B-H-R plas
type: ComponentDefinition
persistent identity: BBa K125020
display id: BBa_K125020
version: 1
was generated by: iGEM to SBOL conversion
description: lac promoter/rbs/rep/oriV/lac promoter/rbs/aadA/oriT
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: This part was derived from RSF1010 and RP4.
owned by: james
owned by: myers
mutable description: This is an intermediate construction step for the construction of the broad-host-range plasmid derived from RSF1010. The coding region was inserted into pSB1A3.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: I am having trouble inserting this part into the Base Vector so the construct will be tested while still in pSB1A3.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000110
role: partType/Temporary
component: BBa_I14032
component: BBa_K125310
component: BBa_B0034
component: BBa_K125340
component: BBa_K125320
component: BBa_K125500
component: BBa_K125510
sequence annotation: BBa_I14032
sequence annotation: BBa_B0034
sequence annotation: BBa_K125510
sequence annotation: BBa_K125340
sequence annotation: BBa_K125310
sequence annotation: BBa_K125500
sequence annotation: BBa_K125320
sequence: https://synbiohub.org/public/igem/BBa_K125020_sequence/1

BBa_I14032
type: Component
persistent identity: component1992576
display id: component1992576
version: 1
top level: B-H-R plas
definition: P(Lac) IQ
access: public

BBa_K125310
type: Component
persistent identity: component1992597
display id: component1992597
version: 1
top level: B-H-R plas
definition: slr2016 SS
access: public

BBa_B0034
type: Component
persistent identity: component1992595
display id: component1992595
version: 1
top level: B-H-R plas
definition: BBa_B0034
access: public

BBa_I14032
type: Component
persistent identity: component1992593
display id: component1992593
version: 1
top level: B-H-R plas
definition: P(Lac) IQ
access: public

BBa_K125340
type: Component
persistent identity: component1992589
display id: component1992589
version: 1
top level: B-H-R plas
definition: OriV
access: public

BBa_K125320
type: Component
persistent identity: component1992600
display id: component1992600
version: 1
top level: B-H-R plas
definition: OriT-RP4
access: public

BBa_K125500
type: Component
persistent identity: component1992599
display id: component1992599
version: 1
top level: B-H-R plas
definition: BBa_K125500
access: public

BBa_K125510
type: Component
persistent identity: component1992585
display id: component1992585
version: 1
top level: B-H-R plas
definition: Rep
access: public

BBa_B0034
type: Component
persistent identity: component1992578
display id: component1992578
version: 1
top level: B-H-R plas
definition: BBa_B0034
access: public

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992576
display id: annotation1992576
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992576/range1992576/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125020/annotation1992576/range1992576/1
type: Range
persistent identity: range1992576
display id: range1992576
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 1
end: 37
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1992578
display id: annotation1992578
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992578/range1992578/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125020/annotation1992578/range1992578/1
type: Range
persistent identity: range1992578
display id: range1992578
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 46
end: 57
orientation: inline

BBa_K125510
type: SequenceAnnotation
persistent identity: annotation1992585
display id: annotation1992585
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992585/range1992585/1
component: BBa_K125510

https://synbiohub.org/public/igem/BBa_K125020/annotation1992585/range1992585/1
type: Range
persistent identity: range1992585
display id: range1992585
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 66
end: 3450
orientation: inline

BBa_K125340
type: SequenceAnnotation
persistent identity: annotation1992589
display id: annotation1992589
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992589/range1992589/1
component: BBa_K125340

https://synbiohub.org/public/igem/BBa_K125020/annotation1992589/range1992589/1
type: Range
persistent identity: range1992589
display id: range1992589
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 3459
end: 3882
orientation: inline

BBa_I14032
type: SequenceAnnotation
persistent identity: annotation1992593
display id: annotation1992593
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992593/range1992593/1
component: BBa_I14032

https://synbiohub.org/public/igem/BBa_K125020/annotation1992593/range1992593/1
type: Range
persistent identity: range1992593
display id: range1992593
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 3891
end: 3927
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1992595
display id: annotation1992595
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992595/range1992595/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K125020/annotation1992595/range1992595/1
type: Range
persistent identity: range1992595
display id: range1992595
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 3936
end: 3947
orientation: inline

BBa_K125310
type: SequenceAnnotation
persistent identity: annotation1992597
display id: annotation1992597
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992597/range1992597/1
component: BBa_K125310

https://synbiohub.org/public/igem/BBa_K125020/annotation1992597/range1992597/1
type: Range
persistent identity: range1992597
display id: range1992597
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 3956
end: 4008
orientation: inline

