LOCUS       BBa_K1256003            3214 bp    DNA     linear       30-Dec-2025
DEFINITION  pSB1C3-Plac-SS-NB
ACCESSION   BBa_K1256003
VERSION     BBa_K1256003.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..220
                     /label=BBa_B0034
     CDS             296..1001
                     /label=BBa_E1010
     CDS             227..289
                     /label=OmpA Secretion Signal
     stem_loop       1016..1144
                     /label=BBa_B0015
     misc_feature    1404..2017
                     /label=ori
     CDS             2304..2963
                     /label=Chloramphenicol resistance cassette
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaaaacggc
      241 cattgctatc gctgtcgcgt tggcgggctt cgcgactgta gctcaggcgg ctagcatggc
      301 ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt
      361 taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca
      421 gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc
      481 cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca tcccggacta
      541 cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact tcgaagacgg
      601 tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca tctacaaagt
      661 taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa aaaccatggg
      721 ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa
      781 aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa ccacctacat
      841 ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac tggacatcac
      901 ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc
      961 caccggtgct taataacgct gatagtgcta gtgtagatcg cggatcctac tagagccagg
     1021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1081 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     1141 tatatactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc
     1201 ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg ctcactgact
     1261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc cgcccccctg
     1441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2161 aaacttggtc tgacagctcg aggcttggat tctcaccaat aaaaaacgcc cggcggcaac
     2221 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     2281 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     2341 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     2401 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     2461 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     2521 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     2581 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2641 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2701 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2761 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2821 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2881 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     2941 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     3001 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc
     3061 ccgatcaact cgagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     3121 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     3181 ggatgatttc tggaattcgc ggccgcttct agag
//