BBa_K125010
type: ComponentDefinition
persistent identity: BBa K125010
display id: BBa_K125010
version: 1
was generated by: iGEM to SBOL conversion
description: Broad-Host-Range RSF1010 derived plasmid
created: 2008-10-25T11:00:00Z
modified: 2015-07-09T01:36:31Z
mutable provenance: This part is derived from pRL1383a, an RSF1010 derived plasmid and the BioBrick Base Vector.
owned by: james
owned by: myers
mutable description: This Broad-Host-Range plasmid is derived from RSF1010. The replication proteins, the origin of vegetative replication, and the ''aadA'' gene were inserted into the Base Vector. The MPF system has been removed and replaced with the RP4 origin of transfer. This plasmid has been converted to BioBrick format and is also more compact that the original version. The replication proteins with the origin of vegetative replication allows for autonomous replication in a broad range of gram negative bacteria. The oriT allows for mobilization by the self-transmissible plasmid RP4. The ''aadA'' region allows resistance to Spectinomycin and Streptomycin. The Base Vector provides an insulated BioBrick cloning site containing a high copy origin and the ''ccdB'' death gene which are both removed when a part in cloned in. This allows one to make a lot of the plasmid for cloning, and a means for positive selection.
discontinued: true
dominant: false
experience: experience/None
group u list: _179_
m user id: 4206
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: PCR amplification was used to isolate the parts from pRL1383a. The oriT,lac promoter, and rbs were all synthesized.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
component: BBa_I14032
component: BBa_I51020
component: BBa_K125800
component: BBa_B0034
component: BBa_K125320
component: BBa_K125530
sequence annotation: BBa_I14032
sequence annotation: BBa_B0034
sequence annotation: BBa_K125800
sequence annotation: BBa_K125530
sequence annotation: BBa_K125320
sequence annotation: BBa_I51020
sequence: https://synbiohub.org/public/igem/BBa_K125010_sequence/1

BBa_I14032
type: Component
persistent identity: component2240159
display id: component2240159
version: 1
top level: BBa_K125010
definition: P(Lac) IQ
access: public

BBa_I51020
type: Component
persistent identity: component2240240
display id: component2240240
version: 1
top level: BBa_K125010
definition: BB base ve
access: public

BBa_K125800
type: Component
persistent identity: component2240155
display id: component2240155
version: 1
top level: BBa_K125010
definition: Rep
access: public

BBa_B0034
type: Component
persistent identity: component2240141
display id: component2240141
version: 1
top level: BBa_K125010
definition: BBa_B0034
access: public

BBa_I14032
type: Component
persistent identity: component2240139
display id: component2240139
version: 1
top level: BBa_K125010
definition: P(Lac) IQ
access: public

BBa_K125320
type: Component
persistent identity: component2240170
display id: component2240170
version: 1
top level: BBa_K125010
definition: OriT-RP4
access: public

BBa_K125530
type: Component
persistent identity: component2240169
display id: component2240169
version: 1
top level: BBa_K125010
definition: BBa_K125530
access: public

BBa_B0034
type: Component
persistent identity: component2240161
display id: component2240161
version: 1
top level: BBa_K125010
definition: BBa_B0034
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2240139
display id: annotation2240139
version: 1
top level: BBa_K125010
location: https://synbiohub.org/public/igem/BBa_K125010/annotation2240139/range2240139/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125010/annotation2240139/range2240139/1
type: Range
persistent identity: range2240139
display id: range2240139
version: 1
top level: BBa_K125010
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2240141
display id: annotation2240141
version: 1
top level: BBa_K125010
location: https://synbiohub.org/public/igem/BBa_K125010/annotation2240141/range2240141/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125010/annotation2240141/range2240141/1
type: Range
persistent identity: range2240141
display id: range2240141
version: 1
top level: BBa_K125010
direction: direction/reverse
start: 46
end: 57
orientation: inline

BBa_K125800
type: SequenceAnnotation
persistent identity: annotation2240155
display id: annotation2240155
version: 1
top level: BBa_K125010
location: https://synbiohub.org/public/igem/BBa_K125010/annotation2240155/range2240155/1
component: BBa_K125800

https://synbiohub.org/public/igem/BBa_K125010/annotation2240155/range2240155/1
type: Range
persistent identity: range2240155
display id: range2240155
version: 1
top level: BBa_K125010
direction: direction/reverse
start: 66
end: 3515
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation2240159
display id: annotation2240159
version: 1
top level: BBa_K125010
location: https://synbiohub.org/public/igem/BBa_K125010/annotation2240159/range2240159/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125010/annotation2240159/range2240159/1
type: Range
persistent identity: range2240159
display id: range2240159
version: 1
top level: BBa_K125010
direction: direction/reverse
start: 3524
end: 3560
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2240161
display id: annotation2240161
version: 1
top level: BBa_K125010
location: https://synbiohub.org/public/igem/BBa_K125010/annotation2240161/range2240161/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125010/annotation2240161/range2240161/1
type: Range
persistent identity: range2240161
display id: range2240161
version: 1
top level: BBa_K125010
direction: direction/reverse
start: 3569
end: 3580
orientation: inline

BBa_K125530
type: SequenceAnnotation
persistent identity: annotation2240169
display id: annotation2240169
version: 1
top level: BBa_K125010
location: https://synbiohub.org/public/igem/BBa_K125010/annotation2240169/range2240169/1
component: BBa_K125530

https://synbiohub.org/public/igem/BBa_K125010/annotation2240169/range2240169/1
type: Range
persistent identity: range2240169
display id: range2240169
version: 1
top level: BBa_K125010
direction: direction/reverse
start: 3589
end: 4443
orientation: inline