BBa_K125500
type: SequenceAnnotation
persistent identity: annotation1992599
display id: annotation1992599
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992599/range1992599/1
component: BBa_K125500

https://synbiohub.org/public/igem/BBa_K125020/annotation1992599/range1992599/1
type: Range
persistent identity: range1992599
display id: range1992599
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 4017
end: 4734
orientation: inline

BBa_K125320
type: SequenceAnnotation
persistent identity: annotation1992600
display id: annotation1992600
version: 1
top level: B-H-R plas
location: https://synbiohub.org/public/igem/BBa_K125020/annotation1992600/range1992600/1
component: BBa_K125320

https://synbiohub.org/public/igem/BBa_K125020/annotation1992600/range1992600/1
type: Range
persistent identity: range1992600
display id: range1992600
version: 1
top level: B-H-R plas
direction: direction/reverse
start: 4743
end: 4841
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

P(Lac) IQ
type: ComponentDefinition
persistent identity: BBa I14032
display id: BBa_I14032
version: 1
was generated by: iGEM to SBOL conversion
description: promoter P(Lac) IQ
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pMAL-p2X
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, High Transcription
discontinued: false
dominant: true
experience: experience/Works
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Lac) IQ
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14032_sequence/1

P(Lac) IQ
type: SequenceAnnotation
persistent identity: annotation1028342
display id: annotation1028342
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14032/annotation1028342/range1028342/1
type: Range
persistent identity: range1028342
display id: range1028342
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 1
end: 37
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1028343
display id: annotation1028343
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028343/range1028343/1
type: Range
persistent identity: range1028343
display id: range1028343
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 3
end: 8
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1028344
display id: annotation1028344
version: 1
top level: P(Lac) IQ
location: https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I14032/annotation1028344/range1028344/1
type: Range
persistent identity: range1028344
display id: range1028344
version: 1
top level: P(Lac) IQ
direction: direction/forward
start: 26
end: 31
orientation: inline

slr2016 SS
type: ComponentDefinition
persistent identity: BBa K125310
display id: BBa_K125310
version: 1
was generated by: iGEM to SBOL conversion
description: slr2016 signal sequence from cyanobacterium Synechocystis; secretes protein
created: 2008-07-24T11:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: The ''slr2016'' signal sequence is located at the amino terminus of slr2016 polypeptides synthesized by ''Synechocystis'' sp. PCC 6803.
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Krystle Salazar and Grace Kwan
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: slr2016 signal sequence
sequence: https://synbiohub.org/public/igem/BBa_K125310_sequence/1

slr2016 signal sequence
type: SequenceAnnotation
persistent identity: annotation1968259
display id: annotation1968259
version: 1
top level: slr2016 SS
location: https://synbiohub.org/public/igem/BBa_K125310/annotation1968259/range1968259/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K125310/annotation1968259/range1968259/1
type: Range
persistent identity: range1968259
display id: range1968259
version: 1
top level: slr2016 SS
direction: direction/forward
start: 1
end: 53
orientation: inline

BBa_K125500
type: ComponentDefinition
persistent identity: BBa K125500
display id: BBa_K125500
version: 1
was generated by: iGEM to SBOL conversion
description: GFP fusion brick
created: 2008-07-24T11:00:00Z
modified: 2016-01-25T02:34:56Z
mutable provenance: The GFP fusion brick was derived from the GFP BioBrick, [http://partsregistry.org/Part:BBa_E0040 BBa_E0040].
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 4206
owner id: 3229
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The first two nucleotides of the GFP start codon were removed to create this fusion brick.
star: false
creator: Grace Kwan
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP fusion brick
sequence annotation: deletion of TG
sequence: https://synbiohub.org/public/igem/BBa_K125500_sequence/1

GFP fusion brick
type: SequenceAnnotation
persistent identity: annotation1968260
display id: annotation1968260
version: 1
top level: BBa_K125500
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K125500/annotation1968260/range1968260/1
type: Range
persistent identity: range1968260
display id: range1968260
version: 1
top level: BBa_K125500
direction: direction/forward
start: 1
end: 718
orientation: inline

deletion of TG
type: SequenceAnnotation
persistent identity: annotation1969908
display id: annotation1969908
version: 1
top level: BBa_K125500
location: https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K125500/annotation1969908/range1969908/1
type: Range
persistent identity: range1969908
display id: range1969908
version: 1
top level: BBa_K125500
direction: direction/forward
start: 1
end: 1
orientation: inline

