 /label=-10 CDS 6014..6322 /label=ccdB misc_feature 5907..6322 /label=BBa_P1016 misc_feature 6338..6952 /label=rep (pMB1) promoter 6349..6354 /label=RNAII transcript promoter -35 sequence misc_feature 6349..6937 /label=origin of replication promoter 6370..6375 /label=RNAII transcript promoter -10 sequence misc_feature 6385..6937 /label=RNAII transcript misc_feature 6388..6495 /label=RNAI transcript promoter 6504..6509 /label=RNAI transcript promoter -10 promoter 6526..6531 /label=RNAI transcript promoter -35 misc_feature 6937..6937 /label=ORI misc_feature 6323..6996 /label=BBa_I50022 misc_feature 4559..6996 /label=BBa_I51020 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagagtggtg caaaaccttt cgcggtatgg catgatagcg cctactagag aaagaggaga 121 aatactagag tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagattaa 181 agaggagaaa tactagatga agaacgacag gactttgcag gccataggcc gacagctcaa 241 ggccatgggc tgtgagcgct tcgatatcgg cgtcagggac gcacccaccg gccagatgat 301 gaaccgggaa tggtcagccg ccgaagtgct ccagaacacg ccatggctca agcggatgaa 361 tgcccagggc aatgacgtgt atatcaggcc cgccgagcag gagcggcatg gtctggtgct 421 ggtggacgac ctcagcgagt ttgacctgga tgacatgaaa gccgagggcc gggagcctgc 481 cctggtagtg gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg ccgacgccgc 541 aggcggtgaa cttcgggggc agattgcccg gacgctggcc agcgagtacg acgccgaccc 601 ggccagcgcc gacagccgcc actatggccg cttggcgggc ttcaccaacc gcaaggacaa 661 gcacaccacc cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca agggcaagac 721 cgccaccgct ggcccggcgc tggtgcagca ggctggccag cagatcgagc aggcccagcg 781 gcagcaggag aaggcccgca ggctggccag cctcgaactg cccgagcggc agcttagccg 841 ccaccggcgc acggcgctgg acgagtaccg cagcgagatg gccgggctgg tcaagcgctt 901 cggtcatgac ctcagcaagt gcgactttat cgccgcgcag aagctggcca gccggggccg 961 cagtgccgag gaaatcggca aggccatggc cgaggccagc ccagcgctgg cagagcgcaa 1021 gcccggccac gaagcggatt acatcgagcg caccgtcagc aaggtcatgg gtctgcccag 1081 cgtccagctt gcgcgggccg agctggcacg ggcaccggca ccccgccagc gaggcatgga 1141 caggggcggg ccagatttca gcatgtagtg cttgcgttgg tactcacgcc tgttatacta 1201 tgagtactca cgcacagaag ggggttttat ggaatacgaa aaaagcgctt cagggtcggt 1261 ctacctgatc aaaagtgaca agggctattg gttgcccggt ggctttggtt atacgtcaaa 1321 caaggccgag gctggccgct tttcagtcgc tgatatggcc agccttaacc ttgacggctg 1381 caccttgtcc ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc tcggtgactg 1441 atatgaaaga ccaaaaggac aagcagaccg gcgacctgct ggccagccct gacgctgtac 1501 gccaagcgcg atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc aagttctggc 1561 tgaccgacga cgaatacgag gcgctgcgcg agtgcctgga agaactcaga gcggcgcagg 1621 gcgggggtag tgaccccgcc agcgcctaac caccaactgc ctgcaaagga ggcaatcaat 1681 ggctacccat aagcctatca atattctgga ggcgttcgca gcagcgccgc caccgctgga 1741 ctacgttttg cccaacatgg tggccggtac ggtcggggcg ctggtgtcgc ccggtggtgc 1801 cggtaaatcc atgctggccc tgcaactggc cgcacagatt gcaggcgggc cggatctgct 1861 ggaggtgggc gaactgccca ccggcccggt gatctacctg cccgccgaag acccgcccac 1921 cgccattcat caccgcctgc acgcccttgg ggcgcacctc agcgccgagg aacggcaagc 1981 cgtggctgac ggcctgctga tccagccgct gatcggcagc ctgcccaaca tcatggcccc 2041 ggagtggttc gacggcctca agcgcgccgc cgagggccgc cgcctgatgg tgctggacac 2101 gctgcgccgg ttccacatcg aggaagaaaa cgccagcggc cccatggccc aggtcatcgg 2161 tcgcatggag gccatcgccg ccgataccgg gtgctctatc gtgttcctgc accatgccag 2221 caagggcgcg gccatgatgg gcgcaggcga ccagcagcag gccagccggg gcagctcggt 2281 actggtcgat aacatccgct ggcagtccta cctgtcgagc atgaccagcg ccgaggccga 2341 ggaatggggt gtggacgacg accagcgccg gttcttcgtc cgcttcggtg tgagcaaggc 2401 caactatggc gcaccgttcg ctgatcggtg gttcaggcgg catgacggcg gggtgctcaa 2461 gcccgccgtg ctggagaggc agcgcaagag caagggggtg ccccgtggtg aagcctaaga 2521 acaagcacag cctcagccac gtccggcacg acccggcgca ctgtctggcc cccggcctgt 2581 tccgtgccct caagcggggc gagcgcaagc gcagcaagct ggacgtgacg tatgactacg 2641 gcgacggcaa gcggatcgag ttcagcggcc cggagccgct gggcgctgat gatctgcgca 2701 tcctgcaagg gctggtggcc atggctgggc ctaatggcct agtgcttggc ccggaaccca 2761 agaccgaagg cggacggcag ctccggctgt tcctggaacc caagtgggag gccgtcaccg 2821 ctgaatgcca tgtggtcaaa ggtagctatc gggcgctggc aaaggaaatc ggggcagagg 2881 tcgatagtgg tggggcgctc aagcacatac aggactgcat cgagcgcctt tggaaggtat 2941 ccatcatcgc ccagaatggc cgcaagcggc aggggtttcg gctgctgtcg gagtacgcca 3001 gcgacgaggc ggacgggcgc ctgtacgtgg ccctgaaccc cttgatcgcg caggccgtca 3061 tgggtggcgg ccagcatgtg cgcatcagca tggacgaggt gcgggcgctg