##gff-version 3
##sequence-region BBa_K1253114 1 4435
BBa_K1253114	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K1253114	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K1253114	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K1253114	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K1253114	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K1253114	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K1253114	.	CDS	930	1635	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K1253114	.	CDS	930	1604	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1253114	.	engineered_tag	1611	1635	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1253114	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1253114	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1253114	.	terminator	2729	2857	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1253114	.	terminator	2817	2857	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1253114	.	polyA_site	2844	2857	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253114	.	engineered_region	2817	2857	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253114	.	stem_loop	2824	2843	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253114	.	stop_codon	2850	2850	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253114	.	terminator	2729	2808	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1253114	.	engineered_region	2729	2808	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253114	.	stem_loop	2740	2783	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253114	.	terminator	1644	1772	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1253114	.	terminator	1732	1772	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1253114	.	polyA_site	1759	1772	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253114	.	engineered_region	1732	1772	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253114	.	stem_loop	1739	1758	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253114	.	stop_codon	1765	1765	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253114	.	terminator	1644	1723	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1253114	.	engineered_region	1644	1723	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253114	.	stem_loop	1655	1698	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253114	.	promoter	1781	1834	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K1253114	.	engineered_region	1781	1834	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1253114	.	promoter	1800	1805	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1253114	.	promoter	1823	1828	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1253114	.	non_covalent_binding_site	1781	1799	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1253114	.	non_covalent_binding_site	1806	1824	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1253114	.	promoter	2866	2962	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_K1253114	.	promoter	2959	2962	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K1253114	.	promoter	2936	2941	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K1253114	.	non_covalent_binding_site	2946	2962	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K1253114	.	promoter	2912	2917	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K1253114	.	promoter	2923	2923	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K1253114	.	non_covalent_binding_site	2922	2938	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K1253114	.	non_covalent_binding_site	2871	2890	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K1253114	.	engineered_region	2866	2962	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K1253114	.	terminator	4307	4435	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1253114	.	terminator	4395	4435	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1253114	.	polyA_site	4422	4435	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253114	.	engineered_region	4395	4435	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253114	.	stem_loop	4402	4421	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253114	.	stop_codon	4428	4428	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253114	.	terminator	4307	4386	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1253114	.	engineered_region	4307	4386	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253114	.	stem_loop	4318	4361	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253114	.	engineered_region	84	923	.	+	0	ID=BBa_P0440;Name=BBa_P0440
BBa_K1253114	.	ribosome_entry_site	84	95	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_K1253114	.	conserved_region	88	91	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1253114	.	terminator	883	923	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_K1253114	.	polyA_site	910	923	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253114	.	engineered_region	883	923	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253114	.	stem_loop	890	909	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253114	.	stop_codon	916	916	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253114	.	terminator	795	874	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_K1253114	.	engineered_region	795	874	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253114	.	stem_loop	806	849	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253114	.	CDS	102	761	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_K1253114	.	CDS	105	721	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K1253114	.	tag	722	755	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K1253114	.	engineered_tag	762	786	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K1253114	.	engineered_region	1843	2720	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1253114	.	terminator	2592	2671	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1253114	.	engineered_region	2592	2671	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253114	.	stem_loop	2603	2646	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253114	.	CDS	1864	2583	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1253114	.	CDS	1864	2583	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1253114	.	ribosome_entry_site	1843	1857	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1253114	.	ribosome_entry_site	1843	1857	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1253114	.	engineered_region	1843	1857	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1253114	.	ribosome_entry_site	1850	1854	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1253114	.	terminator	2680	2720	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1253114	.	polyA_site	2707	2720	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253114	.	engineered_region	2680	2720	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253114	.	stem_loop	2687	2706	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253114	.	stop_codon	2713	2713	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253114	.	ribosome_entry_site	2971	2982	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1253114	.	conserved_region	2975	2978	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1253114	.	engineered_region	2991	4298	.	+	0	ID=BBa_P0412;Name=BBa_P0412
BBa_K1253114	.	terminator	4258	4298	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0412
BBa_K1253114	.	polyA_site	4285	4298	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1253114	.	engineered_region	4258	4298	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1253114	.	stem_loop	4265	4284	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1253114	.	stop_codon	4291	4291	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1253114	.	terminator	4170	4249	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0412
BBa_K1253114	.	engineered_region	4170	4249	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1253114	.	stem_loop	4181	4224	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1253114	.	ribosome_entry_site	2991	3002	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0412
BBa_K1253114	.	conserved_region	2995	2998	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1253114	.	CDS	3009	4136	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0412
BBa_K1253114	.	CDS	3009	4136	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K1253114	.	engineered_tag	4137	4161	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K1253114	.	tag	4098	4136	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K1253114	.	engineered_region	3009	4136	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
>BBa_K1253114
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgag
gtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaa
aaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagg
ggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaa
gtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtt
tttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagca
tcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattattt
gatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtg
aaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagatggcttcctccgaagacg
ttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaagg
tgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac
atcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgt
ccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctc
cctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaa
aaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtc
tgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccggg
tgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgct
gaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtg
atagagatactgagcactactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagtt
gtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaa
catacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactacttt
cggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaa
ggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtg
atacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaata
caactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacac
aacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttac
cagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttga
gtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaata
acaccgtgcgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagagaaagaggagaaa
tactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttccc
gcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacat
tcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcac
gcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaac
gaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatcc
gctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccag
acacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcacc
agcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcac
tcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatg
ctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccg
agtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgcc
gttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccag
gcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcct
ctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacga
cgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggccttt
ctgcgtttata