OriT-RP4
type: ComponentDefinition
persistent identity: BBa K125320
display id: BBa_K125320
version: 1
was generated by: iGEM to SBOL conversion
description: OriT-RP4 (Origin of transfer for the RP4 plasmid)
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: RP4 NCBI accession number NC_001621
owned by: james
owned by: myers
mutable description: Relaxation region of self-transmissible plasmid RP4. When this part is added to a plasmid, it is transferred by RP4 through conjugation.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part is similar to J01003, which has no experience and is much longer. In the process of designing and testing this part, it will be compared to J01003.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000724
role: partType/Conjugation
sequence: https://synbiohub.org/public/igem/BBa_K125320_sequence/1

OriV
type: ComponentDefinition
persistent identity: BBa K125340
display id: BBa_K125340
version: 1
was generated by: iGEM to SBOL conversion
description: Origin of Vegetative Replication
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
owned by: james
owned by: myers
mutable description: Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part is to be constructed with the Rep proteins derived from an RSF1010 plasmid.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: RepC Binding Site
sequence annotation: RepB Binding Site
sequence annotation: RepA Binding Site
sequence: https://synbiohub.org/public/igem/BBa_K125340_sequence/1

RepC Binding Site
type: SequenceAnnotation
persistent identity: annotation1974214
display id: annotation1974214
version: 1
top level: OriV
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974214/range1974214/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K125340/annotation1974214/range1974214/1
type: Range
persistent identity: range1974214
display id: range1974214
version: 1
top level: OriV
direction: direction/forward
start: 3
end: 66
orientation: inline

RepB Binding Site
type: SequenceAnnotation
persistent identity: annotation1974215
display id: annotation1974215
version: 1
top level: OriV
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974215/range1974215/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K125340/annotation1974215/range1974215/1
type: Range
persistent identity: range1974215
display id: range1974215
version: 1
top level: OriV
direction: direction/forward
start: 207
end: 352
orientation: inline

RepA Binding Site
type: SequenceAnnotation
persistent identity: annotation1974216
display id: annotation1974216
version: 1
top level: OriV
location: https://synbiohub.org/public/igem/BBa_K125340/annotation1974216/range1974216/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K125340/annotation1974216/range1974216/1
type: Range
persistent identity: range1974216
display id: range1974216
version: 1
top level: OriV
direction: direction/forward
start: 353
end: 401
orientation: inline

Rep
type: ComponentDefinition
persistent identity: BBa K125510
display id: BBa_K125510
version: 1
was generated by: iGEM to SBOL conversion
description: Replication Proteins
created: 2008-08-28T11:00:00Z
modified: 2015-05-08T01:09:45Z
mutable provenance: This part is taken from the RSF1010 derived plasmid pRL1383a. A description of this plasmid can be found at the NCBI database at accession number AF403426.
owned by: james
owned by: myers
mutable description: Plasmids containing oriV and the corresponding Rep proteins (from RSF1010) are autonomously replicated in a broad range of hosts. It has been reported that RSF1010 derived plasmids containing oriV and the Rep proteins are stably maintained in Pseudomonas (Bagdasarian 1981), Caulobacter (Umelo-Njaka et al. 2001), Erwinia, Serratia (Leemans 1987), and several cyanobacteria strains (Mermet-Bouvier 1993) including Synechocystis PCC6803 and PCC6714 and Synechococcus PCC7942 and PCC6301.
discontinued: false
dominant: false
experience: experience/None
group u list: _179_
m user id: 0
owner id: 3138
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part will be constructed with the origin of vegetative replication derived from an RSF1010 plasmid.
star: false
creator: Margaret Ruzicka
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: I14032
sequence annotation: B0030
sequence annotation: RepB'
sequence annotation: Promoter[EFRepAC]
sequence annotation: [EFRepAC]
sequence annotation: Native Terminator
sequence: https://synbiohub.org/public/igem/BBa_K125510_sequence/1

I14032
type: SequenceAnnotation
persistent identity: annotation1974185
display id: annotation1974185
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974185/range1974185/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K125510/annotation1974185/range1974185/1
type: Range
persistent identity: range1974185
display id: range1974185
version: 1
top level: Rep
direction: direction/forward
start: 1
end: 37
orientation: inline

B0030
type: SequenceAnnotation
persistent identity: annotation1974186
display id: annotation1974186
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974186/range1974186/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K125510/annotation1974186/range1974186/1
type: Range
persistent identity: range1974186
display id: range1974186
version: 1
top level: Rep
direction: direction/forward
start: 46
end: 60
orientation: inline

RepB'
type: SequenceAnnotation
persistent identity: annotation1974187
display id: annotation1974187
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974187/range1974187/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K125510/annotation1974187/range1974187/1
type: Range
persistent identity: range1974187
display id: range1974187
version: 1
top level: Rep
direction: direction/forward
start: 67
end: 1038
orientation: inline

