LOCUS       BBa_K1256004            3135 bp    DNA     linear       23-Nov-2024
DEFINITION  pSB1C3-Plac-SS-Cecropin-MprF
ACCESSION   BBa_K1256004
VERSION     BBa_K1256004.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..289
                     /label=OmpA secretion signal
     CDS             290..397
                     /label=cecropin
     RBS             404..415
                     /label=BBa_B0034
     CDS             422..2992
                     /label=MprF
     stem_loop       3007..3135
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaaaacggc
      241 cattgctatc gctgtcgcgt tggcgggctt cgcgactgta gctcaggcgg gattaaaaaa
      301 actgggtaaa aaattagagc gcattggtaa acatgtcttc aaagcatccg aaaaagcact
      361 gcctgttgtc gccggctata atgcgatcgg caaataagct agcaaagagg agaaatacta
      421 gatgctgatt aaaaagaatg ctttatcaat attaaaaatc gtttttccta ttgctgtttt
      481 gctatttgtt atttatcaat cgaaaaaaga actgacaaat ctgtcattca aacgtacgct
      541 catggtcatc aacggactgg aacgaacgga tttattcatg cttgtgttga tcggcttgct
      601 ggctgttgcg gctatgtcgc tgtatgatta cgtcctgaag tactcactgc gcctatcgat
      661 cacaaacgga aaagttttca gggtttcctg gatcgccaat tcatttaata atgtgttggg
      721 attcggcggt ttagccggag tcgggttaag aatgatgttc tataaggagc atacgaaaga
      781 ccataaggca ctcgtgaaag gaatcgcctg gctcacatca tctatgctgc tcggattatc
      841 tgttttcagc attttcgttg ctgcgagagt gctgccagtt gatgaagtga ttcatgagaa
      901 gccttggctg tgggcggtcg ttatcggttt tgcactgata ttgccgttat ctttagcggt
      961 gtccaaaata aaagaccgca aagctgggga cgaagagaat gcggataaag tgaaaaatcc
     1021 gattttcgca tatattggag cttcagttgt tgaatggctc atggccggga ccgtcatcta
     1081 ttttgctttg ttcgctatgg gcattcatgc agatatcagg tatgtgttcg gggtgttcgt
     1141 cattgcggcg atcggaggaa tgatcagcct cgtgccgggc ggcttcggct cgtttgacct
     1201 tttatttttg ctggggatgg agcagcttgg ctatcatcaa gaggccatcg ttacttctat
     1261 tgtgttgtac aggctcgcct actcatttat cccatttatc ttgggactgt tctttgccgc
     1321 cggcgacctg acggaaaata caatgaagcg gctcgaaacg aacccgcgca tcgcaccggc
     1381 aattgagacg acaaacgttc tgcttgttgt tcagcgtgcg gtattagtga gaattttgca
     1441 aggctcgctt tcccttattg tgttcgtagc aggtctgatt gtcttggcct cagtttcctt
     1501 gccgattgac aggctgacgg ttataccgca cattccgcgc ccggcgcttt tgctgttcaa
     1561 cggcctgtcc ttaagctcag cgctcattct gctcattttg ccgatcgagc tttataaacg
     1621 gacaaaacgt tcctacacga tggccattac agcgcttgtc ggcggctttg tgttcagctt
     1681 tttaaaaggg cttaacatca gcgcgatatt cgtactgccg atgattatcg tattgcttgt
     1741 gctattgaaa aaacaatttg tccgcgaaca ggcatcctat acactgggac aattgatatt
     1801 cgctgtggcg ctgtttactg tggcgctctt taactacaat ctgatcgcgg gctttatttg
     1861 ggaccggatg aagaaggtgc tgcgtcacga atatttcgtc cacagcacct cgcatattac
     1921 acatgcaacc atcatggcga tcatcattgt gccgctgttc ttcttgatat ttacagtggt
     1981 ctatcataag agaacgaaac cgatcggaga gaaagctgac cctgagcgtc ttgctgcgtt
     2041 tctcaatgaa aaaggcggca acgcgctgag ccatcttggt tttcttggag ataagcggtt
     2101 ttatttttct agcgatggaa atgcactgct tctgtttggg aaaatcgcca gaaggctggt
     2161 cgtgctcggc gatccatctg gccaaagaga atcattcccg ctcgtgctgg aagaatttct
     2221 gaacgaagcg catcagaagg gattcagtgt tttgttctat caaattgaac gagaggacat
     2281 ggcgctgtat cacgattttg gctacaactt ctttaaattg ggtgaggaag catatgtaga
     2341 tttaaataca tttaccttga ctgggaagaa aaaagccggc cttcgggcaa tcaataaccg
     2401 ctttgagcgg gaggagtata ctttccatgt ggatcatccc ccattttctg atgcgttttt
     2461 ggaggagctg aagcaaatct cagacgaatg gctcggctcg aaaaaagaga agggattctc
     2521 gctcggattt tttgatcctt cctatttaca gaaagcgccg atcgcctata tgaaaaatgc
     2581 agaaggagag atcgttgcat tcgcaaatgt catgccgatg taccaggaag gagagatatc
     2641 ggtcgatctg atgcgctatc gcggcgacgc tccaaatggc attatggacg cattgtttat
     2701 ccgtatgttt ttatgggcaa aggaagaggg ctgtacgtca tttaatatgg ggatggcacc
     2761 cttggccaat gtcggcactg cctttacatc cttctggtcc gaaaggtttg ccgctgtcat
     2821 ttttaataat gtcagataca tgtacagttt cagcggccta agagccttta aagaaaaata
     2881 taaaccggag tggcgaggga aatacttagc gtatcggaaa aacagatctc tttctgtcac
     2941 catgttcctc gttacacgtc tgattggcaa aagcaaaaaa gactccgtct aaggatccta
     3001 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     3061 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     3121 ctttctgcgt ttata
//