BBa_K125320
type: SequenceAnnotation
persistent identity: annotation2240170
display id: annotation2240170
version: 1
top level: BBa_K125010
location: https://synbiohub.org/public/igem/BBa_K125010/annotation2240170/range2240170/1
component: BBa_K125320

https://synbiohub.org/public/igem/BBa_K125010/annotation2240170/range2240170/1
type: Range
persistent identity: range2240170
display id: range2240170
version: 1
top level: BBa_K125010
direction: direction/reverse
start: 4452
end: 4550
orientation: inline

BBa_I51020
type: SequenceAnnotation
persistent identity: annotation2240240
display id: annotation2240240
version: 1
top level: BBa_K125010
location: https://synbiohub.org/public/igem/BBa_K125010/annotation2240240/range2240240/1
component: BBa_I51020

https://synbiohub.org/public/igem/BBa_K125010/annotation2240240/range2240240/1
type: Range
persistent identity: range2240240
display id: range2240240
version: 1
top level: BBa_K125010
direction: direction/reverse
start: 4559
end: 6996
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

BB base ve
type: ComponentDefinition
persistent identity: BBa I51020
display id: BBa_I51020
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick base vector (for constructing pSB**5 vectors)
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: This is a composite part generated from several basic parts obtained from a variety of sources.
owned by: james
owned by: myers
mutable description: This is a vector scaffold for constructing new BioBricks vectors. Information on the design is available at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Documented at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design Due to technical difficulties during synthesis, rather than including BBa_P1011 as designed, BBa_P1016 was synthesized as a component of this part. Hence, BBa_I51000 documents the original design and BBa_I51001 is the vector scaffold as synthesized. BBa_I52002 is the base vector as corrected from the synthesized construct.
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
component: BBa_B0044
component: BBa_G00001
component: BBa_G00000
component: BBa_B0043
component: BBa_B0042
component: BBa_B0055
component: BBa_I50022
component: BBa_P1016
component: BBa_B0045
component: BBa_B0062
component: BBa_G00102
component: BBa_B0054
component: BBa_G00100
component: BBa_B0053
component: BBa_P1006
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence annotation: BBa_B0045
sequence annotation: BBa_P1006
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_B0043
sequence annotation: BBa_G00000
sequence annotation: BBa_P1016
sequence annotation: BBa_I50022
sequence: https://synbiohub.org/public/igem/BBa_I51020_sequence/1

BBa_B0044
type: Component
persistent identity: component1959218
display id: component1959218
version: 1
top level: BB base ve
definition: TOPO
access: public

BBa_G00001
type: Component
persistent identity: component1959213
display id: component1959213
version: 1
top level: BB base ve
definition: Suffix
access: public

BBa_G00000
type: Component
persistent identity: component1959263
display id: component1959263
version: 1
top level: BB base ve
definition: Prefix
access: public

BBa_B0043
type: Component
persistent identity: component1959259
display id: component1959259
version: 1
top level: BB base ve
definition: TOPO
access: public

BBa_B0042
type: Component
persistent identity: component1959254
display id: component1959254
version: 1
top level: BB base ve
definition: TSS
access: public

BBa_B0055
type: Component
persistent identity: component1959247
display id: component1959247
version: 1
top level: BB base ve
definition: BBa_B0055
access: public

BBa_I50022
type: Component
persistent identity: component1959277
display id: component1959277
version: 1
top level: BB base ve
definition: pUC19 ori
access: public

BBa_P1016
type: Component
persistent identity: component1959267
display id: component1959267
version: 1
top level: BB base ve
definition: ccdB
access: public

BBa_B0045
type: Component
persistent identity: component1959233
display id: component1959233
version: 1
top level: BB base ve
definition: NheI
access: public

BBa_B0062
type: Component
persistent identity: component1959231
display id: component1959231
version: 1
top level: BB base ve
definition: BBa_B0062
access: public

BBa_G00102
type: Component
persistent identity: component1959229
display id: component1959229
version: 1
top level: BB base ve
definition: VR
access: public

BBa_B0054
type: Component
persistent identity: component1959228
display id: component1959228
version: 1
top level: BB base ve
definition: BBa_B0054
access: public

BBa_G00100
type: Component
persistent identity: component1959244
display id: component1959244
version: 1
top level: BB base ve
definition: VF2
access: public

BBa_B0053
type: Component
persistent identity: component1959242
display id: component1959242
version: 1
top level: BB base ve
definition: BBa_B0053
access: public

BBa_B0045
type: Component
persistent identity: component1959240
display id: component1959240
version: 1
top level: BB base ve
definition: NheI
access: public

BBa_P1006
type: Component
persistent identity: component1959238
display id: component1959238
version: 1
top level: BB base ve
definition: ampR
access: public

BBa_B0042
type: Component
persistent identity: component1959225
display id: component1959225
version: 1
top level: BB base ve
definition: TSS
access: public

