LOCUS BBa_K1253114 4435 bp DNA linear 22-Dec-2024 DEFINITION This part is used to test for TetR half-life. ACCESSION BBa_K1253114 VERSION BBa_K1253114.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 88..91 /label=conserved RBS 84..95 /label=BBa_B0034 CDS 105..721 /label=tetR misc_feature 722..755 /label=SsrA misc_feature 762..786 /label=Help:Barcodes CDS 102..761 /label=BBa_C0040 stem_loop 806..849 /label=stem_loop misc_feature 795..874 /label=BBa_B0010 terminator 795..874 /label=BBa_B0010 stem_loop 890..909 /label=T7 TE misc_feature 916..916 /label=stop polyA_site 910..923 /label=polya misc_feature 883..923 /label=BBa_B0012 terminator 883..923 /label=BBa_B0012 misc_feature 84..923 /label=BBa_P0440 CDS 930..1604 /label=mrfp1 misc_feature 1611..1635 /label=Help:Barcodes CDS 930..1635 /label=BBa_E1010 stem_loop 1655..1698 /label=stem_loop misc_feature 1644..1723 /label=BBa_B0010 terminator 1644..1723 /label=BBa_B0010 stem_loop 1739..1758 /label=T7 TE misc_feature 1765..1765 /label=stop polyA_site 1759..1772 /label=polya misc_feature 1732..1772 /label=BBa_B0012 terminator 1732..1772 /label=BBa_B0012 terminator 1644..1772 /label=BBa_B0015 misc_feature 1781..1799 /label=TetR 1 misc_feature 1781..1834 /label=BBa_R0040 promoter 1800..1805 /label=-35 misc_feature 1806..1824 /label=TetR 2 promoter 1823..1828 /label=-10 promoter 1781..1834 /label=BBa_R0040 RBS 1843..1857 /label=RBS-1\Strong RBS 1850..1854 /label=RBS misc_feature 1843..1857 /label=BBa_B0030 RBS 1843..1857 /label=BBa_B0030 CDS 1864..2583 /label=GFP protein CDS 1864..2583 /label=BBa_E0040 stem_loop 2603..2646 /label=stem_loop misc_feature 2592..2671 /label=BBa_B0010 terminator 2592..2671 /label=BBa_B0010 stem_loop 2687..2706 /label=T7 TE misc_feature 2713..2713 /label=stop polyA_site 2707..2720 /label=polya misc_feature 2680..2720 /label=BBa_B0012 terminator 2680..2720 /label=BBa_B0012 misc_feature 1843..2720 /label=BBa_E0840 stem_loop 2740..2783 /label=stem_loop misc_feature 2729..2808 /label=BBa_B0010 terminator 2729..2808 /label=BBa_B0010 stem_loop 2824..2843 /label=T7 TE misc_feature 2850..2850 /label=stop polyA_site 2844..2857 /label=polya misc_feature 2817..2857 /label=BBa_B0012 terminator 2817..2857 /label=BBa_B0012 terminator 2729..2857 /label=BBa_B0015 misc_feature 2866..2962 /label=BBa_R0065 misc_feature 2871..2890 /label=Lux Box promoter 2912..2917 /label=-10 misc_feature 2922..2938 /label=OR2 cI promoter 2923..2923 /label=lux p(R) start promoter 2936..2941 /label=-35 misc_feature 2946..2962 /label=OR1 cI promoter 2959..2962 /label=-10 promoter 2866..2962 /label=BBa_R0065 misc_feature 2975..2978 /label=conserved RBS 2971..2982 /label=BBa_B0034 misc_feature 2995..2998 /label=conserved RBS 2991..3002 /label=BBa_B0034 misc_feature 4098..4136 /label=LVA CDS 3009..4136 /label=lacI-LVA misc_feature 3009..4136 /label=BBa_C0012 misc_feature 4137..4161 /label=Help:Barcodes CDS 3009..4136 /label=BBa_C0012 stem_loop 4181..4224 /label=stem_loop misc_feature 4170..4249 /label=BBa_B0010 terminator 4170..4249 /label=BBa_B0010 stem_loop 4265..4284 /label=T7 TE misc_feature 4291..4291 /label=stop polyA_site 4285..4298 /label=polya misc_feature 4258..4298 /label=BBa_B0012 terminator 4258..4298 /label=BBa_B0012 misc_feature 2991..4298 /label=BBa_P0412 stem_loop 4318..4361 /label=stem_loop misc_feature 4307..4386 /label=BBa_B0010 terminator 4307..4386 /label=BBa_B0010 stem_loop 4402..4421 /label=T7 TE misc_feature 4428..4428 /label=stop polyA_site 4422..4435 /label=polya misc_feature 4395..4435 /label=BBa_B0012 terminator 4395..4435 /label=BBa_B0012 terminator 4307..4435 /label=BBa_B0015 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gagaaagagg agaaatacta gatgtccaga ttagataaaa 121 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa 181 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa 241 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact 301 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta 361 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag 421 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac 481 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg 541 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc 601 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct 661 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt 721 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata acactgatag tgctagtgta 781 gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 901 gggtgggcct ttctgcgttt atatactaga tggcttcctc cgaagacgtt atcaaagagt 961 tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag 1021 gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag 1081 gtggtccgct gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag 1141 cttacgttaa acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt 1201 tcaaatggga acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact 1261 cctccctgca agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt 1321 ccgacggtcc ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt 1381 acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg 1441 gtcactacga cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg 1501 gtgcttacaa aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg 1561 ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt 1621 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 1681 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 1741 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat 1801 tgacatccct atcagtgata gagatactga gcactactag agattaaaga ggagaaatac 1861 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat 1921 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac 1981 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca 2041 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa 2101 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt 2161 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt 2221 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac 2281 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat 2341 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca 2401 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat 2461 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc 2521 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa 2581 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 2641 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 2701 tgggcctttc tgcgtttata tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2761 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2821 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtaagc acctgtagga 2881 tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataacaccgt gcgtgttgac 2941 tattttacct ctggcggtga tatactagag aaagaggaga aatactagag aaagaggaga 3001 aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 3061 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 3121 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 3181 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 3241 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 3301 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 3361 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 3421 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 3481 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 3541 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 3601 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 3661 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 3721 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 3781 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 3841 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 3901 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 3961 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 4021 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 4081 gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct 4141 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc 4201 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 4261 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagccag gcatcaaata 4321 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 4381 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //