LOCUS       BBa_K1256002            3166 bp    DNA     linear       22-Nov-2024
DEFINITION  pSB1C3-Plac-NB-Cm-MN
ACCESSION   BBa_K1256002
VERSION     BBa_K1256002.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..932
                     /label=BBa_E1010
     stem_loop       947..1075
                     /label=BBa_B0015
     misc_feature    1335..1948
                     /label=ori
     RBS             2256..2915
                     /label=Chloramphenicol resistance cassette
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa gctagcatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 ttaataacgc tgatagtgct agtgtagatc gcggatccta ctagagccag gcatcaaata
      961 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1021 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta
     1081 gtagcggccg ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc cctttttctt
     1141 taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac tcgctgcgct
     1201 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1321 accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct gacgagcatc
     1381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1981 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2041 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2101 ctgacagctc gaggcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
     2161 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagcg
     2221 atgcatagcg gccgccaatt gagctcgata tcaaattacg ccccgccctg ccactcatcg
     2281 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
     2341 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     2401 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     2461 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     2521 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     2581 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     2641 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
     2701 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     2761 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2821 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     2881 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2941 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     3001 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     3061 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     3121 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagag
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