LOCUS       BBa_K125350              979 bp    DNA     linear       22-Dec-2024
DEFINITION  oriTr  with lac promoter and lacZ
ACCESSION   BBa_K125350
VERSION     BBa_K125350.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     oriT            609..979
                     /label=BBa_J01003
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagaggc cgccttttcc tcaatcgctc ttcgttcgtc tggaaggcag tacaccttga
      661 taggtgggct gcccttcctg gttggcttgg tttcatcagc catccgcttg ccctcatctg
      721 ttacgccggc ggtagccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
      781 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
      841 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
      901 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
      961 agggttatgc agcggaaaa
//