BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K125100
1
BBa_K125100
nir promoter from <I>Synechocystis</I> sp. PCC6803
2008-07-23T11:00:00Z
2015-05-08T01:09:44Z
The ''nirA'' promoter originates from ''Synechocystis'' sp. PCC6803.
The ''nir'' promoter is nitrate inducible.
false
false
_
0
3229
9
It's complicated
false
false
Krystle Salazar and Grace Kwan
annotation1968489
1
Ntc-A binding motif
range1968489
1
36
44
annotation1968488
1
Ntc-B binding motif
range1968488
1
5
20
annotation1968487
1
-10 sequence
range1968487
1
73
78
annotation1968486
1
nir promoter
range1968486
1
1
88
BBa_K125110
1
BBa_K125110
nir promoter + rbs (0.6)
2008-10-27T12:00:00Z
2015-05-08T01:09:44Z
nir was derived from ''Synechocystis'' sp. PCC 6803 and the rbs is that entered in the registry.
The cyanobacterial ''nir'' promoter was ligated to an rbs.
false
false
_179_
0
3229
9
It's complicated
false
n/a
false
Grace Kwan
component1992517
1
BBa_B0030
component1992515
1
BBa_K125100
annotation1992515
1
BBa_K125100
range1992515
1
1
88
annotation1992517
1
BBa_B0030
range1992517
1
97
111
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K125100_sequence
1
gctaaatgcgtaaactgcatatgccttcgctgagtgtaatttacgttacaaattttaacgaaacgggaaccctatattgatctctact
BBa_K125110_sequence
1
gctaaatgcgtaaactgcatatgccttcgctgagtgtaatttacgttacaaattttaacgaaacgggaaccctatattgatctctacttactagagattaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z