gacagcgaaa 3121 ccgcccgcct gctgcaccag cggctgtgtg gctggatcga ccccggcaaa accggcaagg 3181 cttccataga taccttgtgc ggctatgtct ggccgtcaga ggccagtggt tcgaccatgc 3241 gcaagcgccg caagcgggtg cgcgaggcgt tgccggagct ggtcgcgctg ggctggacgg 3301 taaccgagtt cgcggcgggc aagtacgaca tcacccggcc caaggcggca ggctgacccc 3361 ccccactcta ttgtaaacaa gacattttta tcttttatat tcaatggctt attttcctgc 3421 taattggtaa taccatgaaa aataccatgc tcagaaaagg cttaacaata ttttgaaaaa 3481 ttgcctactg agcgctgccg cacagctcca taggctacta gagtggtgca aaacctttcg 3541 cggtatggca tgatagcgcc tactagagaa agaggagaaa tactagagtg gtgcaaaacc 3601 tttcgcggta tggcatgata gcgcctacta gagaaagagg agaaatacta gatgagggaa 3661 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat 3721 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag 3781 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg 3841 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc 3901 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct 3961 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag 4021 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt 4081 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt 4141 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag 4201 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg 4261 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc 4321 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca 4381 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa 4441 taatactaga ggaataaggg acagtgaaga aggaacaccc gctcgcgggt gggcctactt 4501 cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca tactagagta 4561 ctagtagcgg ccgctgcagg agtcactaag ggttagttag ttagattagc agaaagtcaa 4621 aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt ggggctcact 4681 caaaggcggt aatcagataa aaaaaatcct tagctttcgc taaggatgat ttctgctagc 4741 ttattaccaa tgcttaatca gggaggcacc tatctcagcg atctggcgat tacgttcgtc 4801 catagttgcc tggctccccg tcgtgtagat aactacgata cgggagggct taccatctgg 4861 ccccagggct gcaataatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat 4921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 4981 ccagtctatt aattgttgcc gggaagcgag agtaagaagt tcgccagtta agagtttgcg 5041 caacgttgtt gccatagcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 5101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcacccatgt tgtgcaaaaa 5161 agcggtaagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg ccgtgttatc 5221 actcatggtt atggcagcgc tgcataattc gcgtactgtc atgccgtccg taagatgctt 5281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 5341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca cagagcagaa ctttaaaagt 5401 gctcatcatt gggaaacgtt cttcggggcg aaaactctca agaatcttac cgctgttgag 5461 gtccagttcg atgtaaccca cacgtgcacc caactgatct tcggcatctt ttactttcac 5521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 5581 gacacggaaa tgttgaatac tcatattctt cctttttcaa tattattgaa gcatttatca 5641 gggttattgt ctcatgagcg gatagatatt tgaatgtatt taggctagct ccggcaaaaa 5701 aacgggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt 5761 ttgccacctg acgtctaaga aaaggaatat tcagcaattt gcccgtgccg aagaaaggcc 5821 cacccgtgaa ggtgagccag tgagttgatt gctacgtaat tagttagtta gcccttagtg 5881 actcgaattc gcggccgctt ctagagggct tactaaaagc cagataacag tatgcgtatt 5941 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa 6001 aaagaggtat gctatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct 6061 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct 6121 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat 6181 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat 6241 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct 6301 gatgttctgg ggaatataat aacccgtaga aaagatcaaa agatcttctt gagatccttt 6361 ttttctgcgc gtaatctgct acttgcaaac aaaaaaacca ccgctaccag cggtggtttg 6421 tttgccggat caagagctac caactctttt tccgaaggta actggcttta gcagagcgca 6481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 6541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 6601 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 6661 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 6721 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 6781 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 6841 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 6901 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 6961 acggttcctg gccttttgct ggccttttgc tcacat //