BBa_K1255003
1
BBa_K1255003
pConst + Riboswitch + BSMT1
2014-06-15T11:00:00Z
2015-05-08T01:09:45Z
BSTM1 opt. comes from <i>Petunia x hybrida</i>
BSMT1 activated with a constitutive promotor, regulated a riboswitch
Description: BSMT1 opt. (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 2mM of salicylic acid to produce a winter green aroma. Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a Riboswitch (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32?? C.
false
false
_1611_
0
19659
9
It's complicated
false
BSTM1 opt. was optimized for its expression in <i>E. coli</i>
false
Antonio Cardenas, Irina Romero, Dulce Valdez
component2379132
1
BBa_J23119
component2379136
1
BBa_K1255001
component2379134
1
BBa_K115017
annotation2379136
1
BBa_K1255001
range2379136
1
133
1206
annotation2379132
1
BBa_J23119
range2379132
1
1
35
annotation2379134
1
BBa_K115017
range2379134
1
44
126
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1255001
1
BBa_K1255001
BSMT1 Opt.
2014-06-12T11:00:00Z
2015-05-08T01:09:45Z
http://parts.igem.org/Part:BBa_J45004
BBa_J45004 optimized.
According to our tests, there must be added between 10mM and 20mM of salicylic acid to produce a wintergreen aroma. For further information check the experience section in this page.
false
false
_1611_
0
19636
9
Not in stock
false
This CDS has being optimized for <i>E. coli</i>, from the piece BBa_J45004 of the MIT group of 2006.
false
Antonio Cardenas, Irina Romero, Dulce Valdez
annotation2379061
1
BSMT1 Opt.
range2379061
1
1
1074
BBa_K115017
1
BBa_K115017
RNA thermometer (ROSE 32??C)
2008-08-19T11:00:00Z
2015-05-08T01:09:28Z
Based on ROSE RNA thermometer sequences from Rfam database.
Released HQ 2013
An RNA thermometer that theoretically switches on translation at 32 degrees Celcius and switched of translation below that temperature. Still to be tested.
false
false
_223_
0
3006
9
In stock
true
a lot, will be added soon.
true
Bastiaan van den Berg
annotation1972920
1
SD
range1972920
1
77
82
BBa_K1255001_sequence
1
atggaagtggttgaagttctgcacatgaacggtggtaatggtgattcgtcgtatgccaataacagcctggtccaacaaaaagtgattctgatgaccaaaccgattacggaacaggcaatgatcgatctgtatagctctctgttcccggaaaccctgtgcattgcggacctgggctgtagcctgggtgccaacacgtttctggtggtttctcaactggtcaaaatcgtggaaaaagaacgtaaaaaacatggcttcaaaagtccggaattttacttccactttaatgatctgccgggcaacgacttcaataccctgtttcagtcactgggtgcgttccaagaagatctgcgtaaacatattggcgaatcttttggtccgtgctttttcagtggcgtgccgggttccttctatacgcgcctgtttccgtcaaaatcgctgcactttgtgtatagttcctactcactgatgtggctgtcgcaggttccgaacggcatcgaaaacaacaagggtaacatctacatggcacgcaccagcccgctgtctgtgatcaaagcttactacaaacaatacgaaatcgatttcagtaactttctgaaataccgttccgaagaactgatgaaaggcggtaaaatggttctgaccctgctgggccgcgaaagcgaagacccgacgtctaaagaatgctgttatatttgggaactgctggcgatggccctgaacaaactggttgaagaaggtctgattaaagaagaaaaagtcgatgcgttcaatatcccgcagtataccccgagtccggccgaagttaaatacatcgtcgaaaaagaaggttcctttaccatcaaccgtctggaaacgtcacgcgtccattggaatgcgtcgaacaatgagaaaaacggcggttataatgtttcacgttgtatgcgcgcagtggctgaaccgctgctggtttcgcatttcgataaagaactgatggacctggtctttcacaaatacgaagaaatcgtgagcgactgtatgtccaaagaaaacacggaatttatcaatgttatcatctccctgaccaaaatcaattaa
BBa_K115017_sequence
1
ccgggcgcccttcgggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtccagtctttgctcagtggaggat
BBa_K1255003_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagccgggcgcccttcgggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtccagtctttgctcagtggaggattactagatggaagtggttgaagttctgcacatgaacggtggtaatggtgattcgtcgtatgccaataacagcctggtccaacaaaaagtgattctgatgaccaaaccgattacggaacaggcaatgatcgatctgtatagctctctgttcccggaaaccctgtgcattgcggacctgggctgtagcctgggtgccaacacgtttctggtggtttctcaactggtcaaaatcgtggaaaaagaacgtaaaaaacatggcttcaaaagtccggaattttacttccactttaatgatctgccgggcaacgacttcaataccctgtttcagtcactgggtgcgttccaagaagatctgcgtaaacatattggcgaatcttttggtccgtgctttttcagtggcgtgccgggttccttctatacgcgcctgtttccgtcaaaatcgctgcactttgtgtatagttcctactcactgatgtggctgtcgcaggttccgaacggcatcgaaaacaacaagggtaacatctacatggcacgcaccagcccgctgtctgtgatcaaagcttactacaaacaatacgaaatcgatttcagtaactttctgaaataccgttccgaagaactgatgaaaggcggtaaaatggttctgaccctgctgggccgcgaaagcgaagacccgacgtctaaagaatgctgttatatttgggaactgctggcgatggccctgaacaaactggttgaagaaggtctgattaaagaagaaaaagtcgatgcgttcaatatcccgcagtataccccgagtccggccgaagttaaatacatcgtcgaaaaagaaggttcctttaccatcaaccgtctggaaacgtcacgcgtccattggaatgcgtcgaacaatgagaaaaacggcggttataatgtttcacgttgtatgcgcgcagtggctgaaccgctgctggtttcgcatttcgataaagaactgatggacctggtctttcacaaatacgaagaaatcgtgagcgactgtatgtccaaagaaaacacggaatttatcaatgttatcatctccctgaccaaaatcaattaa
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z