##gff-version 3
##sequence-region BBa_K125504 1 996
BBa_K125504	.	start_codon	1	3	.	+	0	ID=annotation1971714;Name=start
BBa_K125504	.	stop_codon	994	996	.	+	0	ID=annotation1971716;Name=inserted second stop
BBa_K125504	.	point_mutation	855	855	.	+	0	ID=annotation1971717;Name=E. coli codon optimized, Ile ATT -> ATC, avoided rare Ile ATA
BBa_K125504	.	stop_codon	991	993	.	+	0	ID=annotation1971715;Name=original stop codon
>BBa_K125504
atggaatcgacaaataaaacctggacagaactcatgactcccctcagtcagttttggctggagtccagtagccaggctt
ggaagaattggtttgacctcatggccaagggcggggccggcgccatgatgggctcggctccccagtcctttgagtcttt
gccccaacagttcctccaatcccagcagttttatggagagttacttaagctttcttttgaagcttggcaaagcctgtgg
cctaaattggataatggttcggcgccaggggcagtgcagggctacctaaaacagctacaaacccaaattgagcaatata
ccgccaccacccaagctctccaaggggacatggatggtttatggcagtgttacatcaaggaagtacaaagattttccca
actctggctctccacctggcagagtagcgtcgcccccctgggcaaattacccaccggggacatccatgcttggttagat
ttaaataatctctacggcgatgccctctacaacaaaaacctgagcagttttatgcgatcgcctttgctggggcccagtc
gggaaatgaatggcaaattattgcgggcctttgacgattgggttaagttatcccaggccatggcagactatcaattact
ggaagcagatattcaataccggggctttgctgctttgatggaagatttactggcccgggctaaggaagataaacccgtt
aaaacctggaaggaatttcaacaacggtgggcgatcgccgctgaccaagtgtttgaagaagctttttgtgaggaaaaaa
atctgaaagtacggggcaaattcatcaatgccttgaatcgttatcgcattcagcaacaggagatcctagaagcatggtt
aaaaatgctgaacctccctacccgctcagaggtggatgaaattcatcaaaccatttatcagttgcggaaagaagttaaa
agtttgaaaaaacgattgggagaaacagaagcaaacccaggctaataa