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1959213
display id: annotation1959213
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959213/range1959213/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51020/annotation1959213/range1959213/1
type: Range
persistent identity: range1959213
display id: range1959213
version: 1
top level: BB base ve
direction: direction/reverse
start: 1
end: 21
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1959218
display id: annotation1959218
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959218/range1959218/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51020/annotation1959218/range1959218/1
type: Range
persistent identity: range1959218
display id: range1959218
version: 1
top level: BB base ve
direction: direction/reverse
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1959225
display id: annotation1959225
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959225/range1959225/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51020/annotation1959225/range1959225/1
type: Range
persistent identity: range1959225
display id: range1959225
version: 1
top level: BB base ve
direction: direction/reverse
start: 35
end: 46
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1959228
display id: annotation1959228
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959228/range1959228/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51020/annotation1959228/range1959228/1
type: Range
persistent identity: range1959228
display id: range1959228
version: 1
top level: BB base ve
direction: direction/reverse
start: 47
end: 115
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1959229
display id: annotation1959229
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959229/range1959229/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51020/annotation1959229/range1959229/1
type: Range
persistent identity: range1959229
display id: range1959229
version: 1
top level: BB base ve
direction: direction/reverse
start: 116
end: 135
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1959231
display id: annotation1959231
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959231/range1959231/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51020/annotation1959231/range1959231/1
type: Range
persistent identity: range1959231
display id: range1959231
version: 1
top level: BB base ve
direction: direction/reverse
start: 136
end: 176
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1959233
display id: annotation1959233
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959233/range1959233/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51020/annotation1959233/range1959233/1
type: Range
persistent identity: range1959233
display id: range1959233
version: 1
top level: BB base ve
direction: direction/reverse
start: 177
end: 182
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation1959238
display id: annotation1959238
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959238/range1959238/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51020/annotation1959238/range1959238/1
type: Range
persistent identity: range1959238
display id: range1959238
version: 1
top level: BB base ve
direction: direction/reverse
start: 183
end: 1125
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1959240
display id: annotation1959240
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959240/range1959240/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51020/annotation1959240/range1959240/1
type: Range
persistent identity: range1959240
display id: range1959240
version: 1
top level: BB base ve
direction: direction/reverse
start: 1126
end: 1131
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1959242
display id: annotation1959242
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959242/range1959242/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51020/annotation1959242/range1959242/1
type: Range
persistent identity: range1959242
display id: range1959242
version: 1
top level: BB base ve
direction: direction/reverse
start: 1132
end: 1203
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1959244
display id: annotation1959244
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959244/range1959244/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51020/annotation1959244/range1959244/1
type: Range
persistent identity: range1959244
display id: range1959244
version: 1
top level: BB base ve
direction: direction/reverse
start: 1204
end: 1223
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1959247
display id: annotation1959247
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959247/range1959247/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51020/annotation1959247/range1959247/1
type: Range
persistent identity: range1959247
display id: range1959247
version: 1
top level: BB base ve
direction: direction/reverse
start: 1224
end: 1301
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1959254
display id: annotation1959254
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959254/range1959254/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51020/annotation1959254/range1959254/1
type: Range
persistent identity: range1959254
display id: range1959254
version: 1
top level: BB base ve
direction: direction/reverse
start: 1302
end: 1313
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1959259
display id: annotation1959259
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959259/range1959259/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51020/annotation1959259/range1959259/1
type: Range
persistent identity: range1959259
display id: range1959259
version: 1
top level: BB base ve
direction: direction/reverse
start: 1314
end: 1326
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1959263
display id: annotation1959263
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959263/range1959263/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51020/annotation1959263/range1959263/1
type: Range
persistent identity: range1959263
display id: range1959263
version: 1
top level: BB base ve
direction: direction/reverse
start: 1327
end: 1348
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1959267
display id: annotation1959267
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959267/range1959267/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51020/annotation1959267/range1959267/1
type: Range
persistent identity: range1959267
display id: range1959267
version: 1
top level: BB base ve
direction: direction/reverse
start: 1349
end: 1764
orientation: inline

BBa_I50022
type: SequenceAnnotation
persistent identity: annotation1959277
display id: annotation1959277
version: 1
top level: BB base ve
location: https://synbiohub.org/public/igem/BBa_I51020/annotation1959277/range1959277/1
component: BBa_I50022

https://synbiohub.org/public/igem/BBa_I51020/annotation1959277/range1959277/1
type: Range
persistent identity: range1959277
display id: range1959277
version: 1
top level: BB base ve
direction: direction/reverse
start: 1765
end: 2438
orientation: inline

OriT-RP4
type: ComponentDefinition
persistent identity: BBa K125320
display id: BBa_K125320
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-RP4 (Origin of transfer for the RP4 plasmid)
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: RP4 NCBI accession number NC_001621
owned by: james
owned by: myers
mutable description: Relaxation region of self-transmissible plasmid RP4. When this part is added to a plasmid, it is transferred by RP4 through conjugation.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is similar to J01003, which has no experience and is much longer. In the process of designing and testing this part, it will be compared to J01003.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000724
role: partType/Conjugation
sequence: https://synbiohub.org/public/igem/BBa_K125320_sequence/1

