##gff-version 3
##sequence-region BBa_K1253113 1 4392
BBa_K1253113	.	engineered_region	2948	4255	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_K1253113	.	ribosome_entry_site	2948	2959	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K1253113	.	conserved_region	2952	2955	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1253113	.	terminator	4215	4255	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K1253113	.	stop_codon	4248	4248	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253113	.	polyA_site	4242	4255	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253113	.	stem_loop	4222	4241	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253113	.	engineered_region	4215	4255	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253113	.	CDS	2966	4093	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K1253113	.	engineered_tag	4094	4118	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1253113	.	engineered_region	2966	4093	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K1253113	.	tag	4055	4093	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1253113	.	CDS	2966	4093	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1253113	.	terminator	4127	4206	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K1253113	.	engineered_region	4127	4206	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253113	.	stem_loop	4138	4181	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253113	.	engineered_region	1843	2720	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1253113	.	ribosome_entry_site	1843	1857	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1253113	.	ribosome_entry_site	1850	1854	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1253113	.	engineered_region	1843	1857	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1253113	.	ribosome_entry_site	1843	1857	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1253113	.	terminator	2680	2720	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1253113	.	stop_codon	2713	2713	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253113	.	polyA_site	2707	2720	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253113	.	stem_loop	2687	2706	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253113	.	engineered_region	2680	2720	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253113	.	CDS	1864	2583	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1253113	.	CDS	1864	2583	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1253113	.	terminator	2592	2671	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1253113	.	engineered_region	2592	2671	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253113	.	stem_loop	2603	2646	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253113	.	engineered_region	84	923	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K1253113	.	terminator	883	923	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1253113	.	stop_codon	916	916	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253113	.	polyA_site	910	923	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253113	.	stem_loop	890	909	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253113	.	engineered_region	883	923	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253113	.	terminator	795	874	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1253113	.	engineered_region	795	874	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253113	.	stem_loop	806	849	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253113	.	CDS	102	761	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1253113	.	engineered_tag	762	786	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1253113	.	CDS	105	721	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1253113	.	tag	722	755	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1253113	.	ribosome_entry_site	84	95	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1253113	.	conserved_region	88	91	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1253113	.	terminator	1644	1772	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1253113	.	terminator	1644	1723	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1253113	.	engineered_region	1644	1723	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253113	.	stem_loop	1655	1698	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253113	.	terminator	1732	1772	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1253113	.	stop_codon	1765	1765	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253113	.	polyA_site	1759	1772	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253113	.	stem_loop	1739	1758	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253113	.	engineered_region	1732	1772	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253113	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1253113	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1253113	.	ribosome_entry_site	2928	2939	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1253113	.	conserved_region	2932	2935	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1253113	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1253113	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1253113	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1253113	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1253113	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1253113	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1253113	.	CDS	930	1635	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K1253113	.	engineered_tag	1611	1635	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1253113	.	CDS	930	1604	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1253113	.	promoter	2866	2919	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1253113	.	engineered_region	2866	2919	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1253113	.	promoter	2885	2890	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1253113	.	promoter	2908	2913	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1253113	.	non_covalent_binding_site	2866	2884	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1253113	.	non_covalent_binding_site	2891	2909	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1253113	.	promoter	1781	1834	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1253113	.	engineered_region	1781	1834	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1253113	.	promoter	1800	1805	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1253113	.	promoter	1823	1828	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1253113	.	non_covalent_binding_site	1781	1799	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1253113	.	non_covalent_binding_site	1806	1824	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1253113	.	terminator	2729	2857	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1253113	.	terminator	2729	2808	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1253113	.	engineered_region	2729	2808	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253113	.	stem_loop	2740	2783	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253113	.	terminator	2817	2857	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1253113	.	stop_codon	2850	2850	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253113	.	polyA_site	2844	2857	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253113	.	stem_loop	2824	2843	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253113	.	engineered_region	2817	2857	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253113	.	terminator	4264	4392	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1253113	.	terminator	4264	4343	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1253113	.	engineered_region	4264	4343	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253113	.	stem_loop	4275	4318	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253113	.	terminator	4352	4392	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1253113	.	stop_codon	4385	4385	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253113	.	polyA_site	4379	4392	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253113	.	stem_loop	4359	4378	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253113	.	engineered_region	4352	4392	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K1253113
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgag
gtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaa
aaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagg
ggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaa
gtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtt
tttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagca
tcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattattt
gatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtg
aaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagatggcttcctccgaagacg
ttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaagg
tgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac
atcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgt
ccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctc
cctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaa
aaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtc
tgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccggg
tgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgct
gaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcactactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagtt
gtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaa
catacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactacttt
cggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaa
ggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtg
atacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaata
caactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacac
aacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttac
cagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttga
gtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactact
agagaaagaggagaaatactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcg
cagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcggga
aaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttg
ctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatc
aactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttct
cgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcact
aatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgc
gactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggc
gcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactgg
agtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacg
atcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggata
cgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaacc
agcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaa
gaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgaca
ggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctag
tgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata