##gff-version 3
##sequence-region BBa_K125020 1 4841
BBa_K125020	.	tag	3956	4008	.	+	0	ID=BBa_K125310;Name=BBa_K125310
BBa_K125020	.	sequence_feature	3956	4008	.	+	0	ID=annotation1968259;Name=slr2016 signal sequence;Parent=BBa_K125310
BBa_K125020	.	promoter	3891	3927	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_K125020	.	promoter	3916	3921	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K125020	.	promoter	3893	3898	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K125020	.	operator	3891	3927	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K125020	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_K125020	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K125020	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K125020	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K125020	.	ribosome_entry_site	3936	3947	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K125020	.	conserved_region	3940	3943	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K125020	.	sequence_feature	3459	3882	.	+	0	ID=BBa_K125340;Name=BBa_K125340
BBa_K125020	.	non_covalent_binding_site	3811	3859	.	+	0	ID=annotation1974216;Name=RepA Binding Site;Parent=BBa_K125340
BBa_K125020	.	non_covalent_binding_site	3461	3524	.	+	0	ID=annotation1974214;Name=RepC Binding Site;Parent=BBa_K125340
BBa_K125020	.	non_covalent_binding_site	3665	3810	.	+	0	ID=annotation1974215;Name=RepB Binding Site;Parent=BBa_K125340
BBa_K125020	.	CDS	66	3450	.	+	0	ID=BBa_K125510;Name=BBa_K125510
BBa_K125020	.	engineered_region	66	102	.	+	0	ID=annotation1974185;Name=I14032;Parent=BBa_K125510
BBa_K125020	.	engineered_region	111	125	.	+	0	ID=annotation1974186;Name=B0030;Parent=BBa_K125510
BBa_K125020	.	CDS	132	1103	.	+	0	ID=annotation1974187;Name=RepB';Parent=BBa_K125510
BBa_K125020	.	promoter	1093	1144	.	+	0	ID=annotation1974188;Name=Promoter[EFRepAC];Parent=BBa_K125510
BBa_K125020	.	CDS	1164	3291	.	+	0	ID=annotation1974189;Name=[EFRepAC];Parent=BBa_K125510
BBa_K125020	.	stem_loop	3418	3450	.	+	0	ID=annotation1974190;Name=Native Terminator;Parent=BBa_K125510
BBa_K125020	.	CDS	4017	4734	.	+	0	ID=BBa_K125500;Name=BBa_K125500
BBa_K125020	.	CDS	4017	4734	.	+	0	ID=annotation1968260;Name=GFP fusion brick;Parent=BBa_K125500
BBa_K125020	.	point_mutation	4017	4017	.	+	0	ID=annotation1969908;Name=deletion of TG;Parent=BBa_K125500
BBa_K125020	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K125020	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K125020	.	oriT	4743	4841	.	+	0	ID=BBa_K125320;Name=BBa_K125320
>BBa_K125020
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagagtggtgcaaaacctt
tcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgaagaacgacaggactttgcaggcc
ataggccgacagctcaaggccatgggctgtgagcgcttcgatatcggcgtcagggacgcacccaccggccagatgatga
accgggaatggtcagccgccgaagtgctccagaacacgccatggctcaagcggatgaatgcccagggcaatgacgtgta
tatcaggcccgccgagcaggagcggcatggtctggtgctggtggacgacctcagcgagtttgacctggatgacatgaaa
gccgagggccgggagcctgccctggtagtggaaaccagcccgaagaactatcaggcatgggtcaaggtggccgacgccg
caggcggtgaacttcgggggcagattgcccggacgctggccagcgagtacgacgccgacccggccagcgccgacagccg
ccactatggccgcttggcgggcttcaccaaccgcaaggacaagcacaccacccgcgccggttatcagccgtgggtgctg
ctgcgtgaatccaagggcaagaccgccaccgctggcccggcgctggtgcagcaggctggccagcagatcgagcaggccc
agcggcagcaggagaaggcccgcaggctggccagcctcgaactgcccgagcggcagcttagccgccaccggcgcacggc
gctggacgagtaccgcagcgagatggccgggctggtcaagcgcttcggtcatgacctcagcaagtgcgactttatcgcc
gcgcagaagctggccagccggggccgcagtgccgaggaaatcggcaaggccatggccgaggccagcccagcgctggcag
agcgcaagcccggccacgaagcggattacatcgagcgcaccgtcagcaaggtcatgggtctgcccagcgtccagcttgc
gcgggccgagctggcacgggcaccggcaccccgccagcgaggcatggacaggggcgggccagatttcagcatgtagtgc