Rep
type: ComponentDefinition
persistent identity: BBa K125800
display id: BBa_K125800
version: 1
was generated by: iGEM to SBOL conversion
description: Origin of Replication with Replication Proteins
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
owned by: james
owned by: myers
mutable description: oriV and the corresponding Rep proteins (from RSF1010) facilitate autonomous replicattion in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301. There are three replication proteins. Two of these proteins, RepA, a helicase, and RepC, an oriV binding protein are found on the same operon (E/F/repA/repC) which also includes a hypothetical protein, and an auto-regulatory protein: repressor F. Regulation at the level of translation is also found in this operon. A functional RepC requires the upstream translation of RepA (Scholtz 1988). A G+C rich region with dyad-symmetry followed by an A+T rich region is located at the end of the operon (E/F/repA/repC) which may be a rho-independent transcription terminator (Scholtz 1988). When pRL1383a was designed, an additional terminator was placed downstream of the (E/F/repA/repC) operon (Wolk 2007). RepB???, a functional subunit of the MobA/RepB dimer (Katashkina 2007, Scholtz 1988) acts as a primase during vegetative replication. RepB??? is under the same promoter as MobA and the product is a dimer in which the N-terminal domain is active in mobilization and the C-terminal domain (RepB???) is functional in primer synthesis at the origin of replication. In the past, the isolation of RepB???, in an attempt to make a non-mobilizable mutant of RSF1010, required that repB??? be put under another promoter, PlacUV5lacI, for successful replicative capability (Katashkina 2007). The choice of promoters is important because plasmid copy number is largely determined by the auto-regulatory function of mobilization proteins MobC and MobA, so the promoter chosen must also have some regulatory capabilities (Katashkina 2007…
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: RepB' is placed under the regulation of I14032, a lac promoter and an RBS with an efficiency of 0.6. The copy number will have to be determined. RSF1010 plasmids are low copy (~10 per cell in PCC6803 and ~12 per cell in ''E. coli''. Katashinka (2007) suggests that if RepB' is not regulated, the copy number will triple.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K125510
component: BBa_B0034
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_B0034
sequence annotation: BBa_K125510
sequence: https://synbiohub.org/public/igem/BBa_K125800_sequence/1

BBa_K125510
type: Component
persistent identity: component1988073
display id: component1988073
version: 1
top level: Rep
definition: Rep
access: public

BBa_B0034
type: Component
persistent identity: component1988066
display id: component1988066
version: 1
top level: Rep
definition: BBa_B0034
access: public

BBa_I14032
type: Component
persistent identity: component1988064
display id: component1988064
version: 1
top level: Rep
definition: P(Lac) IQ
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1988064
display id: annotation1988064
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125800/annotation1988064/range1988064/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125800/annotation1988064/range1988064/1
type: Range
persistent identity: range1988064
display id: range1988064
version: 1
top level: Rep
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1988066
display id: annotation1988066
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125800/annotation1988066/range1988066/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125800/annotation1988066/range1988066/1
type: Range
persistent identity: range1988066
display id: range1988066
version: 1
top level: Rep
direction: direction/reverse
start: 46
end: 57
orientation: inline

BBa_K125510
type: SequenceAnnotation
persistent identity: annotation1988073
display id: annotation1988073
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125800/annotation1988073/range1988073/1
component: BBa_K125510

https://synbiohub.org/public/igem/BBa_K125800/annotation1988073/range1988073/1
type: Range
persistent identity: range1988073
display id: range1988073
version: 1
top level: Rep
direction: direction/reverse
start: 66
end: 3450
orientation: inline

BBa_K125530
type: ComponentDefinition
persistent identity: BBa K125530
display id: BBa_K125530
version: 1
was generated by: iGEM to SBOL conversion
description: aadA gene with lacIQ promoter and RBS
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part was amplified from pRL1383a, an RSF1010 derived plasmid.
owned by: james
owned by: myers
mutable description: The ''aadA'' gene confers resistance to Spectinomycin and Streptomycin. The lacIQ promoter and an [[Part:BBa_B0034|RBS] were added. This part is functional without a terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part will be tested on varying concentrations of antibiotics.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K125520
component: BBa_B0034
component: BBa_I14032
sequence annotation: BBa_I14032
sequence annotation: BBa_B0034
sequence annotation: BBa_K125520
sequence: https://synbiohub.org/public/igem/BBa_K125530_sequence/1

BBa_K125520
type: Component
persistent identity: component1988107
display id: component1988107
version: 1
top level: BBa_K125530
definition: aada
access: public

BBa_B0034
type: Component
persistent identity: component1988106
display id: component1988106
version: 1
top level: BBa_K125530
definition: BBa_B0034
access: public

BBa_I14032
type: Component
persistent identity: component1988104
display id: component1988104
version: 1
top level: BBa_K125530
definition: P(Lac) IQ
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1988104
display id: annotation1988104
version: 1
top level: BBa_K125530
location: https://synbiohub.org/public/igem/BBa_K125530/annotation1988104/range1988104/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125530/annotation1988104/range1988104/1
type: Range
persistent identity: range1988104
display id: range1988104
version: 1
top level: BBa_K125530
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1988106
display id: annotation1988106
version: 1
top level: BBa_K125530
location: https://synbiohub.org/public/igem/BBa_K125530/annotation1988106/range1988106/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125530/annotation1988106/range1988106/1
type: Range
persistent identity: range1988106
display id: range1988106
version: 1
top level: BBa_K125530
direction: direction/reverse
start: 46
end: 57
orientation: inline

BBa_K125520
type: SequenceAnnotation
persistent identity: annotation1988107
display id: annotation1988107
version: 1
top level: BBa_K125530
location: https://synbiohub.org/public/igem/BBa_K125530/annotation1988107/range1988107/1
component: BBa_K125520

https://synbiohub.org/public/igem/BBa_K125530/annotation1988107/range1988107/1
type: Range
persistent identity: range1988107
display id: range1988107
version: 1
top level: BBa_K125530
direction: direction/reverse
start: 64
end: 855
orientation: inline

