LOCUS BBa_K125020 4841 bp DNA linear 03-May-2024 DEFINITION lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT ACCESSION BBa_K125020 VERSION BBa_K125020.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 66..102 /label=I14032 misc_feature 111..125 /label=B0030 CDS 132..1103 /label=RepB' promoter 1093..1144 /label=Promoter[EFRepAC] CDS 1164..3291 /label=[EFRepAC] stem_loop 3418..3450 /label=Native Terminator CDS 66..3450 /label=BBa_K125510 misc_feature 3461..3524 /label=RepC Binding Site misc_feature 3665..3810 /label=RepB Binding Site misc_feature 3811..3859 /label=RepA Binding Site misc_feature 3459..3882 /label=BBa_K125340 misc_feature 3891..3927 /label=P(Lac) IQ promoter 3893..3898 /label=-35 promoter 3916..3921 /label=-10 promoter 3891..3927 /label=BBa_I14032 misc_feature 3940..3943 /label=conserved RBS 3936..3947 /label=BBa_B0034 misc_feature 3956..4008 /label=slr2016 signal sequence misc_feature 3956..4008 /label=BBa_K125310 CDS 4017..4734 /label=GFP fusion brick misc_feature 4017..4017 /label=deletion of TG CDS 4017..4734 /label=BBa_K125500 oriT 4743..4841 /label=BBa_K125320 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagagtggtg caaaaccttt cgcggtatgg catgatagcg cctactagag attaaagagg 121 agaaatacta gatgaagaac gacaggactt tgcaggccat aggccgacag ctcaaggcca 181 tgggctgtga gcgcttcgat atcggcgtca gggacgcacc caccggccag atgatgaacc 241 gggaatggtc agccgccgaa gtgctccaga acacgccatg gctcaagcgg atgaatgccc 301 agggcaatga cgtgtatatc aggcccgccg agcaggagcg gcatggtctg gtgctggtgg 361 acgacctcag cgagtttgac ctggatgaca tgaaagccga gggccgggag cctgccctgg 421 tagtggaaac cagcccgaag aactatcagg catgggtcaa ggtggccgac gccgcaggcg 481 gtgaacttcg ggggcagatt gcccggacgc tggccagcga gtacgacgcc gacccggcca 541 gcgccgacag ccgccactat ggccgcttgg cgggcttcac caaccgcaag gacaagcaca 601 ccacccgcgc cggttatcag ccgtgggtgc tgctgcgtga atccaagggc aagaccgcca 661 ccgctggccc ggcgctggtg cagcaggctg gccagcagat cgagcaggcc cagcggcagc 721 aggagaaggc ccgcaggctg gccagcctcg aactgcccga gcggcagctt agccgccacc 781 ggcgcacggc gctggacgag taccgcagcg agatggccgg gctggtcaag cgcttcggtc 841 atgacctcag caagtgcgac tttatcgccg cgcagaagct ggccagccgg ggccgcagtg 901 ccgaggaaat cggcaaggcc atggccgagg ccagcccagc gctggcagag cgcaagcccg 961 gccacgaagc ggattacatc gagcgcaccg tcagcaaggt catgggtctg cccagcgtcc 1021 agcttgcgcg ggccgagctg gcacgggcac cggcaccccg ccagcgaggc atggacaggg 1081 gcgggccaga tttcagcatg tagtgcttgc gttggtactc acgcctgtta tactatgagt 1141 actcacgcac agaagggggt tttatggaat acgaaaaaag cgcttcaggg tcggtctacc 1201 tgatcaaaag tgacaagggc tattggttgc ccggtggctt tggttatacg tcaaacaagg 1261 ccgaggctgg ccgcttttca gtcgctgata tggccagcct taaccttgac ggctgcacct 1321 tgtccttgtt ccgcgaagac aagcctttcg gccccggcaa gtttctcggt gactgatatg 1381 aaagaccaaa aggacaagca gaccggcgac ctgctggcca gccctgacgc tgtacgccaa 1441 gcgcgatatg ccgagcgcat gaaggccaaa gggatgcgtc agcgcaagtt ctggctgacc 1501 gacgacgaat acgaggcgct gcgcgagtgc ctggaagaac tcagagcggc gcagggcggg 1561 ggtagtgacc ccgccagcgc ctaaccacca actgcctgca aaggaggcaa tcaatggcta 1621 cccataagcc tatcaatatt ctggaggcgt tcgcagcagc gccgccaccg ctggactacg 1681 ttttgcccaa catggtggcc ggtacggtcg gggcgctggt gtcgcccggt ggtgccggta 1741 aatccatgct ggccctgcaa ctggccgcac agattgcagg cgggccggat ctgctggagg 1801 tgggcgaact gcccaccggc ccggtgatct acctgcccgc cgaagacccg cccaccgcca 1861 ttcatcaccg cctgcacgcc cttggggcgc acctcagcgc cgaggaacgg caagccgtgg 1921 