LOCUS       BBa_K1253801            1157 bp    DNA     linear       26-Nov-2024
DEFINITION  Survivin Promoter + GFP
ACCESSION   BBa_K1253801
VERSION     BBa_K1253801.1  
FEATURES             Location/Qualifiers
     promoter        1..271
                     /label=BBa_K1253800
     RBS             280..294
                     /label=RBS-1\Strong
     RBS             287..291
                     /label=RBS
     misc_feature    280..294
                     /label=BBa_B0030
     RBS             280..294
                     /label=BBa_B0030
     CDS             301..1020
                     /label=GFP protein
     CDS             301..1020
                     /label=BBa_E0040
     stem_loop       1040..1083
                     /label=stem_loop
     misc_feature    1029..1108
                     /label=BBa_B0010
     terminator      1029..1108
                     /label=BBa_B0010
     stem_loop       1124..1143
                     /label=T7 TE
     misc_feature    1150..1150
                     /label=stop
     polyA_site      1144..1157
                     /label=polya
     misc_feature    1117..1157
                     /label=BBa_B0012
     terminator      1117..1157
                     /label=BBa_B0012
     misc_feature    280..1157
                     /label=BBa_E0840
ORIGIN
        1 cgcgttcttt gaaagcagtc gagggggcgc taggtgtggg cagggacgag ctggcgcggc
       61 gtcgctgggt gcaccgcgac cacgggcaga gccacgcggc gggaggacta caactcccgg
      121 cacaccccgc gccgccccgc ctctactccc agaaggccgc ggggggtgga ccgcctaaga
      181 gggcgtgcgc tcccgacatg ccccgcggcg cgccattaac cgccagattt gaatcgcggg
      241 acccgttggc agaggtggcg gcggcggcat gtactagaga ttaaagagga gaaatactag
      301 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      361 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
      421 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      481 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
      541 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
      601 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
      661 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
      721 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
      781 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
      841 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      901 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
      961 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
     1021 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1081 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1141 gcctttctgc gtttata
//