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50022
display id: BBa_I50022
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal pUC19-derived high copy replication origin
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: pSB1A3
owned by: james
owned by: myers
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: rep (pMB1)
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: origin of replication
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: RNAI transcript
sequence annotation: RNAI transcript promoter -10
sequence annotation: RNAI transcript promoter -35
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_I50022_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1959117
display id: annotation1959117
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1
type: Range
persistent identity: range1959117
display id: range1959117
version: 1
top level: pUC19 ori
direction: direction/forward
start: 16
end: 630
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1959118
display id: annotation1959118
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1
type: Range
persistent identity: range1959118
display id: range1959118
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 32
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1959119
display id: annotation1959119
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1
type: Range
persistent identity: range1959119
display id: range1959119
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 615
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1959120
display id: annotation1959120
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1
type: Range
persistent identity: range1959120
display id: range1959120
version: 1
top level: pUC19 ori
direction: direction/forward
start: 48
end: 53
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1959121
display id: annotation1959121
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1
type: Range
persistent identity: range1959121
display id: range1959121
version: 1
top level: pUC19 ori
direction: direction/forward
start: 63
end: 615
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1959122
display id: annotation1959122
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1
type: Range
persistent identity: range1959122
display id: range1959122
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 66
end: 173
orientation: inline

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1959123
display id: annotation1959123
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1
type: Range
persistent identity: range1959123
display id: range1959123
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 182
end: 187
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1959124
display id: annotation1959124
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1
type: Range
persistent identity: range1959124
display id: range1959124
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 204
end: 209
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1959125
display id: annotation1959125
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1
type: Range
persistent identity: range1959125
display id: range1959125
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 615
end: 615
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1016
display id: BBa_P1016
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cassette without ccdA-
created: 2007-01-29T12:00:00Z
modified: 2015-06-15T01:13:47Z
mutable provenance: This part is derived from BBa_P1010 but it lacks ccdA-.
owned by: james
owned by: myers
mutable description: This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 4206
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: -35
sequence annotation: -10
sequence annotation: ccdB
sequence: https://synbiohub.org/public/igem/BBa_P1016_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1910684
display id: annotation1910684
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
type: Range
persistent identity: range1910684
display id: range1910684
version: 1
top level: ccdB
direction: direction/forward
start: 41
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1910685
display id: annotation1910685
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
type: Range
persistent identity: range1910685
display id: range1910685
version: 1
top level: ccdB
direction: direction/forward
start: 64
end: 69
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1910686
display id: annotation1910686
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
type: Range
persistent identity: range1910686
display id: range1910686
version: 1
top level: ccdB
direction: direction/forward
start: 108
end: 416
orientation: inline

TSS
type: ComponentDefinition
persistent identity: BBa B0042
display id: BBa_B0042
version: 1
was generated by: iGEM to SBOL conversion
description: Translational stop sequence
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: stop codon (-1 frame)
sequence annotation: stop codon (+2 frame)
sequence annotation: stop codon (-3 frame)
sequence annotation: stop codon (+3 frame)
sequence annotation: stop codon (-2 frame)
sequence annotation: stop codon (+1 frame)
sequence: https://synbiohub.org/public/igem/BBa_B0042_sequence/1

stop codon (-1 frame)
type: SequenceAnnotation
persistent identity: annotation1797084
display id: annotation1797084
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
type: Range
persistent identity: range1797084
display id: range1797084
version: 1
top level: TSS
direction: direction/reverse
start: 1
end: 3
orientation: inline

stop codon (+2 frame)
type: SequenceAnnotation
persistent identity: annotation1797083
display id: annotation1797083
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
type: Range
persistent identity: range1797083
display id: range1797083
version: 1
top level: TSS
direction: direction/forward
start: 2
end: 4
orientation: inline

stop codon (-3 frame)
type: SequenceAnnotation
persistent identity: annotation1797088
display id: annotation1797088
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
type: Range
persistent identity: range1797088
display id: range1797088
version: 1
top level: TSS
direction: direction/reverse
start: 5
end: 7
orientation: inline

stop codon (+3 frame)
type: SequenceAnnotation
persistent identity: annotation1797085
display id: annotation1797085
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
type: Range
persistent identity: range1797085
display id: range1797085
version: 1
top level: TSS
direction: direction/forward
start: 6
end: 8
orientation: inline

stop codon (-2 frame)
type: SequenceAnnotation
persistent identity: annotation1797087
display id: annotation1797087
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
type: Range
persistent identity: range1797087
display id: range1797087
version: 1
top level: TSS
direction: direction/reverse
start: 9
end: 11
orientation: inline

stop codon (+1 frame)
type: SequenceAnnotation
persistent identity: annotation1797086
display id: annotation1797086
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
type: Range
persistent identity: range1797086
display id: range1797086
version: 1
top level: TSS
direction: direction/forward
start: 10
end: 12
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0043
display id: BBa_B0043
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (forward)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Nt.BstNB I recognition site
sequence: https://synbiohub.org/public/igem/BBa_B0043_sequence/1

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797101
display id: annotation1797101
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
type: Range
persistent identity: range1797101
display id: range1797101
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797104
display id: annotation1797104
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
type: Range
persistent identity: range1797104
display id: range1797104
version: 1
top level: TOPO
direction: direction/forward
start: 5
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797103
display id: annotation1797103
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
type: Range
persistent identity: range1797103
display id: range1797103
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797102
display id: annotation1797102
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
type: Range
persistent identity: range1797102
display id: range1797102
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0044
display id: BBa_B0044
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (reverse)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Nt.BstNB I recognition site
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence: https://synbiohub.org/public/igem/BBa_B0044_sequence/1