Promoter[EFRepAC]
type: SequenceAnnotation
persistent identity: annotation1974188
display id: annotation1974188
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974188/range1974188/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K125510/annotation1974188/range1974188/1
type: Range
persistent identity: range1974188
display id: range1974188
version: 1
top level: Rep
direction: direction/forward
start: 1028
end: 1079
orientation: inline

[EFRepAC]
type: SequenceAnnotation
persistent identity: annotation1974189
display id: annotation1974189
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974189/range1974189/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K125510/annotation1974189/range1974189/1
type: Range
persistent identity: range1974189
display id: range1974189
version: 1
top level: Rep
direction: direction/forward
start: 1099
end: 3226
orientation: inline

Native Terminator
type: SequenceAnnotation
persistent identity: annotation1974190
display id: annotation1974190
version: 1
top level: Rep
location: https://synbiohub.org/public/igem/BBa_K125510/annotation1974190/range1974190/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K125510/annotation1974190/range1974190/1
type: Range
persistent identity: range1974190
display id: range1974190
version: 1
top level: Rep
direction: direction/forward
start: 3353
end: 3385
orientation: inline

https://synbiohub.org/public/igem/BBa_K125020_sequence/1
type: Sequence
persistent identity: BBa K125020 sequence
display id: BBa_K125020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagagtggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgaagaacgacaggactttgcaggccataggccgacagctcaaggccatgggctgtgagcgcttcgatatcggcgtcagggacgcacccaccggccagatgatgaaccgggaatggtcagccgccgaagtgctccagaacacgccatggctcaagcggatgaatgcccagggcaatgacgtgtatatcaggcccgccgagcaggagcggcatggtctggtgctggtggacgacctcagcgagtttgacctggatgacatgaaagccgagggccgggagcctgccctggtagtggaaaccagcccgaagaactatcaggcatgggtcaaggtggccgacgccgcaggcggtgaacttcgggggcagattgcccggacgctggccagcgagtacgacgccgacccggccagcgccgacagccgccactatggccgcttggcgggcttcaccaaccgcaaggacaagcacaccacccgcgccggttatcagccgtgggtgctgctgcgtgaatccaagggcaagaccgccaccgctggcccggcgctggtgcagcaggctggccagcagatcgagcaggcccagcggcagcaggagaaggcccgcaggctggccagcctcgaactgcccgagcggcagcttagccgccaccggcgcacggcgctggacgagtaccgcagcgagatggccgggctggtcaagcgcttcggtcatgacctcagcaagtgcgactttatcgccgcgcagaagctggccagccggggccgcagtgccgaggaaatcggcaaggccatggccgaggccagcccagcgctggcagagcgcaagcccggccacgaagcggattacatcgagcgcaccgtcagcaaggtcatgggtctgcccagcgtccagcttgcgcgggccgagctggcacgggcaccggcaccccgccagcgaggcatggacaggggcgggccagatttcagcatgtagtgcttgcgttggtactcacgcctgttatactatgagtactcacgcacagaagggggttttatggaatacgaaaaaagcgcttcagggtcggtctacctgatcaaaagtgacaagggctattggttgcccggtggctttggttatacgtcaaacaaggccgaggctggccgcttttcagtcgctgatatggccagccttaaccttgacggctgcaccttgtccttgttccgcgaagacaagcctttcggccccggcaagtttctcggtgactgatatgaaagaccaaaaggacaagcagaccggcgacctgctggccagccctgacgctgtacgccaagcgcgatatgccgagcgcatgaaggccaaagggatgcgtcagcgcaagttctggctgaccgacgacgaatacgaggcgctgcgcgagtgcctggaagaactcagagcggcgcagggcgggggtagtgaccccgccagcgcctaaccaccaactgcctgcaaaggaggcaatcaatggctacccataagcctatcaatattctggaggcgttcgcagcagcgccgccaccgctggactacgttttgcccaacatggtggccggtacggtcggggcgctggtgtcgcccggtggtgccggtaaatccatgctggccctgcaactggccgcacagattgcaggcgggccggatctgctggaggtgggcgaactgcccaccggcccggtgatctacctgcccgccgaagacccgcccaccgccattcatcaccgcctgcacgcccttggggcgcacctcagcgccgaggaacggcaagccgtggctgacggcctgctgatccagccgctgatcggcagcctgcccaacatcatggccccggagtggttcgacggcctcaagcgc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14032_sequence/1
type: Sequence
