lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT
This part was derived from RSF1010 and RP4.
This is an intermediate construction step for the construction of the broad-host-range plasmid derived from RSF1010. The coding region was inserted into pSB1A3.
I am having trouble inserting this part into the Base Vector so the construct will be tested while still in pSB1A3.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Constitutive Promoter, High Transcription
Vikram Vijayan, Allen Hsu, Lawrence Fomundam
slr2016 signal sequence from cyanobacterium Synechocystis; secretes protein
The ''slr2016'' signal sequence is located at the amino terminus of slr2016 polypeptides synthesized by ''Synechocystis'' sp. PCC 6803.
The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
The first two nucleotides of the GFP start codon were removed to create this fusion brick.
OriT-RP4 (Origin of transfer for the RP4 plasmid)
Relaxation region of self-transmissible plasmid RP4. When this part is added to a plasmid, it is transferred by RP4 through conjugation.
This part is similar to J01003, which has no experience and is much longer. In the process of designing and testing this part, it will be compared to J01003.
This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.
This part is to be constructed with the Rep proteins derived from an RSF1010 plasmid.
This part will be constructed with the origin of vegetative replication derived from an RSF1010 plasmid.
Conversion of the iGEM parts registry to SBOL2.1