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797126
display id: annotation1797126
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
type: Range
persistent identity: range1797126
display id: range1797126
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797127
display id: annotation1797127
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
type: Range
persistent identity: range1797127
display id: range1797127
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797128
display id: annotation1797128
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
type: Range
persistent identity: range1797128
display id: range1797128
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797129
display id: annotation1797129
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
type: Range
persistent identity: range1797129
display id: range1797129
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 9
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Prefix
sequence annotation: EcoRI site
sequence annotation: NotI site
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Suffix
sequence annotation: SpeI
sequence annotation: NotI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa P1006
display id: BBa_P1006
version: 1
was generated by: iGEM to SBOL conversion
description: ampicillin resistance cassette, reverse orientation
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: This contains TetR, a terminator and then AmpR and a terminator
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: ampicillin resistance
sequence annotation: ampR RBS
sequence annotation: ampR -10
sequence annotation: ampR -35
sequence: https://synbiohub.org/public/igem/BBa_P1006_sequence/1

ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation1897231
display id: annotation1897231
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897231/range1897231/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1006/annotation1897231/range1897231/1
type: Range
persistent identity: range1897231
display id: range1897231
version: 1
top level: ampR
direction: direction/reverse
start: 1
end: 864
orientation: inline

ampR RBS
type: SequenceAnnotation
persistent identity: annotation1897232
display id: annotation1897232
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897232/range1897232/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_P1006/annotation1897232/range1897232/1
type: Range
persistent identity: range1897232
display id: range1897232
version: 1
top level: ampR
direction: direction/reverse
start: 869
end: 873
orientation: inline

ampR -10
type: SequenceAnnotation
persistent identity: annotation1897233
display id: annotation1897233
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897233/range1897233/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1006/annotation1897233/range1897233/1
type: Range
persistent identity: range1897233
display id: range1897233
version: 1
top level: ampR
direction: direction/reverse
start: 882
end: 887
orientation: inline

ampR -35
type: SequenceAnnotation
persistent identity: annotation1897234
display id: annotation1897234
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897234/range1897234/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1006/annotation1897234/range1897234/1
type: Range
persistent identity: range1897234
display id: range1897234
version: 1
top level: ampR
direction: direction/reverse
start: 908
end: 913
orientation: inline

Rep
type: ComponentDefinition
persistent identity: BBa K125510
display id: BBa_K125510
version: 1
was generated by: iGEM to SBOL conversion
description: Replication Proteins
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
owned by: james
owned by: myers
mutable description: Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part will be constructed with the origin of vegetative replication derived from an RSF1010 plasmid.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: I14032
sequence annotation: B0030
sequence annotation: RepB'
sequence annotation: Promoter[EFRepAC]
sequence annotation: [EFRepAC]
sequence annotation: Native Terminator
sequence: https://synbiohub.org/public/igem/BBa_K125510_sequence/1

I14032
type: SequenceAnnotation
persistent identity: annotation1974185
display id: annotation1974185
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974185/range1974185/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K125510/annotation1974185/range1974185/1
type: Range
persistent identity: range1974185
display id: range1974185
version: 1
top level: Rep
direction: direction/forward
start: 1
end: 37
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation1974186
display id: annotation1974186
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974186/range1974186/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K125510/annotation1974186/range1974186/1
type: Range
persistent identity: range1974186
display id: range1974186
version: 1
top level: Rep
direction: direction/forward
start: 46
end: 60
orientation: inline

RepB'
type: SequenceAnnotation
persistent identity: annotation1974187
display id: annotation1974187
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974187/range1974187/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K125510/annotation1974187/range1974187/1
type: Range
persistent identity: range1974187
display id: range1974187
version: 1
top level: Rep
direction: direction/forward
start: 67
end: 1038
orientation: inline

Promoter[EFRepAC]
type: SequenceAnnotation
persistent identity: annotation1974188
display id: annotation1974188
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974188/range1974188/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K125510/annotation1974188/range1974188/1
type: Range
persistent identity: range1974188
display id: range1974188
version: 1
top level: Rep
direction: direction/forward
start: 1028
end: 1079
orientation: inline

[EFRepAC]
type: SequenceAnnotation
persistent identity: annotation1974189
display id: annotation1974189
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974189/range1974189/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K125510/annotation1974189/range1974189/1
type: Range
persistent identity: range1974189
display id: range1974189
version: 1
top level: Rep
direction: direction/forward
start: 1099
end: 3226
orientation: inline