persistent identity: BBa I14032 sequence
display id: BBa_I14032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125310_sequence/1
type: Sequence
persistent identity: BBa K125310 sequence
display id: BBa_K125310_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggcagcaaaacaactatggaaaattttcaatcctagaccgatgaagggtgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125500_sequence/1
type: Sequence
persistent identity: BBa K125500 sequence
display id: BBa_K125500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125320_sequence/1
type: Sequence
persistent identity: BBa K125320 sequence
display id: BBa_K125320_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaataagggacagtgaagaaggaacacccgctcgcgggtgggcctacttcacctatcctgcccggctgacgccgttggatacaccaaggaaagtctaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125340_sequence/1
type: Sequence
persistent identity: BBa K125340 sequence
display id: BBa_K125340_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacccctgcaataactgtcacgcccccctgcaataactgtcacgaacccctgcaataactgtcacgcccccaaacctgcaaacccagcaggggcgggggctggcggggtgttggaaaaatccatccatgattatctaagaataatccactaggcgcggttatcagcgcccttgtggggcgctgctgcccttgcccaatatgcccggccagaggccggatagctggtctattcgctgcgctaggctacacaccgccccaccgctgcgcggcagggggaaaggcgggcaaagcccgctaaaccccacaccaaaccccgcagaaatacgctggagcgcttttagccgctttagcggcctttccccctacccgaagggtgggggcgcgtgtgcagccccgcagggcctgtctcggtcgatcattcagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K125510_sequence/1
type: Sequence
persistent identity: BBa K125510 sequence
display id: BBa_K125510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagattaaagaggagaaatactagatgaagaacgacaggactttgcaggccataggccgacagctcaaggccatgggctgtgagcgcttcgatatcggcgtcagggacgcacccaccggccagatgatgaaccgggaatggtcagccgccgaagtgctccagaacacgccatggctcaagcggatgaatgcccagggcaatgacgtgtatatcaggcccgccgagcaggagcggcatggtctggtgctggtggacgacctcagcgagtttgacctggatgacatgaaagccgagggccgggagcctgccctggtagtggaaaccagcccgaagaactatcaggcatgggtcaaggtggccgacgccgcaggcggtgaacttcgggggcagattgcccggacgctggccagcgagtacgacgccgacccggccagcgccgacagccgccactatggccgcttggcgggcttcaccaaccgcaaggacaagcacaccacccgcgccggttatcagccgtgggtgctgctgcgtgaatccaagggcaagaccgccaccgctggcccggcgctggtgcagcaggctggccagcagatcgagcaggcccagcggcagcaggagaaggcccgcaggctggccagcctcgaactgcccgagcggcagcttagccgccaccggcgcacggcgctggacgagtaccgcagcgagatggccgggctggtcaagcgcttcggtcatgacctcagcaagtgcgactttatcgccgcgcagaagctggccagccggggccgcagtgccgaggaaatcggcaaggccatggccgaggccagcccagcgctggcagagcgcaagcccggccacgaagcggattacatcgagcgcaccgtcagcaaggtcatgggtctgcccagcgtccagcttgcgcgggccgagctggcacgggcaccggcaccccgccagcgaggcatggacaggggcgggccagatttcagcatgtagtgcttgcgttggtactcacgcctgttatactatgagtactcacgcacagaagggggttttatggaatacgaaaaaagcgcttcagggtcggtctacctgatcaaaagtgacaagggctattggttgcccggtggctttggttatacgtcaaacaaggccgaggctggccgcttttcagtcgctgatatggccagccttaaccttgacggctgcaccttgtccttgttccgcgaagacaagcctttcggccccggcaagtttctcggtgactgatatgaaagaccaaaaggacaagcagaccggcgacctgctggccagccctgacgctgtacgccaagcgcgatatgccgagcgcatgaaggccaaagggatgcgtcagcgcaagttctggctgaccgacgacgaatacgaggcgctgcgcgagtgcctggaagaactcagagcggcgcagggcgggggtagtgaccccgccagcgcctaaccaccaactgcctgcaaaggaggcaatcaatggctacccataagcctatcaatattctggaggcgttcgcagcagcgccgccaccgctggactacgttttgcccaacatggtggccggtacggtcggggcgctggtgtcgcccggtggtgccggtaaatccatgctggccctgcaactggccgcacagattgcaggcgggccggatctgctggaggtgggcgaactgcccaccggcccggtgatctacctgcccgccgaagacccgcccaccgccattcatcaccgcctgcacgcccttggggcgcacctcagcgccgaggaacggcaagccgtggctgacggcctgctgatccagccgctgatcggcagcctgcccaacatcatggccccggagtggttcgacggcctcaagcgcgccgccgagggccgccgcctgatggtgctggacacgctgcgccggttccacatcgaggaagaaaa…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z