ctgacggcct gctgatccag ccgctgatcg gcagcctgcc caacatcatg gccccggagt 1981 ggttcgacgg cctcaagcgc gccgccgagg gccgccgcct gatggtgctg gacacgctgc 2041 gccggttcca catcgaggaa gaaaacgcca gcggccccat ggcccaggtc atcggtcgca 2101 tggaggccat cgccgccgat accgggtgct ctatcgtgtt cctgcaccat gccagcaagg 2161 gcgcggccat gatgggcgca ggcgaccagc agcaggccag ccggggcagc tcggtactgg 2221 tcgataacat ccgctggcag tcctacctgt cgagcatgac cagcgccgag gccgaggaat 2281 ggggtgtgga cgacgaccag cgccggttct tcgtccgctt cggtgtgagc aaggccaact 2341 atggcgcacc gttcgctgat cggtggttca ggcggcatga cggcggggtg ctcaagcccg 2401 ccgtgctgga gaggcagcgc aagagcaagg gggtgccccg tggtgaagcc taagaacaag 2461 cacagcctca gccacgtccg gcacgacccg gcgcactgtc tggcccccgg cctgttccgt 2521 gccctcaagc ggggcgagcg caagcgcagc aagctggacg tgacgtatga ctacggcgac 2581 ggcaagcgga tcgagttcag cggcccggag ccgctgggcg ctgatgatct gcgcatcctg 2641 caagggctgg tggccatggc tgggcctaat ggcctagtgc ttggcccgga acccaagacc 2701 gaaggcggac ggcagctccg gctgttcctg gaacccaagt gggaggccgt caccgctgaa 2761 tgccatgtgg tcaaaggtag ctatcgggcg ctggcaaagg aaatcggggc agaggtcgat 2821 agtggtgggg cgctcaagca catacaggac tgcatcgagc gcctttggaa ggtatccatc 2881 atcgcccaga atggccgcaa gcggcagggg tttcggctgc tgtcggagta cgccagcgac 2941 gaggcggacg ggcgcctgta cgtggccctg aaccccttga tcgcgcaggc cgtcatgggt 3001 ggcggccagc atgtgcgcat cagcatggac gaggtgcggg cgctggacag cgaaaccgcc 3061 cgcctgctgc accagcggct gtgtggctgg atcgaccccg gcaaaaccgg caaggcttcc 3121 atagatacct tgtgcggcta tgtctggccg tcagaggcca gtggttcgac catgcgcaag 3181 cgccgcaagc gggtgcgcga ggcgttgccg gagctggtcg cgctgggctg gacggtaacc 3241 gagttcgcgg cgggcaagta cgacatcacc cggcccaagg cggcaggctg acccccccca 3301 ctctattgta aacaagacat ttttatcttt tatattcaat ggcttatttt cctgctaatt 3361 ggtaatacca tgaaaaatac catgctcaga aaaggcttaa caatattttg aaaaattgcc 3421 tactgagcgc tgccgcacag ctccataggc tactagagaa cccctgcaat aactgtcacg 3481 cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca aacctgcaaa 3541 cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt atctaagaat 3601 aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg cccaatatgc 3661 ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc gccccaccgc 3721 tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac cccgcagaaa 3781 tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag ggtgggggcg 3841 cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gctactagag tggtgcaaaa 3901 cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac tagagtggca 3961 gcaaaacaac tatggaaaat tttcaatcct agaccgatga agggtggata ctagagcgta 4021 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 4081 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 4141 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 4201 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact 4261 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg 4321 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa 4381 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat 4441 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag 4501 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc 4561 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca 4621 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt 4681 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taattactag 4741 aggaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc 4801 tgcccggctg acgccgttgg atacaccaag gaaagtctac a //