Native Terminator
type: SequenceAnnotation
persistent identity: annotation1974190
display id: annotation1974190
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974190/range1974190/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K125510/annotation1974190/range1974190/1
type: Range
persistent identity: range1974190
display id: range1974190
version: 1
top level: Rep
direction: direction/forward
start: 3353
end: 3385
orientation: inline

aada
type: ComponentDefinition
persistent identity: BBa K125520
display id: BBa_K125520
version: 1
was generated by: iGEM to SBOL conversion
description: aadA gene
created: 2008-10-25T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part was amplified from pRL1383a, an RSF1010 derived plasmid.
owned by: james
owned by: myers
mutable description: The ''aadA'' gene confers resistance to Spectinomycin and Streptomycin. The lacIQ promoter and an [[Part:BBa_B0034|RBS] were added. This part is functional without a terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part will be tested on varying concentrations of antibiotics.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K125520_sequence/1

https://synbiohub.org/public/igem/BBa_K125010_sequence/1
type: Sequence
persistent identity: BBa K125010 sequence
display id: BBa_K125010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgaagaacgacaggactttgcaggccataggccgacagctcaaggccatgggctgtgagcgcttcgatatcggcgtcagggacgcacccaccggccagatgatgaaccgggaatggtcagccgccgaagtgctccagaacacgccatggctcaagcggatgaatgcccagggcaatgacgtgtatatcaggcccgccgagcaggagcggcatggtctggtgctggtggacgacctcagcgagtttgacctggatgacatgaaagccgagggccgggagcctgccctggtagtggaaaccagcccgaagaactatcaggcatgggtcaaggtggccgacgccgcaggcggtgaacttcgggggcagattgcccggacgctggccagcgagtacgacgccgacccggccagcgccgacagccgccactatggccgcttggcgggcttcaccaaccgcaaggacaagcacaccacccgcgccggttatcagccgtgggtgctgctgcgtgaatccaagggcaagaccgccaccgctggcccggcgctggtgcagcaggctggccagcagatcgagcaggcccagcggcagcaggagaaggcccgcaggctggccagcctcgaactgcccgagcggcagcttagccgccaccggcgcacggcgctggacgagtaccgcagcgagatggccgggctggtcaagcgcttcggtcatgacctcagcaagtgcgactttatcgccgcgcagaagctggccagccggggccgcagtgccgaggaaatcggcaaggccatggccgaggccagcccagcgctggcagagcgcaagcccggccacgaagcggattacatcgagcgcaccgtcagcaaggtcatgggtctgcccagcgtccagcttgcgcgggccgagctggcacgggcaccggcaccccgccagcgaggcatggacaggggcgggccagatttcagcatgtagtgcttgcgttggtactcacgcctgttatactatgagtactcacgcacagaagggggttttatggaatacgaaaaaagcgcttcagggtcggtctacctgatcaaaagtgacaagggctattggttgcccggtggctttggttatacgtcaaacaaggccgaggctggccgcttttcagtcgctgatatggccagccttaaccttgacggctgcaccttgtccttgttccgcgaagacaagcctttcggccccggcaagtttctcggtgactgatatgaaagaccaaaaggacaagcagaccggcgacctgctggccagccctgacgctgtacgccaagcgcgatatgccgagcgcatgaaggccaaagggatgcgtcagcgcaagttctggctgaccgacgacgaatacgaggcgctgcgcgagtgcctggaagaactcagagcggcgcagggcgggggtagtgaccccgccagcgcctaaccaccaactgcctgcaaaggaggcaatcaatggctacccataagcctatcaatattctggaggcgttcgcagcagcgccgccaccgctggactacgttttgcccaacatggtggccggtacggtcggggcgctggtgtcgcccggtggtgccggtaaatccatgctggccctgcaactggccgcacagattgcaggcgggccggatctgctggaggtgggcgaactgcccaccggcccggtgatctacctgcccgccgaagacccgcccaccgccattcatcaccgcctgcacgcccttggggcgcacctcagcgccgaggaacggcaagccgtggctgacggcctgctga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I51020_sequence/1
type: Sequence
persistent identity: BBa I51020 sequence
display id: BBa_I51020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttaggctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125320_sequence/1
type: Sequence
persistent identity: BBa K125320 sequence
display id: BBa_K125320_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125800_sequence/1
type: Sequence
persistent identity: BBa K125800 sequence
display id: BBa_K125800_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgaagaacgacaggactttgcaggccataggccgacagctcaaggccatgggctgtgagcgcttcgatatcggcgtcagggacgcacccaccggccagatgatgaaccgggaatggtcagccgccgaagtgctccagaacacgccatggctcaagcggatgaatgcccagggcaatgacgtgtatatcaggcccgccgagcaggagcggcatggtctggtgctggtggacgacctcagcgagtttgacctggatgacatgaaagccgagggccgggagcctgccctggtagtggaaaccagcccgaagaactatcaggcatgggtcaaggtggccgacgccgcaggcggtgaacttcgggggcagattgcccggacgctggccagcgagtacgacgccgacccggccagcgccgacagccgccactatggccgcttggcgggcttcaccaaccgcaaggacaagcacaccacccgcgccggttatcagccgtgggtgctgctgcgtgaatccaagggcaagaccgccaccgctggcccggcgctggtgcagcaggctggccagcagatcgagcaggcccagcggcagcaggagaaggcccgcaggctggccagcctcgaactgcccgagcggcagcttagccgccaccggcgcacggcgctggacgagtaccgcagcgagatggccgggctggtcaagcgcttcggtcatgacctcagcaagtgcgactttatcgccgcgcagaagctggccagccggggccgcagtgccgaggaaatcggcaaggccatggccgaggccagcccagcgctggcagagcgcaagcccggccacgaagcggattacatcgagcgcaccgtcagcaaggtcatgggtctgcccagcgtccagcttgcgcgggccgagctggcacgggcaccggcaccccgccagcgaggcatggacaggggcgggccagatttcagcatgtagtgcttgcgttggtactcacgcctgttatactatgagtactcacgcacagaagggggttttatggaatacgaaaaaagcgcttcagggtcggtctacctgatcaaaagtgacaagggctattggttgcccggtggctttggttatacgtcaaacaaggccgaggctggccgcttttcagtcgctgatatggccagccttaaccttgacggctgcaccttgtccttgttccgcgaagacaagcctttcggccccggcaagtttctcggtgactgatatgaaagaccaaaaggacaagcagaccggcgacctgctggccagccctgacgctgtacgccaagcgcgatatgccgagcgcatgaaggccaaagggatgcgtcagcgcaagttctggctgaccgacgacgaatacgaggcgctgcgcgagtgcctggaagaactcagagcggcgcagggcgggggtagtgaccccgccagcgcctaaccaccaactgcctgcaaaggaggcaatcaatggctacccataagcctatcaatattctggaggcgttcgcagcagcgccgccaccgctggactacgttttgcccaacatggtggccggtacggtcggggcgctggtgtcgcccggtggtgccggtaaatccatgctggccctgcaactggccgcacagattgcaggcgggccggatctgctggaggtgggcgaactgcccaccggcccggtgatctacctgcccgccgaagacccgcccaccgccattcatcaccgcctgcacgcccttggggcgcacctcagcgccgaggaacggcaagccgtggctgacggcctgctgatccagccgctgatcggcagcctgcccaacatcatggccccggagtggttcgacggcctcaagcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125530_sequence/1
type: Sequence
persistent identity: BBa K125530 sequence
display id: BBa_K125530_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50022_sequence/1
type: Sequence
persistent identity: BBa I50022 sequence
display id: BBa_I50022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1016_sequence/1
type: Sequence
persistent identity: BBa P1016 sequence
display id: BBa_P1016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0042_sequence/1
type: Sequence
persistent identity: BBa B0042 sequence
display id: BBa_B0042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagttagttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0043_sequence/1
type: Sequence
persistent identity: BBa B0043 sequence
display id: BBa_B0043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttagtgactc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0044_sequence/1
type: Sequence
persistent identity: BBa B0044 sequence
display id: BBa_B0044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtcactaaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1006_sequence/1
type: Sequence
persistent identity: BBa P1006 sequence
display id: BBa_P1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125510_sequence/1
type: Sequence
persistent identity: BBa K125510 sequence
display id: BBa_K125510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgaagaacgacaggactttgcaggccataggccgacagctcaaggccatgggctgtgagcgcttcgatatcggcgtcagggacgcacccaccggccagatgatgaaccgggaatggtcagccgccgaagtgctccagaacacgccatggctcaagcggatgaatgcccagggcaatgacgtgtatatcaggcccgccgagcaggagcggcatggtctggtgctggtggacgacctcagcgagtttgacctggatgacatgaaagccgagggccgggagcctgccctggtagtggaaaccagcccgaagaactatcaggcatgggtcaaggtggccgacgccgcaggcggtgaacttcgggggcagattgcccggacgctggccagcgagtacgacgccgacccggccagcgccgacagccgccactatggccgcttggcgggcttcaccaaccgcaaggacaagcacaccacccgcgccggttatcagccgtgggtgctgctgcgtgaatccaagggcaagaccgccaccgctggcccggcgctggtgcagcaggctggccagcagatcgagcaggcccagcggcagcaggagaaggcccgcaggctggccagcctcgaactgcccgagcggcagcttagccgccaccggcgcacggcgctggacgagtaccgcagcgagatggccgggctggtcaagcgcttcggtcatgacctcagcaagtgcgactttatcgccgcgcagaagctggccagccggggccgcagtgccgaggaaatcggcaaggccatggccgaggccagcccagcgctggcagagcgcaagcccggccacgaagcggattacatcgagcgcaccgtcagcaaggtcatgggtctgcccagcgtccagcttgcgcgggccgagctggcacgggcaccggcaccccgccagcgaggcatggacaggggcgggccagatttcagcatgtagtgcttgcgttggtactcacgcctgttatactatgagtactcacgcacagaagggggttttatggaatacgaaaaaagcgcttcagggtcggtctacctgatcaaaagtgacaagggctattggttgcccggtggctttggttatacgtcaaacaaggccgaggctggccgcttttcagtcgctgatatggccagccttaaccttgacggctgcaccttgtccttgttccgcgaagacaagcctttcggccccggcaagtttctcggtgactgatatgaaagaccaaaaggacaagcagaccggcgacctgctggccagccctgacgctgtacgccaagcgcgatatgccgagcgcatgaaggccaaagggatgcgtcagcgcaagttctggctgaccgacgacgaatacgaggcgctgcgcgagtgcctggaagaactcagagcggcgcagggcgggggtagtgaccccgccagcgcctaaccaccaactgcctgcaaaggaggcaatcaatggctacccataagcctatcaatattctggaggcgttcgcagcagcgccgccaccgctggactacgttttgcccaacatggtggccggtacggtcggggcgctggtgtcgcccggtggtgccggtaaatccatgctggccctgcaactggccgcacagattgcaggcgggccggatctgctggaggtgggcgaactgcccaccggcccggtgatctacctgcccgccgaagacccgcccaccgccattcatcaccgcctgcacgcccttggggcgcacctcagcgccgaggaacggcaagccgtggctgacggcctgctgatccagccgctgatcggcagcctgcccaacatcatggccccggagtggttcgacggcctcaagcgcgccgccgagggccgccgcctgatggtgctggacacgctgcgccggttccacatcgaggaagaaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125520_sequence/1
type: Sequence
persistent identity: BBa K125520 sequence
display id: BBa_K125520_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z