ttgcgttggtactcacgcctgttatactatgagtactcacgcacagaagggggttttatggaatacgaaaaaagcgctt
cagggtcggtctacctgatcaaaagtgacaagggctattggttgcccggtggctttggttatacgtcaaacaaggccga
ggctggccgcttttcagtcgctgatatggccagccttaaccttgacggctgcaccttgtccttgttccgcgaagacaag
cctttcggccccggcaagtttctcggtgactgatatgaaagaccaaaaggacaagcagaccggcgacctgctggccagc
cctgacgctgtacgccaagcgcgatatgccgagcgcatgaaggccaaagggatgcgtcagcgcaagttctggctgaccg
acgacgaatacgaggcgctgcgcgagtgcctggaagaactcagagcggcgcagggcgggggtagtgaccccgccagcgc
ctaaccaccaactgcctgcaaaggaggcaatcaatggctacccataagcctatcaatattctggaggcgttcgcagcag
cgccgccaccgctggactacgttttgcccaacatggtggccggtacggtcggggcgctggtgtcgcccggtggtgccgg
taaatccatgctggccctgcaactggccgcacagattgcaggcgggccggatctgctggaggtgggcgaactgcccacc
ggcccggtgatctacctgcccgccgaagacccgcccaccgccattcatcaccgcctgcacgcccttggggcgcacctca
gcgccgaggaacggcaagccgtggctgacggcctgctgatccagccgctgatcggcagcctgcccaacatcatggcccc
ggagtggttcgacggcctcaagcgcgccgccgagggccgccgcctgatggtgctggacacgctgcgccggttccacatc
gaggaagaaaacgccagcggccccatggcccaggtcatcggtcgcatggaggccatcgccgccgataccgggtgctcta
tcgtgttcctgcaccatgccagcaagggcgcggccatgatgggcgcaggcgaccagcagcaggccagccggggcagctc
ggtactggtcgataacatccgctggcagtcctacctgtcgagcatgaccagcgccgaggccgaggaatggggtgtggac
gacgaccagcgccggttcttcgtccgcttcggtgtgagcaaggccaactatggcgcaccgttcgctgatcggtggttca
ggcggcatgacggcggggtgctcaagcccgccgtgctggagaggcagcgcaagagcaagggggtgccccgtggtgaagc
ctaagaacaagcacagcctcagccacgtccggcacgacccggcgcactgtctggcccccggcctgttccgtgccctcaa
gcggggcgagcgcaagcgcagcaagctggacgtgacgtatgactacggcgacggcaagcggatcgagttcagcggcccg
gagccgctgggcgctgatgatctgcgcatcctgcaagggctggtggccatggctgggcctaatggcctagtgcttggcc
cggaacccaagaccgaaggcggacggcagctccggctgttcctggaacccaagtgggaggccgtcaccgctgaatgcca
tgtggtcaaaggtagctatcgggcgctggcaaaggaaatcggggcagaggtcgatagtggtggggcgctcaagcacata
caggactgcatcgagcgcctttggaaggtatccatcatcgcccagaatggccgcaagcggcaggggtttcggctgctgt
cggagtacgccagcgacgaggcggacgggcgcctgtacgtggccctgaaccccttgatcgcgcaggccgtcatgggtgg
cggccagcatgtgcgcatcagcatggacgaggtgcgggcgctggacagcgaaaccgcccgcctgctgcaccagcggctg
tgtggctggatcgaccccggcaaaaccggcaaggcttccatagataccttgtgcggctatgtctggccgtcagaggcca
gtggttcgaccatgcgcaagcgccgcaagcgggtgcgcgaggcgttgccggagctggtcgcgctgggctggacggtaac
cgagttcgcggcgggcaagtacgacatcacccggcccaaggcggcaggctgaccccccccactctattgtaaacaagac
atttttatcttttatattcaatggcttattttcctgctaattggtaataccatgaaaaataccatgctcagaaaaggct
taacaatattttgaaaaattgcctactgagcgctgccgcacagctccataggctactagagaacccctgcaataactgt
cacgcccccctgcaataactgtcacgaacccctgcaataactgtcacgcccccaaacctgcaaacccagcaggggcggg
ggctggcggggtgttggaaaaatccatccatgattatctaagaataatccactaggcgcggttatcagcgcccttgtgg
ggcgctgctgcccttgcccaatatgcccggccagaggccggatagctggtctattcgctgcgctaggctacacaccgcc
ccaccgctgcgcggcagggggaaaggcgggcaaagcccgctaaaccccacaccaaaccccgcagaaatacgctggagcg
cttttagccgctttagcggcctttccccctacccgaagggtgggggcgcgtgtgcagccccgcagggcctgtctcggtc
gatcattcagctactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatac
tagagtggcagcaaaacaactatggaaaattttcaatcctagaccgatgaagggtggatactagagcgtaaaggagaag
aacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggaga
gggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggcca
acacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttca
agagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctga
agtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattctt
ggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagtta
acttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcga
tggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagac
cacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataattactag
aggaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttg
gatacaccaaggaaagtctaca